Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (16): 3478-3487.doi: 10.3864/j.issn.0578-1752.2013.16.019

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning, Evolution and Expression Features of MAPK1 Gene Family from Brassica Species (B. napus, B. oleracea, B. rapa)

 LU  Jun-Xing, LU  Kun, ZHU  Bin, PENG  Qian, LU  Qi-Feng, QU  Cun-Min, YIN  Jia-Ming, LI  Jia-Na, LIANG  Ying, CHAI  You-Rong   

  1. College of Agronomy and Biotechnology, Southwest University/Chongqing Rapeseed Engineering & Technology Research Center/Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715
  • Received:2013-03-11 Online:2013-08-15 Published:2013-05-29

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Abstract: 【Objective】The objective of this study is to isolate MAPK1 gene family from Brassica napus, Brassica oleracea and Brassica rapa and analyze the transcriptional expression profiles of MAPK1 in various organs of these three species.【Method】On the basis of in silico cloning, the full-length cDNA and gDNA of MAPK1 genes were isolated from the three species using RACE (Rapid Amplification of cDNA Ends) technology. Organ specificity of MAPK1 in various organs of the three species was detected by quantitative real-time PCR (qRT-PCR).【Result】In this study, four MAPK1 genes including BnMAPK1-1, BnMAPK1-2, BoMAPK1 and BrMAPK1 were isolated from B. napus, B. oleracea and B. rapa. Their gDNA sequences were 2 512-2 525 bp, containing three exons and two introns. Standard mRNA lengths of these genes were 1 599-1 620 bp, all with an ORF of 1 100 bp. Phylogenetic tree showed that BnMAPK1 gene family was originated from the assemblage of B. oleracea and B. rapa MAPK1 genes, BnMAPK1-1 and BnMAPK1-2 corresponding to BoMAPK1 and BrMAPK1, respectively. qRT-PCR showed that MAPK1 genes expressed in all tested organs, but organ specificity showed significant differences among species implying quick evolution.【Conclusion】 In this study, the full-length cDNA and gDNA of MAPK1 genes were isolated from B. napus, B. oleracea and B. rapa. It supports the assumption that B. napus is a heterotetraploid species of B. oleracea and B. rapa by natural interspecific hybridization. Brassica MAPK1 genes show much conservation in gene and protein sequences and structures, but the transcription organ specificity evolves very fast and show significant differences among closely related species.

Key words: B. napus , B. oleracea , B. rapa , cloning , evolution , expression , MAPK1

[1]Bogre L, Meskiene I, Heberle-Bors E, Hirt H. Stressing the role of MAP kinases in mitogenic stimulation. Plant Molecular Biologyl, 2000, 43: 705-718.

[2]Tena G, Asai T, Chiu W L, Sheen J. Plant mitogen-activated protein kinase signaling cascades. Current Opinion in Plant Biology 2001, 4: 392-400.

[3]Zhang S, Klessig D F. MAPK cascades in plant defense signaling. Trends in Plant Science, 2001, 6: 520-527.

[4]Morris P C. MAP kinase signal transduction pathways in plants. New Phytologist, 2001, 151: 67-89.

[5]Ortiz-Masia D, Perez-Amador M A, Carbonell J, Marcote M J. Diverse stress signals activate the C1 subgroup MAP kinases of Arabidopsis. Febs Letters, 2007, 581: 1834-1840.

[6]Morinaga T. Interspecific hybridization in Brassica: I. The cytology of F1 hybrids of B. napella and various other species with 10 chromosomes. Cytologia, 1929, 1: 16-27.

[7]Morinaga T. Interspecific hybridization in Brassica: VI. The cytology of F1 hybrids of B. juncea and B. nigra. Cytologia, 1934, 6: 62-67.

[8]Nagaharu U. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japanese Journal of Botany, 1935, 7: 389-452.

[9]Organisation for Economic Co-operation and Development. Series on harmonization of regulatory oversight in biotechnology No.7[R]. Consensus document on the biology of Brassica napus L. (oilseed rape), 52178, Paris: OCED, 1997.

[10]Ichimura K, Shinozaki K, Tena G, Sheen J, Henry Y, Champion A, Kreis M, Zhang S Q, Hirt H, Wilson C, Heberle-Bors E, Ellis B E, Morris P C, Innes R W, Ecker J R, Scheel D, Klessig D F, Machida Y, Mundy J, Ohashi Y, Walker J C. Mitogen-activated protein kinase cascades in plants: A new nomenclature. Trends in Plant Science, 2002, 7: 301-308.

[11]Ortiz-Masia D, Perez-Amador M A, Carbonell P, Aniento F, Carbonell J, Marcote M J. Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.). Planta, 2008, 227: 1333-1342.

[12]Mizoguchi T, Gotoh Y, Nishida E, Yamaguchi-Shinozaki K, Hayashida N, Iwasaki T, Kamada H, Shinozaki K. Characterization of 2 cDNAs that encode MAP Kinase Homologs in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells. The Plant Journal, 1994, 5: 111-122.

[13]Mizoguchi T, Irie K, Hirayama T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K, Shinozaki K. A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93: 765-769.

[14]Umezawa T, Sugiyama N, Takahashi F, Anderson J C, Ishihama Y, Peck S C, Shinozaki K. Genetics and phosphoproteomics reveal a protein phosphorylation network in the abscisic acid signaling pathway in arabidopsis thaliana. Science Signaling, 2013, 6: 1-13.

[15]Chen X, Truksa M, Shah S, Weselake R J. A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus. Analytical Biochemistry, 2010, 405: 138-140.

[16]Bustin S A, Benes V, Garson J A, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl M W, Shipley G L, Vandesompele J, Wittwer C T. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 2009, 55: 611-622.

[17]Yang Y W, Lai K N, Tai P Y, Ma D P, Li W H. Molecular phylogenetic studies of Brassica, rorippa, arabidopsis and allied genera based on the internal transcribed spacer region of 18S-25S rDNA. Molecular Phylogenetics and Evolution, 1999, 13: 455-462.

[18]Schmidt R, Acarkan A, Boivin K. Comparative structural genomics in the Brassicaceae family. Plant Physiology and Biochemistry, 2001, 39: 253-262.

[19]Lysak M A, Koch M A, Pecinka A, Schubert I. Chromosome triplication found across the tribe Brassiceae. Genome Research, 2005, 15: 516-525.

[20]Mun J H, Kwon S J, Yang T J, Seol Y J, Jin M, Kim J A, Lim M H, Kim J S, Baek S, Choi B S, Yu H J, Kim D S, Kim N, Lim K B, Lee S I, Hahn J H, Lim Y P, Bancroft I, Park B S. Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplication. Genome Biology, 2009, 10: R111.

[21]Xu B B, Li J N, Zhang X K, Wang R, Xie L L, Chai Y R. Cloning and molecular characterization of a functional flavonoid 3'-hydroxylase gene from Brassica napus. Journal of Plant Physiology, 2007, 164: 350-363.

[22]Chen A H, Chai Y R, Li J N, Chen L. Molecular cloning of two genes encoding cinnamate 4-hydroxylase (C4H) from oilseed rape (Brassica napus). Journal of Biochemistry and Molecular Biology, 2007, 40: 247-260.

[23]Ni Y, Jiang H L, Lei B, Li J N, Chai Y R. Molecular cloning, characterization and expression of two rapeseed (Brassica napus L.) cDNAs orthologous to Arabidopsis thaliana phenylalanine ammonia-lyase 1. Euphytica, 2008, 159: 1-16.

[24]Lu K, Chai Y R, Zhang K, Wang R, Chen L, Lei B, Lu J, Xu X F, Li J N. Cloning and characterization of phosphorus starvation inducible Brassica napus PURPLE ACID PHOSPHATASE 12 gene family, and imprinting of a recently evolved MITE-minisatellite twin structure. Theoretical and Applied Genetics, 2008, 117: 963-975.

[25]Chai Y R, Lei B, Huang H L, Li J N, Yin J M, Tang Z L, Wang R, Chen L. TRANSPARENT TESTA 12 genes from Brassica napus and parental species: cloning, evolution, and differential involvement in yellow seed trait. Molecular Genetics and Genomics, 2009, 281: 109-123.

[26]卢坤, 张凯, 柴友荣, 陆俊杏, 唐章林, 李加纳. 甘蓝和白菜紫色酸性磷酸酶17基因家族的克隆和比较分析. 作物学报, 2010, 36(3): 517-525.

Lu K, Zhang K, Chai Y R, Lu J X, Tang Z L, Li J N. Cloning and comparative analysis of PURPLE ACID PHOSPHATASE 17 gene families in Brassica oleracea and Brassica rapa. Acta Agronomica Sinica, 2010, 36(3): 517-525. (in Chinese)
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