Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (15): 3203-3212.doi: 10.3864/j.issn.0578-1752.2012.15.024

• RESEARCH NOTES • Previous Articles     Next Articles

High-Molecular-Weight Glutenin Subunit Composition of Chinese Wheat Germplasm with Desirable Agronomic Traits

 YAN  Yuan-Yuan, GUO  Xiao-Min, LIU  Wei-Hua, GAO  Ai-Nong, LI  Xiu-Quan, YANG  Xin-Ming, CHE  Yong-He, LI  Li-Hui   

  1. 1.河北科技师范学院生命科技学院，河北秦皇岛 066004
    2.中国农业科学院作物科学研究所，北京 100081
  • Received:2012-03-07 Online:2012-08-01 Published:2012-04-25

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Abstract: 【Objective】 The purpose of this research was to screen wheat (Triticum aestivum L.) germplasm with many excellent properties and provide useful inforrnation for the effective use. 【Method】High molecular weight glutenin subunits (HMW-GS) compositions of 403 superior germplasm recently developed or introduced in China with desirable agronomic traits were detected using sodium dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE). 【Result】The results indicated that a high level of variations in HMW-GS encoded by Glu-1 locus were observed. Thirty allele variations with 64 kinds of glutenin patterns were found. Meanwhile, there were not only rare subunits “7*+9”, “2+12.2*”, “1.5*+10”, etc., but also special form of subunit combinations in modern wheat breeding materials “1+2*”, “7+8+9”, “6+7+8+9”, “2+5+12”, etc., were showed. The percentage of materials with two elite subunits was 24.32% (98 varieties). The percentage of germplasm with desirable subunits to wheat processing quality in all three locus (Glu-A1, Glu-B1 and Glu-D1) was 9.93% (40 varieties). 【Conclusion】Thirty allele variations with 64 kinds of glutenin patterns in HMW-GS encoded by Glu-1 locus of the 403 superior germplasms were detected. Forty germplasms bring desirable subunits to wheat processing quality in all three locus (Glu-A1, Glu-B1 and Glu-D1) were sought out. The newly developed germplasm with desirable agronomical traits and HMW-GS for good quality would be used for the improvement of wheat quality breeding.

Key words: common wheat, agronomic trait, HMW-GS, superior germplasm

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