Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2769-2776.doi: 10.3864/j.issn.0578-1752.2013.13.014

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effect of Quercetin on cAMP Signaling Pathway in Chicken Adipocytes

 欧Yang-Wen-Wen , LI  Yao, ZHAO  Wei, WANG  Ming-Hao, JIN  Fang   

  1. Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030
  • Received:2012-12-24 Online:2013-07-01 Published:2013-05-08

PDF

723

Cited

3

Abstract: 【Objective】This experiment was conducted to study the effects of quercetin on cAMP signaling pathway in broilers adipocytes. 【Method】 Adipocytes were randomly allocated into 5 groups with 6 replicates per group. Adipocytes were cultured in basal medium, 2‰ DMSO medium or addition of 10, 20, 40 mg•L-1 quercetin medium for 24 h, 48 h and 72 h, respectively. Then adipocytes and medium were collected for measuring the contents of ACC, FAS, LPL, cAMP, AC, PDE, PKA by ELISA and TG by ultraviolet spectrophotometer, respectively.【Result】Compared with the control, all of these indicators of adipocytes were not significantly affected by 2‰ DMSO (P＞0.05). The contents of ACC, FAS, LPL,TG, and PDE were significantly decreased in medium supplemented with 20 mg•L-1 and 40 mg•L-1 quercetin (P＜0.05), cAMP and PKA contents were significantly increased (P＜0.05); Intracellular AC content was not significantly affected by quercetin (P＞0.05).【Conclusion】The results indicated that quercetin inhibited lipogenesis and fat deposition via regulating cAMP signaling pathway.

Key words: quercetin , adipocytes , lipometabolism , cAMP , signaling pathway

[1]李莉, 朱晓彤, 束刚, 高萍, 江青艳, 陈阳城. 日粮中添加山楂叶总黄酮对黄羽肉鸡脂肪代谢的影响. 江西农业大学学报, 2009, 31(4): 600-615.

Li L, Zhu X T, Shu G, Gao P, Jiang Q Y, Chen Y C. Effett of hawthorn leaf flavonoids on lipid metabolism in yellow-feathered broilers. Acta Agriculturae Universitatis Jiangxiensis, 2009, 31(4): 600-615. (in Chinese)

[2]周萍芳. 苜蓿黄酮提取物对麻鸡生产性能及脂肪沉积的影响[D]. 南昌: 江西农业大学, 2011.

Zhou P F. Effects of Crude extracts of alfalfa flabone on growth performance and lipid de0osit of broilers[D]. Nanchang: Jiangxi Agricultural University, 2011. (in Chinese)

[3]李垚, 付晶, 王宝东, 王艳波, 单安山. 沙棘黄酮对AA肉仔鸡胴体和肉品质的影响. 畜牧兽医学报, 2008, 39(9): 1217-1223.

Li Y, Fu J, Wang B D, Wang Y B, Shan A S. Effect of flavones of sea buckthorn on carcass characteristics and meat quality of arbor acres broilers. Acta Veterinaria et Zootechnica Sinica, 2008, 39(9): 1217-1223. (in Chinese)

[4]夏蕾, 张志宏, 左金国, 刘长伟, 金赛勉, 李垚. 沙棘提取物对猪脂肪中部分脂肪代谢相关基因表达的影响. 营养学报, 2009, 31(2): 177-180.

Xia L, Zhang Z H, Zuo J G, Liu C W, Jin S M, Li Y. Effects of sea buckthorn extract on expression of porcine genes related to lipid metabolism. Acta Nutrimenta Sinica, 2009, 31(2): 177-180. (in Chinese)

[5]Odbayar T, Kimura T, Tsushida T, Ide T. Isoenzyme-specific up-regulation of glutathione transferase and aldo-keto reductase mRNA expression by dietary quercetin in rat liver. Molecular and Cellular Biochemistry, 2009, 325(1/2): 121-130.

[6]杨波, 任晓丽, 李翔. 槲皮素对力竭运动疲劳大鼠血清自由基代谢的影响. 解放军预防医学杂志, 2011, 29(1): 19-21.

Yang B, Ren X L, Li X. Effects of quercetin on blood serum free radical metabolism in exhausted fatigue. Journal of Preventive Medicine of Chinese People’s Liberation Army, 2011, 29(1): 19-21. (in Chinese)

[7]Kuhlmann C R, Schaefer C A, Kosok C, Abdallah Y, Walther S, Lüdders D W, Neumann T, Tillmanns H, Schäfer C, Piper H M, Erdogan A. Quercetin-induced induction of the NO/cGMP pathway depends on Ca2+-activated K+ channel-induced hyperpolarization- mediated Ca2+ -entry into cultured human endothelial cells. Planta Medica, 2005, 71(6): 520-524.

[8]Kamada C, Dasilve E L, Ohnishi-Kameyama M, Moon J H, Terao J. Attenuation of lipid peroxidation and hyperlipidemia by quercetin glucoside in the aorta of high cholesterol-fed rabbit. Free Radical Research, 2005, 39(2): 185-194.

[9]王秋红, 匡海学, 吴伦, 李阳, 王知斌. 二氢槲皮素对异丙肾上腺素致大鼠心肌缺血的保护作用. 中国实验方剂学杂志, 2011, 17(17): 177-180.

Wang Q H, Kuang H X, Wu L, Li Y, Wang Z B. Protective effect of dihyfroquercetin on acute myocardial ischemia induced by isoproterenol in rats. Chinese Journal of Experimental Traditional Medical Formulae, 2011, 17(17): 177-180. (in Chinese)

[10]Boots A W, Wilms L C, Swennen E L, Kleinjans J C, Bast A, Haenen G R. In vitro and ex vivo anti-inflammatory activity of quercetin in healthy volunteers. Nutrition, 2008, 24(7/8): 703-710.

[11]González-Segovia R, Quintanar J L, Salinas E, Ceballos-Salazar R, Abiles-Jiménez F, Torres-López J. Effect of the flabonoid quercetin on inflammation and lipid peroxidation induced by Helicobacter pylori in gastric mucosa of guinea pig. Gastroenterology, 2008, 43(6): 441-447.

[12]Dudeja V, Mujumdar N, Phillips P, Chuqh R, Borja-Cacho D, Dawra R K, Vickers S M, Saluja A K. Heat shock protein 70 inhibits apoptosis in cancer cells through simultaneous and independent mechanisms. Gastroenterology, 2009, 136(5): 1772-1782.

[13]Lin C, Yu Y, Zhao H G, Yan H, Cui Y. Combination of quercetin with radiotherapy enhances tumor radiosensitivity in vitro and in vivo. Radiotherapy and Oncology, 2011, doi.org/10.1016/j. radonc. 2011. 10.023.

[14]张颖, 张立木, 高永峰, 李同德, 张景新. 泰山沙棘果成分含量测定及降血糖与免疫增强作用. 中国医院药学杂志, 2009, 29(19): 1604-1607.

Zhang Y, Zhang L M, Gao Y F, Li T D, Zhang J X. Content determination and hypoglycemic and imm unity effect of Hippophae rhamnoides taishanensis Rousi. Chinese Journal of Hospital Pharmacy, 2009, 29(19): 1604-1607. (in Chinese)

[15]Carmen G Y, Victor S M. Signalling mechanisms regulating lipolysis. Cell Signal, 2006, 18(4): 401-408.

[16]王丽. 二丁酰环腺苷酸对肥育猪胴体品质和肉质的影响及机理研究[D]. 哈尔滨: 东北农业大学, 2009.

Wang L. Studies on effects and mechanisms of dbcamp on carcass traits and pork quality in finishing pigs[D]. Harbin: Northeast Agricultural University, 2009. (in Chinese)

[17]Lynch M J, Hill E V, Houslay M D. Intracellular targeting of phosphodiesterase-4 underpins compartmenta-lized cAMP signaling. Current Topics in Development Biology, 2006, 75: 225-259.

[18]Cismowski M J, Lanier S M. Activation of heterotrimeric G-proteins independent of a G-protein coupled receptor and the implications for signal processing. Reviews of Physiology, Biochemistry and Pharmacology, 2005, 155: 57-80.

[19]Boswell-Smith V, Spina D, Page C P. Phosphodiesterase inhibitors. British Journal of Pharmacology, 2006, 147(S1): S252-S257.

[20]Ferrel J E, Chang Sing P D, Loew G, King R, Mandour J M, Mansour T E. Structure/activity studies of flavonoids as inhibitors of cyclic AMP phosphodiesterase and relationship to quantum chemical indices. Molecular Pharmacology, 1979, 16(2): 556-568.

[21]林成招, 马海田, 邹思湘, 王国杰, 陈伟华, 韩正庚. 大豆异黄酮对大鼠乳腺癌细胞内cAMP/PKA信号通路途径的影响. 生理学报, 2005, 57(4): 517-522.

Lin C Z, Ma H T, Zou S X, Wang G J, Chen W H, Han Z G. Effect of soy isoflavones on cAMP/PKA pathway in breast cancer cells of the rat. Acta Physiologica Sinica, 2005, 57(4): 517-522. (in Chinese)

[22]Li P G, Sun L, Han X, Gan W T, Xu J W. Quercetin induces rapid eNOS phosphorylation and vasodilation by an Akt-dependent and PKA-dependent mechanism. Pharmacology, 2012, 89(3/4): 220-228.

[23]Chang S Y, Cho J M, Kim D B, Jang H J, Ko S H, Kim M J. Molecular mechanisms of early growth response protein-1(EGR-1) expression by quercetin in INS-1 beta-cells. Journal of Cellular Biochemistry, 2012, 113(5): 1559-1568.

[24]Dell'Agli M, Maschi O, Galli G V, Fagnani R, Dal Cero E, Caruso D, Bosisio E. Inhibition of platelet aggregation by olive oil phenols via cAMP-phosphodiesterase. The British Journal of Nutrition, 2008, 99(5): 945-951.

[25]Tong L. Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cellular and Molecular Life Sciences, 2005, 62(16): 1784-1803.

[26]Karami K J, Coppola J, Krishnamurthy K, Llanos D J, Mukherjee A, Venkatachalam K V. Effect of food deprivation and hormones of glucose homeostasis on the acetyl CoA carboxylase activity in mouse brain: a potential role of acc in the regulation of energy balance. Nutrition and Metabolism, 2006, 3: 15-23.

[27]Wolfgang M J, Lane M D. The role of hypothalamic malonyl-CoA in energy homeostasis. The Journal of Biological Chemistry, 2006, 281(49): 37265-37269.

[28]王丽芳. 添加菊科植物黄花蒿提取物对奶山羊乳中CLA含量影响及机理研究[D]. 呼和浩特: 内蒙古农业大学, 2010.

Wang L F. The study on effects of compositae Artemisia annua extracts on CLA content in goat milk and the relative mechanism[D]. Hohhot: Inner Mongolia Agricultural University, 2010. (in Chinese)

[29]Ha do T, Trung T N, Phuong T T, Yim N, Chen Q C, Bae K. The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells. Bioorganic and Medicinal Chemistry Letters, 2010, 20(20): 6076-6081.

[30]Gnoni G V, Paglialonga G, Siculella L. Quercetin inhibits fatty acid and triacylglycerol synthesis in rat-liver cells. European Journal of Clinical Investigation, 2009, 39(9): 761-768.

[31]Loftus T M, Jaworsky D E, Frehywot G L, Townsend C A, Ronnett G V, Lane M D, Kuhajda F P. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science, 2000, 288(5475): 2379-2381.

[32]刘晓鑫, 田维熙, 马晓丰. 草莓提取物对脂肪酸合酶及脂肪细胞的抑制作用. 中国科学院研究生院学报, 2010, 27(6): 768-777.

Liu X X, Tian W X, Ma X F. Inhibitory effects of strawberry extracts on animal fatty acid synthase. Journal of the Graduate School of the Chinese Academy of Sciences, 2010, 27(6): 768-777. (in Chinese)

[33]张萌, 池田克己, 家森幸男. 染料木黄酮对3T3-L1前脂肪细胞分化的作用及其机制研究. 营养学报, 2009, 31(2): 152-155.

Zhang M, Katsumi I, Yukio Y. Effect of genistein on adipogenesis of 3T3-L1 preadipocytes and its mechanism. Acta Nutrimenta Sinica, 2009, 31(2): 152-155. (in Chinese)

[34]Ahn J, Lee H J, Kim S, Ha T. The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochemical and Biophysical Research Communications, 2008, 373(4): 545-549.

[35]Shan T Z, Wang Y Z, Li M. Cloning of lipoprotein lipase (LPL) gene of swine and the difference of LPL gene expression at different avoirdupois stages. Journal of Agricultural Biotechnology, 2006, 14(2): 151-155.

[36]梁伟伟, 严瑾, 吴利花, 范春雷. 山楂叶总黄酮对高脂血症大鼠脂代谢的影响. 健康研究, 2010, 30(1): 8-11.

Liang W W, Yan J, Wu L H, Fan C L. Effect of hawthorn leaf flavonids on lipid metabolism in hyperlipidemia rats. Health Research, 2010, 30(1): 8-11. (in Chinese)

[37]曹兰秀, 周永学, 顿宝生, 赵娴. 女贞子总黄酮对高脂模型小鼠脂代谢的分子靶点的研究. 中成药, 2010, 32(10): 1789-1790.

Cao L X, Zhou Y X, Dun B S, Zhao X. Privet total flavone on lipid metabolism in hyperlipidemia model mice molecular target research. Chinese Traditional Patent Mdicine, 2010, 32(10): 1789-1790. (in Chinese)

[38]Lee J, Jung E, Lee J, Kim S, Huh S, Kim Y, Kim Y, Byun S Y, Park D. Isorhamnetin represses adipogenesis in 3T3-L1 cells. Obesity (Silber Spring), 2009, 17(2): 226-232.

[39]Lu M, Shyy J Y. Sterol regulatory element-binding protein 1 is negatively modulated by PKA phosphorylation. American Journal of Physiology Cell Physiology, 2006, 290(6): 1477-1486.

[40]Zmuda-Trzebiatowska E, Manganiello V, Degerman E. Novel mechanisms of the regulation of protein kinase B in adipocytes; implication for protein kinase A, Epac, phosphodiesterases 3 and 4. Cellular Signalling, 2007, 19(1): 81-86.

[41]Ranganathan G, Phan D, Pokrovskava I D, McEwen J E, Li C, Kern P A. The translational regulation of lipoprotein lipase by epinephrine involves an RNA bingding complex including the catalytic subnit of protein kinase A. The Journal of Biological Chemistry, 2002, 277(45): 43281-43287.
[1] WEI JingJie,JIANG NingBo,LIANG Yan,ZHANG Qian,SUN YingJian,HU Ge. Effect of Matrine on NLRP3 Inflammasome Signaling Pathway in H9N2 AIV Infected Mice [J]. Scientia Agricultura Sinica, 2022, 55(21): 4315-4326.
[2] LAN Qun,XIE YingYu,CAO JiaCheng,XUE LiE,CHEN DeJun,RAO YongYong,LIN RuiYi,FANG ShaoMing,XIAO TianFang. Effect and Mechanism of Caffeic Acid Phenethyl Ester Alleviates Oxidative Stress in Liquid Preservation of Boar Semen Via the AMPK/FOXO3a Signaling Pathway [J]. Scientia Agricultura Sinica, 2022, 55(14): 2850-2861.
[3] RAN HongBiao,ZHAO LiLing,WANG Hui,CHAI ZhiXin,WANG JiKun,WANG JiaBo,WU ZhiJuan,ZHONG JinCheng. Effects of lncFAM200B on the Lipid Deposition in Intramuscular Preadipocytes of Yak [J]. Scientia Agricultura Sinica, 2022, 55(13): 2654-2666.
[4] DU Yu,WANG Yong,MENG QingYong,ZHU JiangJiang,LIN YaQiu. Knockdown Goat KLF12 to Promote Subcutaneous Adipocytes Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(1): 184-196.
[5] ZHAO DongMin,HUANG XinMei,ZHANG LiJiao,LIU QingTao,YANG Jing,HAN KaiKai,LIU YuZhuo,LI Yin. The Induction of Unfolded Protein Response in Tembusu Virus Infected Ducklings [J]. Scientia Agricultura Sinica, 2021, 54(4): 855-863.
[6] ZHU JingJing,ZHOU XiaoLong,WANG Han,LI XiangChen,ZHAO AYong,YANG SongBai. Prediction and Verification of MicroRNAs Targeting Porcine Endoplasmic Reticulum Stress Pathway [J]. Scientia Agricultura Sinica, 2020, 53(15): 3169-3179.
[7] GUO HongFang,NING Yue,CHENG Gong,ZAN LinSen. The Effect of Krüppel-Like Factor 3 (KLF3) Gene on Bovine Fat Deposition [J]. Scientia Agricultura Sinica, 2019, 52(7): 1272-1281.
[8] JIN MEI,ZHANG LIJUAN,CAO QIAN,GUO XinYing. The Screening and Identification of LncRNA Related to Villus Growth in Liaoning Cashmere Goats by MT and FGF5 [J]. Scientia Agricultura Sinica, 2019, 52(4): 738-754.
[9] ZHU JiangJiang,LIN YaQiu,WANG Yong,LIN Sen. Expression Profile and Correlations of Kruppel Like Factors During Caprine (Capra Hircus) Preadipocyte Differentiation [J]. Scientia Agricultura Sinica, 2019, 52(13): 2341-2351.
[10] XiaoXia CUI, HongMei SHU, Lu JIANG, XiaoLan HE, YuanYong GONG, WanChao NI, ShuQiao GUO. Identification of Pathogens Causing Brown Spot and the Role of MeJA in Disease Resistance in Stevia rebaudiana [J]. Scientia Agricultura Sinica, 2018, 51(18): 3520-3530.
[11] YUAN XueMei, WANG Min, ZANG JinPing, CAO HongZhe, ZHANG Kang, ZHANG Jing, XING JiHong, DONG JinGao . Relationship between kynurenine 3-monooxygenase gene BcKMO and cAMP signaling pathway in Botrytis cinerea [J]. Scientia Agricultura Sinica, 2018, 51(13): 2504-2512.
[12] YAN XiaoRu, SHI Tao, PAN YangYang, JING JiongJie, CHENG LiFen, CAO NingXian, QIAO LiYing, LIU WenZhong. Regulation of the BCKDHB Gene Expression by miR-433-3p in Ovine Preadipocytes [J]. Scientia Agricultura Sinica, 2017, 50(22): 4389-4397.
[13] ZHANG Min, ZHU Ming, LI WenZong, MA Jie, LIU YuePing, JIANG HaiYang, WANG Lei, XU MiaoYun. Molecular Regulation Mechanism of Leaf Development Mediated by Ath-miR169d in Arabidopsis thaliana [J]. Scientia Agricultura Sinica, 2017, 50(16): 3063-3070.
[14] DOU YiNing, QIN YuHai, MIN DongHong, ZHANG XiaoHong, WANG ErHui, DIAO XianMin, JIA GuanQing, XU ZhaoShi, LI LianCheng, MA YouZhi, CHEN Ming. Transcription Factor SiNAC18 Positively Regulates Seed Germination Under Drought Stress Through ABA Signaling Pathway in Foxtail Millet (Setaria italic L.) [J]. Scientia Agricultura Sinica, 2017, 50(16): 3071-3081.
[15] LIU YingChao, XIAO JingXiu, TANG Li, ZHENG Yi. Effect of Nitrogen Application Rates on Quercetin and Hesperetin Exuded by Roots in Wheat and Faba Bean Intercropping System [J]. Scientia Agricultura Sinica, 2017, 50(16): 3092-3100.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd