Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1736-1744.doi: 10.3864/j.issn.0578-1752.2013.08.024

• RESEARCH NOTES • Previous Articles    

二斑叶螨乙酰辅酶A羧化酶基因全长cDNA克隆及其生物信息学分析

 LU Juan-Juan , WANG  Jin-Jun, GAO  Xin-Ju, SHEN  Hui-Min   

  1. 1.College of Prataculture, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem Education Ministy/The Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070
    2.Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716
  • Received:2012-09-09 Online:2013-04-15 Published:2013-01-22

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Abstract: 【Objective】The objective of this study is to clone full-length cDNA sequence of the Acetyl-CoA carboxylase (ACCase) gene from Tetranychus urticae and analyze its expression in different strains. 【Method】 The full length cDNA of ACCase gene from T. urticae was cloned by using RT-PCR, and the bioinformatics software was employed to analyze the characteristics of the coded protein. Expression profiles of ACCase gene between susceptible strain (S) and spirodiclofen resistant strain (Sp-R) of T. urticae were performed by the quantitative real-time PCR. 【Result】The full-length cDNA of ACCase gene (GenBank accessin number JX424763) contained 7 068 bp encoding 2 235 amino acids. The putative protein of ACCase gene showed predicted molecular weight of 266.35 kD with a theoretical pI of 6.38. The ACCase contained three function domains, including the biotin carboxylase (BC), the biotin carboxyl carrier protein (BCCP) and the carboxyltransferase (CT). The phylogenetic tree of nine ACCase amino acid sequences from different species were constructed by MEGA5.0．The results showed a close relationship with Pediculus humanus corporis. Quantitative real-time PCR showed that the transcripts of ACCase gene were 2-fold higher in spirodiclofen (Sp-R) resistant strain compared with susceptible strain.【Conclusion】The ACCase gene was cloned from T. urticae by using the RT-PCR. The higher expression of ACCase gene was probably related with the development of resistance to spirodiclofen, which may provide a basis for the management of pesticide resistance of T. urticae.

Key words: Tetranychus urticae (Koch) , spirodeclofen , Acetyl-CoA carboxylase , cDNA cloning , real-time PCR

[1]李隆术. 农业螨类研究进展. 中国农业科学, 1990, 23(1): 22-30.

Li L S. Resent progress in the study on agricultural mites. Scientia Agricultura Sinica, 1990, 23(1): 22-30. (in Chinese)

[2]Van Leeuwen T, Vontas J, Tsagkarakou A, Dermauw W, Tirry L. Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: a review. Insect Biochemistry and Molecular Biology, 2010, 40(8): 563-572.

[3]Stumpf N, Nauen R. Biochemical markers linked to abamectin resistance in Tetranychus urticae (Acari: Tetranychidae). Pesiticide Biochemistry and Physiology, 2002, 72(2): 111-121.

[4]Ay R, Gürkan M O. Resistance to bifenthrin and resistance mechanisms of different strains of the two-spotted spider mite (Tetranychus urticae) from Turkey. Phytoparasitica, 2005, 33(3): 237-244.

[5]涂洪涛, 张金勇, 陈汉杰, 郭小辉, 李元朝. 12种杀螨剂对山楂叶螨防效评价及其对天敌塔六点蓟马的影响. 环境昆虫学报, 2009, 31(3): 213-218.

Tu H T, Zhang J Y, Chen H J, Guo X H, Li Y Z. The effect of 12 acaricides on Tetranychus viennensis and its natural enemy Scolothrips takahashii in apple orchard. Journal of Environmental Entomology, 2009, 31(3): 213-218. (in Chinese)

[6]Sinilnikoval O M, Ginolhac S M. Acetyl-CoA carboxylase α gene and breast cancer susceptibility. Carcinogenesis, 2004, 25(12): 2417-2424.                   

[7]Van Pottelberge S, Van Leeuwen T, Khajehali J, Tirry L. Genetic and biochemical analysis of a laboratory-selected spirodiclofen-resistant strain of Tetranychus urticae Koch (Acari: Tetranychidae). Pest Management Science, 2009, 65(5): 358-366.

[8]Van Leeuwen T, Van Pottelberge S, Tirry L. Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae. Pest Management Science, 2005, 61(5): 499-507.

[9]Rauch N, Nauen R. Spirodiclofen resistance risk assessment in Tetranychus urticae. Pesticide Biochemistry and Physiology, 2003, 74(2): 91-101.

[10]段辛乐, 张志刚, 高新菊, 沈慧敏. 二斑叶螨对甲氰菊酯和螺螨酯的抗性选育及增效剂的增效作用. 植物保护, 2011, 37(5): 106-109.

Duan X L, Zhang Z G, Gao X J, Shen H M. Selection of Tetranychus urticae resistant to fenpropathrin and spirodiclofen and the synergistic action of the synergists to the resistant population. Plant Protection, 2011, 37(5): 106-109. (in Chinese)

[11]符海波, 张志刚, 沈慧敏, 杨顺义. 二斑叶螨对螺螨酯抗性和敏感种群相对适合度的研究. 植物保护, 2011, 37(5): 115-117.

Fu H B, Zhang Z G, Shen H M, Yang S Y. Biological fitness of the spirodiclofen-resistant and sensitive populations of Tetranychus urticae. Plant Protection, 2011, 37(5):115-117. (in Chinese)

[12]Grbic M, Van Leeuwen T, Clark R M, Rombauts S, Rouze P, Grbic V, Osborne E J , Dermauw W, Ngoc P C, Ortego F, Hernandez-Crespo P, Diaz I, Martinez M, Navajas M, Sucena E, Magalhaes S, Nagy L, Pace R M, Djuranovic S, Smagghe G, Iga M, Christiaens O, Veenstra J A, Ewer J, Villalobos R M, Hutter J L, Hudson S D, Velez M, Yi S V, Zeng J, Pires-daSilva A, Roch F, Cazaux M, Navarro M, Zhurov V, Acevedo G, Bjelica A, Fawcett J A, Bonnet E, Martens C, Baele G, Wissler L, Sanchez-Rodriguez A, Tirry L, Blais C, Demeestere K, Henz S R, Gregory T R, Mathieu J, Verdon L, Farinelli L, Schmutz J, Lindquist E, Feyereisen R, Van de Peer Y. The genome of Tetranychus urticae reveals herbivorous pest adaptations. Nature, 2011, 479: 487-492.

[13]高新菊. 二斑叶螨对甲氰菊酯的抗性分子机理研究[D]. 兰州: 甘肃农业大学, 2012.

Gao X J. Research on resistance molecular mechanism of Tetranychus urticae Koch to fenpropathrin[D]. Lanzhou: Gansu Agricultural University, 2012. (in Chinese)

[14]Tong L. Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cellular and Molecular Life Sciences, 2005, 62(16): 1784-1803.

[15]杨雪莹. 不同种类除草剂的作用方式研究及ACCase CT 功能域模型的建立[D]. 长春: 吉林大学, 2008.

Yang X Y. The research of different herbicides’ modes of action and modelling of ACCase CT domain[D]. Changchun: Jilin University, 2008. (in Chinese)

[16]Yang X, Guschina I A, Hurst S, Wood S, Langford M, Hawkes T, Harwood J L. The action of herbicides on fatty acid biosynthesis and elongation in barley and cucumber. Pest Management Dcience, 2010, 66(7): 794-800.

[17]Tal A, Rubin B. Molecular characterization and inheritance of resistance to ACCase-inhibiting herbicides in Lolium rigidum. Pest Management Science, 2004, 60: 1013-1018.

[18]Focks N, Benning C. Wrinkled1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiology, 1998, 118: 91-101.

[19]楚敏, 赵虎基, 郑明刚, 刘红玲, 乐锦华. 谷子乙酰辅酶A羧化酶BC功能域的克隆及原核表达载体的构建. 石河子大学学报: 自然科学版, 2004, 22(5): 408-410.

Chu M, Zhao H J, Zheng M G, Liu H L, Le J H. Cloning and construction of prokaryotic expression vector of BC subunit from Acetyl-coA carboxylase in mille. Journal of Shihezi University: Natural Science, 2004, 22(5): 408-410. (in Chinese)

[20]Ohlrogge J B, Roesler K R, Shorrosh B S. Methods of Increasing Oil Content of Seeds. United States Patent, 1999: 5925805.

[21]Sellwood C, Slabas A R, Rawsthorne S. Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos. Biochemical Society Transactions, 2000, 28: 598-600.

[22]Bretschneider T, Fischer R, Nauen R. Inhibitors of lipid     synthesis (Acetyl-CoA-carboxylase inhibitors)//Krämer W, Schirmer U. Modern Crop Protection Compounds. Wiley, Weinheim, 2007: 909-925.

[23]Dekeyser M A. Acaricide mode of action. Pest Maagement Science, 2005, 61: 103-110.

[24]Wachendorff U, Nauen R, Schnorbach H J, Rauch N, Elbert A.    The biological profile of spirodiclofen (Envidor®)-A new selective tetronic acid acaricide. Pflanzenschutz-Nachrichten Bayer, 2002, 55: 149-176.
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