Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3515-3521.doi: 10.3864/j.issn.0578-1752.2012.17.007

• PLANT PROTECTION • Previous Articles     Next Articles

Identification and rDNA-ITS Sequence Analysis of the Pathogen of Kenaf Anthracnose

 LIU  Xiao-Qian, QI  Jian-Min, CHEN  Yu-Sen, CHEN  Mian-Cai, CHEN  Mei-Xia, LIU  Wei, FANG  Ping-Ping, LIN  Li-Hui, TAO  Ai-Fen   

  1. 1.福建农林大学作物遗传育种与综合利用教育部重点实验室/农业部东南黄/红麻科学观测实验站，福州 350002
    2.福建农林大学植物保护学院，福州 350002；3海南省农业科学院农业环境与植物保护研究所，海口 570100
  • Received:2012-01-04 Online:2012-09-01 Published:2012-07-31

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Abstract: 【Objective】The objective of this study is to determine the possible pathogens of kenaf anthracnose of China.【Method】The samples of diseased kenaf were collected and isolated from Anhui, Henan, Zhejiang, Fujian Provinces of China. AH, HN, ZJ, FJ strains were selected from sixteen pathogens and their pathogenicities were determined. Identification was carried out by using traditional morphological methods and sequencing of rDNA-ITS. 【Result】 The differences among AH, HN, ZJ and FJ strains in morphological characteristics and pathogenicities were relatively abundant. The rDNA-ITS length of AH, HN, ZJ, and FJ strains was 541, 545, 541, and 535 bp, respectively. The homology between FJ strain and other strains was 90%-91%, and the homology among AH, HN, ZJ strains was 96%-98%.【Conclusion】The pathogens of kenaf anthracnose were identified by analysis at the molecular level. It is confirmed that kenaf anthracnose in China is caused by C. dematium and C. gioeosporioides, and the pathogenicity of C. dematium is greater than C. gioeosporioides.

Key words: kenaf, Colletotrichum, pathogen species, pathogenicity, rDNA-ITS sequence

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