Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 205-214.doi: 10.3864/j.issn.0578-1752.2013.01.024

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Expression Analysis of a BnICE1 from Brassica napus L.

 ZHANG  Teng-Guo, CHANG  Yan, WANG  Juan, WANG  Ning, WANG  Yuan-Yuan, CHEN  Qiong-Qiong, SUN  Wan-Cang   

  1. 1.School of Life Sciences, Northwest Normal University, Lanzhou 730070
    2.College of Agronomy, Gansu Agricultural University, Lanzhou 730070
  • Received:2012-09-19 Online:2013-01-01 Published:2012-11-15

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Abstract: 【Objective】 To study the function of BnICE1 in response to low temperature, transcription factor BnICE1 which is involved in cold stress responsiveness in Brassica napus was cloned and its sequence was analyzed. 【Method】 The full-length cDNA of BnICE1 was obtained by the technology of rapid amplification of cDNA ends (RACE). The cDNA sequence and the deduced amino acid sequence were analyzed by bioinformatics method. The spatial-specific expression of BnICE1 in different tissues of the control plants and the expression patterns of BnICE1 in leaves under cold stress condition were determined by real-time RT-PCR. 【Result】In this study, a novel gene, BnICE1, from B. napus was isolated and characterized. The full-length cDNA was 1 737 bp, containing a 1 500 bp opening reading frame(ORF). The deduced protein was 499 amino acids with molecular weight 53.2 kD and isoelectric point 5.0. The sequence aligment demonstrated that the C-termina1 of deduced BnICEI protein exhibited a typical bHLH domain and was high identity with other ICE1 proteins from other species. So it was named the BnICE1 (GenBank accession No. JF268687). The result of phylogenetic tree showed that BnICE1 was closer to BcICE1 and BchICE1. Analysis by real-time RT-PCR indicated that BnICE1 was expressed in different tissues (stem, leaf, hypocotyls) and that relatively higher abundance was observed in hypocotyls. 【Conclusion】 The transcription factor BnICE1 was cloned from B. napus and the analysis by real-time RT-PCR suggested that BnICE1 played an important role in defending cold stress.

Key words: Brassica napus L. , BnICE1 , molecular cloning , expression analysis

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