Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (18): 3834-3841.doi: 10.3864/j.issn.0578-1752.2013.18.012

• HORTICULTURE • Previous Articles     Next Articles

Lenticel Blotch Pit and Ca Nutrition, Distribution and Localization in ‘Fuji’ Apple Fruit

 DONG  Yu-12, FENG  Yun-Xiao-1, GUAN  Jun-Feng-1   

  1. 1.Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051
    2.College of Life Sciences, Hebei Normal University, Shijiazhuang 050016
  • Received:2013-06-24 Online:2013-09-15 Published:2013-07-08

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Abstract: 【Objective】 In order to investigate the content and micro-zone distribution of Ca, K and Mg in different parts of apple fruits with lenticel blotch pit and healthy fruits, and to analyze the relationship between Ca localization and lenticel blotch pit occurrence, thus, to clarify the cause of lenticel blotch pit in apple. 【Method】Apple (Malus domestica cv. Spur Fuji) with lenticel blotch pit and healthy fruits were used to determine the content and micro-zone distribution of Ca, K and Mg in fruit using atomic absorption spectrometry and X-ray energy spectra, respectively, and the cellular Ca localization was observed using ultracytochemical method.【Result】Ca content in peel tissue of apple fruit with lenticel blotch pit was significantly lower than that in healthy fruit. However, there were no significant difference in K and Mg contents between lenticel blotch pit fruit and healthy one. The results of X-ray microanalysis showed that the energy spectra intensity peak of Ca and K in peel tissue of healthy fruit were significantly higher than that of the one with lenticel blotch pit, but in the pulp tissue, the peaks of Ca and K were higher and lower in healthy fruit than that with lenticel blotch pit, respectively. And, the highest of K peak appeared in the zone of lenticel blotch pit. Numerous of Ca grains with uniform distribution were deposited in the vacuole of peel and pulp tissue in the healthy fruit. However, less Ca grains were observed in fruits with lenticel blotch pit, and especially, lots of Ca grains were showed at outside of tonoplast in the zone of lenticel blotch pit in peel tissue, and more, the cells shapes were irregular with lower inclusions, and formed some different sizes of vesicles.【Conclusion】Ca content in peel tissue of fruits with lenticel blotch pit was significantly lower than that in the healthy fruits. A lot of K accumulated in zone of lenticel blotch pit in fruit flesh. Intracellular Ca was reduced and got out of the cell in fruits with lenticel blotch pit, which revealed that Ca involved in and influenced the occurrence of the lenticel blotch pit.

Key words: ‘Fuji&rsquo, apple , lenticel blotch pit , Ca nutrition , element distribution

[1]陈策. 苹果苦痘病和其他几种果实钙营养失调症. 落叶果树，2006(3): 10-13.

Chen C. Bitter pit in apple fruit and several other calcium dystrophies. Deciduous Fruits, 2006(3):10-13. (in Chinese)

[2]Fuller M M. Cell ultrastructure in apple fruits in relation to calcium concentration and fruit quality. Acta Horiculturae, 1980, 92: 51-55.

[3]De Freitas S T, Do Amarante C V T, Labavitch J M, Mitcham E J. Cellular approach to understand bitter pit development in apple fruit. Postharvest Biology and Technology, 2010, 57(1): 6-13.

[4]Ferguson J B, Watkins C B. Bitter pit in apple fruit. Horticultural Reviews, 1989, 11: 289-356.

[5]Bangerth F. Calcium-related physiological disorders of plants. Annual Review of Phytopathology, 1979, 17: 97-122.

[6]Dris R, Niskkanen R, Falahi E. Nitrogen and calcium nutrition and fruit quality of commercial apple cultivars grown in Finland. Journal of Plant Nutrition, 1998, 21(11): 2389-2402.

[7]Helper P K, Wayne R O. Calcium and plant development. Annual Review of Phytopathology, 1985, 36: 397-439.

[8]周卫，李书田，林葆，张新生，林继雄，谢志霄，赵彦卿，艾卫，乔国梅. 喷钙对苹果果实生理特性的影响. 土壤肥料，2000(6): 25-28.

Zhou W, Li S T, Lin B, Zhang X S, Lin J X, Xie Z X, Zhao Y Q, Ai W, Qiao G M. Effect of calcium spraying on physiological characteristics of apples. Soils and Fertilizers, 2000(6): 25-28. (in Chinese)

[9]王春枝，安宁，许大志，刘丽洁，徐冰，尹东波，邰日晶. 钙镁肥配施对苹果树叶片和果实营养元素含量及病果率影响. 北方园艺，2010(14): 23-26.

Wang C Z, An N, Xu D Z, Liu L J, Xu B, Yin D B, Tai R J. The effect of different calcium and magnesium fertilizer treatment on apple nutrient content and rate of disease. Northern Horticulture, 2010(14): 23-26. (in Chinese)

[10]Peryea F J, Nelsen G H, Faubion D. Start-timing for calcium chloride spray programs influences fruit calcium and bitter pit in ‘Braeburn’ and ‘Honeycrisp’ apples. Journal of Plant Nutrition, 2007, 30(8): 1213-1227.

[11]Blanco A, Fernández V, Val J. Improving the performance of calcium-containing spray formulations to limit the incidence of bitter pit in apple (Malus × domestica Borkh.). Scientia Horticulturae, 2010, 127(1): 23-28.

[12]Burmeister D M, Dilley D R. Induction of bitter pit-like symptoms on apples by infiltration with Mg2+ is attenuated by Ca2+. Postharvest Biology and Technology, 1991, 1(1): 11-17.

[13]Shorrocks V M, Nicholson D D. The influence of boron deficiency on fruit quality. Acta Horticultuae, 1980, 92: 103-110.

[14]Casero T, Benavides A L, Recasens I. Interrelation between fruit mineral content and pre-harvest calcium treatment on ‘Golden Smoothee’ apple quality. Journal of Plant Nutrition, 2010, 33(1): 27-37.

[15]仝月澳，周厚基. 果树营养诊断法. 北京：农业出版社，1982.

Tong Y A, Zhou H J. Nutrient Diagnosis of Fruit Tree. Beijing: Agricultural Press, 1982. (in Chinese)

[16]Kerton M, Newbury H J, Hand D, Pritchard J. Accumulation of calcium in the centre of leaves of coriander (Coriandrum sativum L.) is due to an uncoupling of water and ion transport. Journal of Experimental Botany, 2009, 60(1): 227-235.

[17]Suzuki K, Shono M, Egawa Y. Localization of calcium in the pericarp cells of tomato fruits during the development of blossom-end rot. Protoplasm, 2003, 222(3/4): 149-156.

[18]Picchioni G A, Watada A E, Whitaker B D, Reyes A. Calcium delays senescence-related membrane lipid changes and increases net synthesis of membrane lipid components in shredded carrots. Postharvest Biology and Technology, 1996, 9(2): 235-245.

[19]Picchioni G A, Watada A E, Conway W S, Whitaker B D, Sams C E. Postharvest calcium infiltration delays membrane lipid catabolism in apple fruit. Journal of Agricultural and Food Chemistry, 1998, 46(7): 2452-2457.

[20]Sió J, Boixadera J , Rosera J. Effect of orchard factors and mineral nutrition on bitter pit in ‘Golden Delicious’ apples. Acta Horticulturae, 1999, 485: 331-334.

[21]范业宏，高树青，刘秀春，王炳华，陈宝江. 不同钙肥防治苹果苦痘病的效果. 北方果树，2011(1): 9-10.

Fan Y H, Gao S Q, Liu X C, Wang B H, Chen B J. Effects of different calcium fertilizers on the control of apple bitter pit. Northern Fruits, 2011(1): 9-10. (in Chinese)

[22]Retamales J B, Lepe V P. Control strategies for different bitter pit incidences in braeburn apples. Acta Horticulturae, 2000, 517: 227-234.

[23]Fukumoto M, Nagai K. Possible roles of calcium and ammonium in the development of bitter pit in apple. Physiologia Plantarum, 1983, 59(2): 171-176.

[24]Simons R K, Chu M C. Scanning electron microscopy and electron microprobe studies of bitter pit in apples. Acta Horticulturae, 1980, 92: 57-70.

[25]Martinoia E, Maeshima M, Neuhaus H E. Vacuolar transporters and their essential role in plant metabolism. Journal of Experimental Botany, 2007, 58(1): 83-102.
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