Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3346-3356.doi: 10.3864/j.issn.0578-1752.2012.16.014

• HORTICULTURE • Previous Articles     Next Articles

Degradation of Plastid Pigment and Its Relationship with Volatile Catabolite Content in Cured Leaves of Different Genotypes of Flue-Cured Tobacco

 SHI  Hong-Zhi, GU  Shao-Long, DUAN  Wei-Dong, WANG  Jian-An, QIAN  Hua, LIU  Qing-Hua, SU  Fei, LIU  Guo-Shun   

  1. 1.河南农业大学烟草学院,郑州 450002
    2.河南中烟工业公司,郑州 450000
    3.平顶山烟草公司,河南平顶山 467000
  • Received:2011-11-16 Online:2012-08-15 Published:2012-06-12

Abstract: 【Objective】 The research was conducted to investigate the changes in plastid pigment contents during middle leaf mature and curing, and the relationship of pigment degradation amount and rate to volatile pigment catabolite content in cured leaves. 【Method】HPLC method was used to measure the pigment and volatile aroma components in 9 genotypes of flue-cured tobacco grown in central Henan Province. 【Result】The results showed that the plastid pigment contents decreased during leaf maturing and curing, with the total degradation amount and rate of chlorophyll being greater than that of carotenoids, and that of chlorophyll a being significantly greater than that of chlorophyll b, resulting in relatively higher amount of residue of chlorophyll b in cured leaves. The degradation rate of chlorophyll during leaf maturing was greater than that during curing. Among the carotenoids, lutein had the highest content and greatest degradation amount, and that during leaf maturing was greater than that during curing. The degradation rate of neoxanthin during maturing was also greater that during curing, while it was the contrary for β-carotene and violaxanthin. Different genotypes had different pigment degradation amounts and had significantly positive correlations with the contents of volatile degraded aroma compounds in cured leaves, while there was no or negative correlations with the contents of pigment residue in cured leaves. Total amount of pigment-degraded aroma compounds and the contents of most important individual aroma components had greater positive correlations with degradation amount during maturing than that during curing. 【Conclusion】 The degradation amount of chlorophyll, luten and neoxanthin were greater than that during curing. Total amount of pigment degraded aroma compounds had a close relationship with the pigment degradation amount, especially that during maturing. Thus the increase of maturity of tobacco was pivotal in promoting the formation of aroma components in tobacco leaves.

Key words: flue-cured tobacco, genotype, plastid pigment, degradation amount, catabolite

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