Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 3937-3944.doi: 10.3864/j.issn.0578-1752.2011.19.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

The Relationship on Anther Tapetum, Sporopollenin and Expression of RAFTIN1 in Physiological Male Sterile Wheat

 SHENG  Ying, ZHANG  Gai-Sheng, LI  Ya-Xin, ZHANG  Long-Yu, WANG  Shu-Ping, ZHAO  Xin-Liang, WANG  Liang-Ming, SONG  Yu-Long   

  1. 1.西北农林科技大学农学院/陕西省作物杂种优势研究与利用重点实验室/小麦育种教育部工程研究中心
  • Received:2011-03-04 Online:2011-10-01 Published:2011-05-13

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Abstract: 【Objective】 To pave the way for revealing the mechanism of physiological male sterility in wheat, the relevance among expression of RAFTIN1, anther tapetum and sporopollenin were studied.【Method】Using physiologically male sterility induced with chemical hybridizing agent SQ­1, genetically male sterility and corresponding normal lines of complete fertility as experimental materials in wheat, morphological changes of anther tapetum from different developmental stages of microspore, sporopollenin accumulation and RAFTIN1 expression were analyzed via paraffin section, cell fluorescence staining and Real-time PCR. 【Result】The results showed that anther tapetum of physiological male sterile lines pre-degraded, sporopollenin secretion decreased, while RAFTIN1 expression level was maximum at mononuclear stage, and contributed to a large number of sporopollenin to pollen intine. At binuclear and trinuclear stages, the tapeta of physiological male sterility were degraded completely, sporopollenin secretion was ceased and expression patterns of RAFTIN1 were down-regulated significantly. 【Conclusion】 These data demonstrated that the mechanisms of physiologically male sterility induced by SQ­1 were associated with pre-degradation of tapetum and expression degree of RAFTIN1 with responsibility for transporting sporopollenin.

Key words:  wheat, chemical hybridizing agent SQ­1, anther tapetum, sporopollenin, RAFTIN1 gene, real-time PCR

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