Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (2): 341-348 .doi: 10.3864/j.issn.0578-1752.sas-2010-06454

• HORTICULTURE • Previous Articles     Next Articles

Screening Potential DNA Barcode Regions of Chloroplast Coding Genome for Citrus and Its Related Genera

YU Jie, YAN Hua-xue, LU Zhen-hua, ZHOU Zhi-qin
   

  1. (西南大学南方山地园艺学教育部重点实验室)
  • Received:2010-04-20 Revised:2010-10-13 Online:2011-01-15 Published:2011-01-15
  • Contact: ZHOU Zhi-qin

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Abstract:

【Objective】 Four coding regions of chloroplast genome of Citrus and its close relatives were analyzed in an attempt to find suitable DNA barcoding markers for species identification and lay a foundation for further study of non-coding region.【Method】 Four chloroplast DNA regions (matK, rpoB, rpoC1 and rbcL ) of 59 Citrus accessions were sequenced, the intergeneric, interspecific, intraspecific genetic distances were calculated, and the phylogenetic tree of all the accessions tested was built based on the distance data obtained.【Result】 The intergeneric and interspecific sequence variations of matK were the highest among four coding regions tested, and had significant difference from other regions studied. On the contrary, no obvious variations were found in the rpoB and rpoC1 regions. The sequence variation of rbcL was medium among the fragments sequenced. 【Conclusion】 The matK sequence could be used as potential candidate fragment for future DNA barcoding study of Citrus and its closely related genera.

Key words: Citrus, related genera, DNA barcoding, coding sequence

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