Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4882-4891.doi: 10.3864/j.issn.0578-1752.2011.23.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effects of Dietary Synbiotics on Intestinal Microbial Ecology in Broiler Chickens

 YANG  Hui-Ling, GAO  Yu-Peng, ZHOU  Li-Yong, XU  Yao-Xing, LI  Yan-Li, XU  Shao-Chun   

  1. 1.西北农林科技大学动物科技学院，陕西杨凌 712100
    2.浙江省农业科学院植物保护与微生物研究所，杭州 310021
  • Received:2011-07-01 Online:2011-12-01 Published:2011-10-18

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Abstract: 【Objective】 This study was carried out to investigate the effects of dietary supplementation of synbiotics on intestinal microbial flora ecology in broilers.【Method】Four hundred and fifty 1-d-old Cobb 48 broiler chicks were randomly assigned into 5 dietary treatments, each treatment allocated to 3 replicates of 30 broilers. The dietary treatments were control, basal diets supplemented with antibiotics, basal diets supplemented with probiotics, basal diets supplemented with prebiotics, and basal diets supplemented with synbiotics. On day 21 and 42, four birds from each replicate were randomly selected and killed, cecal contents were aseptically collected, and the total genomic DNA was extracted, then the intestinal bacterial community was analyzed by denaturing gradient gel electrophoresis (DGGE) analysis of universal 16S rDNA after amplication with PCR, and the populations of total bacteria, Lactobacillus, Bifidobacterium and E.coil were detected by real-time PCR. 【Result】 On day 21 and 42, the supplementation of synbiotics increased average daily gain and feed conversion ratio, compared with prebiotics, probiotics or antibiotics alone (P＜0.05), and the number of PCR-denaturing gradient gel electrophoresis bands of synbiotics-fed group was higher than those in the control, antibiotics, probiotics and prebiotics groups. Gene sequences analysis indicated that the specific bands were mainly uncultured bacteria in cecum of broilers. Dietary synbiotics increased the populations of total bacteria, Lactobacillus, Bifidobacterium and E.coil.【Conclusion】The effects of synbiotics on growth performance was consistent with that on intestinal microbial ecology, and the effects of synbiotics was better than probiotics, prebiotics or antibiotics alone.

Key words: synbiotics, broiler, intestinal microflora, denaturing gradient gel electrophoresis, real-time PCR

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