‘富士’苹果痘斑病与Ca营养、分布和定位

doi:10.3864/j.issn.0578-1752.2013.18.012

Abstract

Abstract: 【Objective】 In order to investigate the content and micro-zone distribution of Ca, K and Mg in different parts of apple fruits with lenticel blotch pit and healthy fruits, and to analyze the relationship between Ca localization and lenticel blotch pit occurrence, thus, to clarify the cause of lenticel blotch pit in apple. 【Method】Apple (Malus domestica cv. Spur Fuji) with lenticel blotch pit and healthy fruits were used to determine the content and micro-zone distribution of Ca, K and Mg in fruit using atomic absorption spectrometry and X-ray energy spectra, respectively, and the cellular Ca localization was observed using ultracytochemical method.【Result】Ca content in peel tissue of apple fruit with lenticel blotch pit was significantly lower than that in healthy fruit. However, there were no significant difference in K and Mg contents between lenticel blotch pit fruit and healthy one. The results of X-ray microanalysis showed that the energy spectra intensity peak of Ca and K in peel tissue of healthy fruit were significantly higher than that of the one with lenticel blotch pit, but in the pulp tissue, the peaks of Ca and K were higher and lower in healthy fruit than that with lenticel blotch pit, respectively. And, the highest of K peak appeared in the zone of lenticel blotch pit. Numerous of Ca grains with uniform distribution were deposited in the vacuole of peel and pulp tissue in the healthy fruit. However, less Ca grains were observed in fruits with lenticel blotch pit, and especially, lots of Ca grains were showed at outside of tonoplast in the zone of lenticel blotch pit in peel tissue, and more, the cells shapes were irregular with lower inclusions, and formed some different sizes of vesicles.【Conclusion】Ca content in peel tissue of fruits with lenticel blotch pit was significantly lower than that in the healthy fruits. A lot of K accumulated in zone of lenticel blotch pit in fruit flesh. Intracellular Ca was reduced and got out of the cell in fruits with lenticel blotch pit, which revealed that Ca involved in and influenced the occurrence of the lenticel blotch pit.

Key words: ‘Fuji&rsquo, apple , lenticel blotch pit , Ca nutrition , element distribution

DONG Yu-12, FENG Yun-Xiao-1, GUAN Jun-Feng-1. Lenticel Blotch Pit and Ca Nutrition, Distribution and Localization in ‘Fuji’ Apple Fruit[J].Scientia Agricultura Sinica, 2013, 46(18): 3834-3841.

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Lenticel Blotch Pit and Ca Nutrition, Distribution and Localization in ‘Fuji’ Apple Fruit

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