Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (23): 4909-4915.doi: 10.3864/j.issn.0578-1752.2012.23.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Royal Jelly on the DNA Methylation of dynactin p62 in Female Honeybee Larvae

 SHI  Yuan-Yuan, TIAN  Liu-Qing, ZHANG  Fei, LIU  Jun-Feng, YAN  Wei-Yu, ZENG  Zhi-Jiang   

  1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045
  • Received:2012-04-26 Online:2012-12-01 Published:2012-07-02

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Abstract: 【Objective】 The objective of this study is to analyze the influence of royal jelly on DNA methylation of dynactin p62 in female honeybee larvae. 【Method】 One-day-old larvae of Apis mellifera ligustica and A. cerana cerana were used as materials, they were fed with royal jelly either from A. mellifera (RJM) or from A. cerana (RJC), and genome DNA was extracted from 3-and 6-day-old larvae, then DNA methylation of dynactin p62 was detected by Sequenom MassARRAY. 【Result】 The whole DNA methylation of dynactin p62 in female honeybee larvae decreased significantly after feeding with heterospecific royal jelly. The whole DNA methylation of dynactin p62 in 6-day-old larvae was significantly lower than 3-day-old larvae after feeding with homologous royal jelly. The methylated sites of dynactin p62 in larvae were different after feeding with royal jelly (RJM and RJC). 【Conclusion】 Royal jelly (RJM and RJC) had different effects on the whole DNA methylated and methylated sites of dynactin p62 in female honeybee larvae. These results indicate that different types of royal jelly have different biological effects.

Key words: honey bee , royal jelly , dynactin p62 , DNA methylation

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