Abstract:

【Objective】 A novel OZR gene was cloned from wheat induced by the stripe rust pathogen, and the expression levels were evaluated in wheat challenged by Puccinia striiformis f. sp. tritici or abiotic stress. 【Method】 A cDNA sequence was isolated from wheat cultivar Suwon 11 infected by stripe rust pathogen using in silico cloning and RT-PCR methods. Bioinformatics tools were applied to analyse both the cDNA sequence and protein sequence. The expression patterns of the gene in the interaction of wheat and stripe rust pathogen, exogenous phytohomones and abiotic stresses were investigated using real time quantitative (qRT-PCR). 【Result】 The wheat OZR gene was obtained from wheat infected by stripe rust pathogen, which was designated TaOZR. The opening reading frame (ORF) of TaOZR was 240 bp in length, encoding 79 amino acids with molecular weight 8.67 kD and theoretical pI 9.34. TaOZR containing a signal peptide and a transmembrane might be a secreted protein. TaOZR shared 76% similarity with rice. The transcriptional accumulation of TaOZR was increased in incompatible interaction, and unchanged in compatible interaction. The expression levels of TaOZR were up-regualted by exogenous hormone salicylic acid, ethylene, abascisci acid, jasmonic acid treatments. However, the transcripts of TaOZR had no change in response to various abiotic stresses like low temperature, drought and high salinity. 【Conclusion】 TaOZR gene was firstly cloned and characterized from wheat infected by stripe rust fungus. TaOZR might facilitate wheat defence to stripe rust pathogen through salicylic acid ethylene, abascisci acid, jasmonic acid signal pathways.

Key words: stripe rust')">stripe rust, qRT-PCR, expression profiling, TaOZR, activated oxygen species