Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (8): 1725-1735.doi: 10.3864/j.issn.0578-1752.2013.08.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of Full-Length cDNA Sequence of YAP1 Gene in Sheep

 SUN  Wei, LI  Da, SU  Rui, MA  Yue-Hui, GUAN  Wei-Jun, ZHANG  You-Fa, CHEN  Ling, WU  Wen-Zhong, ZHOU  Hong   

  1. 1.Animal Science and Technology College, Yangzhou University, Yangzhou 225009, Jiangsu
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193
    3.Animal Science and Veterinary Medicine Bureau of Suzhou, Suzhou 215128, Jiangsu
    4.Forestation, Herding, Fishing Bureau of Suining Country of Xuzhou, Suining 221200, Jiangsu
  • Received:2012-12-24 Online:2013-04-15 Published:2013-03-08

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Abstract: 【Objective】 There was no report on YAP1 gene and its protein in the past days, this study was carried out in order to clone the YAP1 cDNA in sheep and provide a basic foundation for the study of YAP1 genetic characters , as well as analyze its tissue different level expression patterns. 【Method】 Hu sheep was regarded as the research object, and the full-length cDNA sequence of the YAP1 gene was acquired from the longissimus dorsi muscle by RT-PCR and RACE, and the sequence was analyzed in depth by bioinformatics method. 【Result】 The full-length cDNA sequence of the YAP1 gene was successfully obtained from Hu sheep longissimus dorsi muscle. Bioinformatics technology was successfully used to analyze the YAP1 homology among different species, transmembrane region of YAP1 sequence, subcellular localization, hydrophilic, potential signal peptide cleavage sites, glycosylation sites, phosphorylationlocus, product function prediction analyisis, YAP1 secondary structure and teriary structure.【Conclusion】The full-length of YAP1 gene was 1 712bp, including 1 212bp coding sequence encoding 403 amino acids. The homology analysis found that the nucleotide sequence and amino acid sequence of sheep YAP1 gene share the highest 78.77% and 92.51% identity with the Cricetulus YAP1 while there was no large difference between Human and Chimpanzee. The amino acid sequence analysis revealed that the YAP1 gene of sheep encoded water-soluble protein and its relative molecular weight was 44079.0 Da, isoelectric point was 4.91, was a kind of hydrophilic non-transmembrane protein. Subcellular localization of YAP1 was in the mucleus, and it did not belong to the secreted protein. The YAP1 protein contained 33 phosphorylation sites, 7 glycosylation sites and 2 WW domain. The secondary structure of YAP1 was mainly composed of random coil. The tertiary structure of domain area of YAP1 protein showed a forniciform helix structure. Forecast YAP1 had a key role in regulatory functions process. The above studies have laid an information foundation for further study of the YAP1 gene function in the sheep muscle development process.

Key words: YAP1 , clone , bioinformatics analysis , expression

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