日粮氮水平对牦牛瘤胃发酵参数和氮组分的影响

doi:10.3864/j.issn.0578-1752.2011.22.015

Abstract

Abstract: 【Objective】 The objective of this study is to evaluate the effect of different N levels of diets on the ruminal fermentation and nitrogen fractions in yak. 【Method】 Four castrated yaks at (148.5±9.2) kg and 3-year-old of age were used in a partially replicated 4×4 Latin square with 4 different N levels of diets treatments (A(35.92 g•d-1), B(49.44 g•d-1), C(61.28 g•d-1), D (72.48 g•d-1)) and 4 20-day periods. pH, FAA-N (free amino acid nitrogen), NH3-N (NH3 nitrogen), PAA-N (peptide amino acid nitrogen), soluble protein N (nitrogen) and VFA (volatile fatty acid) were studied in different times before (07：30) and after feeding (10：00, 12：00, 14：00, 16：00) at last day. 【Result】 Ruminal pH was not affected by diets (P＞0.05). PAA-N, soluble protein N, FAA-N, total VFA and NH3-N were significantly differed among yaks with different diets (P＜0.05). NH3-N was also affected by diet×time interaction (P＜0.05). Acetate/propionate was not affected by different diets (P＞0.05). 【Conclusion】 Under the rations of the present study, when N of diet greater than 1.97%, most nitrogen fractions in yak do not response to the nitrogen level of the experimental diets. At 2.90% nitrogen of diet, acetate/propionate is the minimum and total VFA is the maximum.

Key words: yak, N level, rumen, N fraction

MI Jian-Dui, GUO Xu-Sheng, ZHOU Jian-Wei, XIN Guo-Sheng, ZHANG Ying, ZHU Yu-Huan, LONG Rui-Jun. Effect of Nitrogen Levels of Diets on Ruminal Fermentation Characters and Nitrogen Fractions in Yak[J].Scientia Agricultura Sinica, 2011, 44(22): 4678-4686.

References

[1]Tajima K, Aminov R I, Nagamin T, Matsui H, Nakamura M, Benno Y. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Applied and Environmental Microbiology, 2001, 67(6): 2766-2774.

[2]Sylvester J T, Karnati S K, Dehority B A, Morrison M, Smith G L, St-Pierre N R, Firkins J L. Rumen ciliated protozoa decrease generation time and adjust 18S ribosomal DNA copies to adapt to decreased transfer interval, starvation, and monensin. Journal of Dairy Science, 2009, 92(1): 256-269.

[3]刁其玉. 动物氨基酸营养与饲料. 北京: 化学工业出版社, 2007.

Diao Q Y. Amino Acid Nutrition and Forage of Animal. Beijing: Chemistry Industry Press, 2007. (in Chinese)

[4]Wang M P, Zhao C Z, Long R J, Yang Y H. Rangeland governance in China: overview, impacts no Sunan county in Gansu province and future options. The Rangeland Journal, 2010, 32: 155-163.

[5]王文娟, 汪水平, 谭支良. 反刍动物瘤胃氮代谢研究进展. 华中农业大学学报, 2007, 26: 747-754.

Wang W J, Wang S P, Tan Z L. Research advance on nitrogen metabolism in the rumen of ruminants. Journal of Huazhong Agricultural University, 2007, 26: 747-754. (in Chinese)

[6]冯仰廉. 反刍动物营养学. 北京: 科学出版社, 2004.

Feng Y L. Animal Nutrition of Ruminant. Beijing: Science Press, 2004. (in Chinese)

[7]董世魁, 龙瑞军, 胡自治. 不同采食水平下舍饲干奶牦牛能量转化、氮、钙、磷代谢的研究. 草业学报, 2000, 2: 32-37.

Dong S K, Long R J, Hu Z Z. A study on conversion of energy and metabolism of protein, calcium and phosphorus in dry yaks. Acta Prataculturae Sinica, 2000, 2: 32-37. (in Chinese)

[8]韩兴泰, 胡令浩, 谢敖云. 粗饲条件下生长牦牛能量代谢的估测. 青海畜牧兽医杂志, 1992, 2: 21-22.

Han X T, Hu L H, Xie A Y. Evaluation of energy metabolism for growing yak under roughage condition. Chinese Qinghai Journal of Animal and Veterinary, 1992, 2: 21-22. (in Chinese)

[9]胡令浩, 谢敖云, 韩兴泰. 生长牦牛与生长黄牛体表面积的研究. 中国畜牧杂志, 1994, 6: 9-10.

Hu L H, Xie A Y, Han X T. Study on the surface areas of growing yak and cattle. Chinese Journal of Animal Science, 1994, 6: 9-10. (in Chinese)

[10]薛白, 韩兴泰. 日粮精料水平对牦牛和黑白花牛营养物质利用率的影响. 中国草食动物, 2001, 3: 3-8.

Xue B, Han X T. The influence of dietary concentrate level on nutrients utilization of Holstein, yak. China Herbivores, 2001, 3: 3-8. (in Chinese)

[11]吕秉林, 扎西卓玛. 牦牛与黑白花牛的氮平衡比较研究. 黄牛杂志, 2002, 28: 17-18.

Lü B L, Zhaxi Z M. Comparison of nitrogen balance in yak and Holstein. Journal of Yellow Cattle Science, 2002, 28: 17-18. (in Chinese)

[12]龙瑞军, 董世魁, 胡自治. 同一日粮下泌乳牦牛与干奶牦牛消化代谢能力的比较研究. 草业学报, 1998, 7: 51-55.

Long R J, Dong S K, Hu Z Z, Nutrient digestion and metabolism in lactating and dry yak cows. Acta Prataculturae Sinica, 1998, 7: 51-55. (in Chinese)

[13]Long R J, Dong S K, Chen X B, Orskov, Hu Z Z. Preliminary studies on urinary excretion of purine derivatives and creatinine in yaks. The Journal of Agricultural Science, 1999, 133: 427-431.

[14]Long R J, Dong S K, Hu Z Z, Shi J J, Dong Q M, Han X T. Digestibility, nutrient balance and urinary purine derivative excretion in dry yak cows fed oat hay at different levels of intake. Livestock Production Science, 2004, 88: 27-32.

[15]Long R J, Dong S K, Wei X H, Pu X P. The effect of supplementary feeds on the bodyweight of yaks in cold season. Livestock Production Science, 2005, 129: 133-137.

[16]Wang H C, Long R J, Zhou W, Li X P, Zhou J W, Guo X S. A comparative study on urinary purine derivative excretion for yak(Bos grunniens), indigenous cattle(Bos taurus) and crossbred(Bos grunniens × Bos taurus) in Qing-hai Tibetan Plateau, China. Journal of Animal Science, 2009, 87: 2355-2362.

[17]谢敖云, 刘书杰, 韩兴泰, 胡令浩. 不同营养水平对牦牛瘤胃内VFA的影响. 青海畜牧兽医杂志, 1992, 22: 5-7.

Xie A Y, Liu S J, Han X T, Hu L H. Effect of different nutritive levels on rumen VFA for Chinese Yak. Chinese Qinghai Journal of Animal and Veterinary, 1992, 22: 5-7. (in Chinese)

[18]胡令浩. 中国牦牛营养研究进展(二)-生长期牦牛的氮代谢. 青海科技, 2001, 6: 37-39.

Hu L H. Nutrition of yak in China progress(second)-nitrogen metabolism of growing yak. Qinghai Technology, 2001, 6: 37-39. (in Chinese)

[19]胡令浩. 谢敖云, 刘书杰. 生长期牦牛的氮代谢及补氮技术研究. 动物营养学报, 1999, 11: 208-216.

Hu L H, Xie A Y, Liu S J. Study on nitrogen metabolism and nitrogen supplementary techniques in growing yaks. Acta Zoonutrometa Sinica, 1999, 11: 208-216. (in Chinese)

[20]薛白, 韩兴泰. 牦牛瘤胃内饲料蛋白质降解率的研究. 动物营养学报, 1998, 10: 35-39.

Xue B, Han X T. Protein degradation of feedstuffs in the rumen of yaks. Acta Zoonutrometa Sinica, 1998, 10: 35-39. (in Chinese)

[21]胡令浩, 中国牦牛营养研究进展(一)-瘤胃消化代谢与能量代谢. 青海科技, 2001, 5: 40-42.

Hu L H. Nutrition of yak in China progress(first)-digestion and energy metabolism in rumen. Qinghai Technology, 2001, 5: 40-42. (in Chinese)

[22]Chizzotti M L, Valadares S C, Tedeschi L O, Chizzotti F H M, Carstens G E. Energy and protein requirements for growth and maintenance of F1 Nellore×Red Angus Bulls, steers, and heifers. Journal of Animal Science, 2007, 85: 1971-1981.

[23]Broderick G A, Kang J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 1980, 63(1): 64-75.

[24]Fu C J, Felton E E, Lehmkuhler J W, Kerley M S. Ruminal peptide concentration required to optimize microbial growth and efficiency. Journal of Animal Science, 2001, 79(5): 1305-1312.

[25]张丽英. 饲料分析及饲料质量检测技术. 第二版. 北京: 中国农业出版社, 2003.

Zhang L Y. Feed Analysis and Determining Technique. 2 ed. Beijing: Chinese Agricultural University Press, 2003. (in Chinese)

[26]杨胜. 饲料分析及饲料质量检测技术. 北京: 中国农业大学出版社, 1993.

Yang S. Feed Analysis and Determining Technique. Beijing: China Agricultural University Press, 1993. (in Chinese)

[27]Erwin E S, Marco G J, Emery E M. Volatile fatty acid analyses of food and rumen fluid by gas chromatography. Journal of Dairy Science, 1961, 44: 1768-1771.

[28]Licitra G, Hernandez T M, van Soest P J. Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science Technology, 1996, 57: 347-358.

[29]Chen X B, Gomes M J. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives-an overview of the technical detail. Bucksburn Aberdeen: International Feed Resources Unit, 1992.

[30]Lyle R R, Johnson R R, Wilhite J V, Backus W R. Ruminal characteristics in steers as affected by adaptation from forage to all-concentrate diets. Journal of Animal Science, 1981, 53: 1383-1390.

[31]Towne G, Nagaraja T G, Brandt R T, Kemp K E. Dynamics of ruminal ciliated protozoa in feedlot cattle. Applied and Environmental Microbiology, 1990, 56: 3174-3178.

[32]Slyter L L, Oltjen R R, Kern D L, Blank F C. In?uence of type and level of grain and diethylstilbestrol on the rumen microbial populations of steers fed all-concentrate diets. Journal of Animal Science, 1970, 31: 996-1002.

[33]Towne G, Nagaraja T G, Brandt R T, Kemp K E. Ruminal ciliated protozoa in cattle fed ?nishing diets with or without supplemental fat. Journal of Animal Science, 1990, 68: 2150-2155.

[34]Franzolin R, Dehority B A. Effect of prolonged high-concentrate feeding on ruminal protozoa concentrations. Journal of Animal Science, 1996, 74: 2803-2809.

[35]Reynal S M, Ipharraguerre I R, Liñeiro M, Brito A F, Broderick G A, Clark J H. Omasal flow of soluble proteins, peptides, and free amino acids in dairy cows fed diets supplemented with proteins of varying ruminal degradabilities. Journal of Dairy Science, 2007, 90(4): 1887-1903.

[36]Hristov A N, Ivan M, Rode L M, McAllister T A. Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium- or high-concentrate barley-based diets. Journal of Animal Science, 2001, 79(2): 515-524.

[37]韩兴泰, 陈杰, 韩正康. 饲喂不同蛋白质水平日粮的牦牛六位氮代谢与十二指肠各氮组分流量. 动物营养学报, 1998, 10(1): 34-43.

Han X T, Chen J, Han Z K. Ruminal nitrogen metabolism and the flows of nitrogen fractions reaching the duodenum of growing yaks fed diets containing different level of crude protein. Acta Zoonutrometa Sinica, 1998, 10(1): 34-43. (in Chinese)

[38]Patra A K. Effects of supplementing low-quality roughages with tree foliages on digestibility, nitrogen utilization and rumen characteristics in sheep: a meta-analysis. Journal of Animal Physiology and Animal Nutrition, 2010, 94(3): 338-353.

[39]Fox D G, Tedeschi L O, Tylutki T P, Russell J B, van Amburgh M E, Chase L E, Pell A N, Overton T R. The Cornell net carbohydrate and protein system model for evaluating herd nutrition and nutrient excretion. Animal Feed Science and Technology, 2004, 112: 29-78.

[40]Russell J B, Connor J D, Fox D G, Van Soest P J, Sniffen C J. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science, 1992, 70(11): 3551-3561.

[41]Sniffen C J, Connor J D, Van Soest P J, Fox D G, Russell J B. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. Journal of Animal Science, 1992, 70(11): 3562-3577.

[42]Licitra G, Hernandez T M, van Soest P J. Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 1996, 57(4): 347-358.

[43]Preston T R, Leng K A. Matching ruminant production system with available resources in the tropics and sub-tropics. Armidale, NSW: Penambul Books, 1987.

[44]Chen G, Sniffen C J, Russell J B. Concentration and estimated flow of peptides from the rumen of dairy cattle: effects of protein quantity protein solubility and feeding frequency. Journal of Dairy Science, 1987, 70: 983-992.

[45]Kim H D, Kim S W, Kim W Y, Ko Y K, Itabashi H. Effects of popped soybean on concentration of ruminal peptide and blood amino acids in Holstein calves. Asian-Australasian Journal of Animal Sciences, 1998, 11(2): 155-161.

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Effect of Nitrogen Levels of Diets on Ruminal Fermentation Characters and Nitrogen Fractions in Yak

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