枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定

doi:10.3864/j.issn.0578-1752.2010.17.008

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (17): 3547-3554 .doi: 10.3864/j.issn.0578-1752.2010.17.008

• PLANT PROTECTION • Previous Articles Next Articles

Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1

LI Bao-qing, LU Xiu-yun, GUO Qing-gang, QIAN Chang-di, LI She-zeng, MA Ping

(河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心)

Received:2010-02-01 Revised:2010-03-13 Online:2010-09-01 Published:2010-09-01
Contact: MA Ping

Abstract

Abstract:

【Objective】 The objective of this study was to isolate and identify the lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1, and to analyze their antifungal activities. 【Method】 Lipopepetide compounds were isolated, purified and identified by using HPLC and MALDI-TOF-MS techniques, and the volatile compounds (VCs) were identified by using GC-MS method. 【Result】 Three fractions of the lipopeptides, LP-1, LP-2 and LP-3, were collected, among which LP-2 showed the major antifungal activity. According to the mass spectra analysis, LP-1 was determined to be identical to surfactin, varying in the length of the fatty acid chain moiety from C13 to C15. LP-2 consisted of fengycin A (C14—C15) and fengycin B (C14—C18), and LP-3 remained undetermined. Many different kinds of VCs were identified, such as alcohols, ketones, acids, amines, alkanes and so on. 【Conclusion】 B. subtilis strain BAB-1 can produce lipopeptides and VCs both with strong antifungal activities.

Key words: Bacillus subtilis, lipopeptide, volatile compound, inhibition activity

LI Bao-qing,LU Xiu-yun,GUO Qing-gang,QIAN Chang-di,LI She-zeng,MA Ping
. Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1
[J].Scientia Agricultura Sinica, 2010, 43(17): 3547-3554 .

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