Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3608-3616.doi: 10.3864/j.issn.0578-1752.2012.17.018

• VETERINARY SCIENCE • Previous Articles     Next Articles

Antibiotic Resistance and Coagulase Typing of Staphylococcus aureus Isolates from Pigs and Cows in Part of China

 LIU  Yang, LIANG  Yao-Feng, JIAO  Xin-An, SONG  Li, ZHANG  Chun-Ping, NING  Yi-Bao, XU  Shi-Xin   

  1. 1.扬州大学兽医学院，江苏扬州 225009
    2.中国兽医药品监察所, 北京 100081；3华南农业大学，广州 510642
  • Received:2012-03-09 Online:2012-09-01 Published:2012-07-26

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Abstract: 【Objective】The objective of the experiment is to study the status of antibiotics resistance and coagulase typing distribution of S.aureus in China, and provide a scientific basis for effective control of transmission and prevalence of S.aureus from animals.【Method】Antibiotics resistance was tested by broth dilution method, coagulase serotypes were identified by coagulase grouping serum, coa genes were amplified by PCR method and then sequenced for further analysis by mega software. 【Result】 A total of 124 S.aureus isolates were identified, and the result of resistance detection revealed that the resistant rate of ampicillin and penicillin was near 100%, erythromycin reached 75%, and other 10 antibiotics was below 50%. The positive rate of MRSA was 27%. No vancomycin resistant isolates was found. Six kinds of serotypes were observed and the rate of coagulase serotyping reached 91%: serotypes VII,VI and combined type were prevalent, whereas serotypes Ⅲ and Ⅴwere not found. The phylogenetic tree of coagulase gene was constructed. 【Conclusion】 The status of antibiotics resistance in China was relatively severe, swine isolates were severer than cattle ones. Coagulase serotypes were abundant and the distribution was comparative intensive, and possessed unique prevalence feature. The evolutional characteristic of coa gene had no direct relationship with coagulase serotypes, and this study has provided a theoretical basis for further veterinary research about molecular epidemiology and evolutionary tendency.

Key words: S. aureus, antibiotics resistance, coagulase typing, pig, cow  

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