冀豆12遗传背景下3个回交组合高低蛋白含量 后代品系SSR标记分析

doi:10.3864/j.issn.0578-1752.2014.02.003

Abstract

Abstract: 【Objective】 The objective of this study is to investigate the QTL and their chromosomal segments that determine the seed protein content of progeny strains of high-protein and low protein content, which derived from the crosses between high combing-ability cultiver Jidou 12 and three other cultrivers. By accomplishment of these analyses, the results will provide a basis for the use of high-protein breeding. 【Method】 High protein germplasm materials with protein content more than 50% were created by backcrossing strategy using Jidou 12 as recurrent parent varieties and three different varieties as donor parents. Four high-protein (50%-53%) strains and three low protein (38%-41%) strains were selected from propeny strains of three backcrosses (Hongfeng 11, Chamoshidou and Suinong 14 as the donor parent of three backcrosses). These strains had the same agronomic traits and had no significant difference with Jidou 12. Differences in QTL loci between progeny strains and Jidou12 were analyzed by using 209, 201, and 199 polymorphic SSR markers, respectively, in strains from three backcrosses. Degrees in QTL expression and sharing in terms of protein contents among different strains and crosses were investigated. 【Result】 The high-protein strains had better genetic recovery than that of low protein strains in the same backcross progeny. The similarity coefficient between two high protein strains, H116 and H117 from Jidou12 × Chamoshidou, and Jidou12, were 83.08% and 84.04%, respectively, which was 71.14% higher than that of the low protein strains. The average of similarity coefficient between four high protain strains and Jidou12 was 79.58%, significantly higher than that of three low protein strains and Jidou12. Linkage analyses using SSR markers indicated that high-protein lines and low-protein lines had remarkable differences in genetic locations. The major difference was inherited from Jidou 12 , which is 27 % (23 loci) on average. SSR markers were initially linked to 22 genomic regions in the three crosses, including 18 for increasing the protein content with allelic variation from Jidou 12. Further analysis identified four different loci in the three backcross population for high-and-low protein chromosome segments: D2 linkage group between 67.71 - 84.18 cM, G chain group between 80.38 - 96.57 cM, C1 linkage group between 75.52 - 80.62 cM, and I chain group bewteen 46.22 - 50.11 cM. 【Conculusion】 Jidou 12 contains a large number of high-protein QTL genetic loci and provides an essential genetic background in high-protein breeding. High-protein genetic loci from donor parents were detected by SSR markers in the progeny populations with Jidou12 as the recurrent parent. This study lays a foundation for excavating excellent genes and high-protein breeding.

Key words: Jidou12 , protein content , SSR markers , chromosome segment

CHEN Qiang-1, 2 , YAN Long-1, YANG Chun-Yan-1, ZHANG Jia-Nan-1, SHI Xiao-Lei-1, DONG Fang-Yang-2, DENG Ying-Ying-1, 2 , HOU Wen-Huan-1, ZHANG Meng-Chen-1, 2 . SSR Markers Linked to High and Low Protein Content Strains Derived from 3 Backcross Combinations Under Jidou 12 Genetic Background[J].Scientia Agricultura Sinica, 2014, 47(2): 230-239.

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SSR Markers Linked to High and Low Protein Content Strains Derived from 3 Backcross Combinations Under Jidou 12 Genetic Background

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