Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (03): 639-642 .doi: 10.3864/j.issn.0578-1752.as-2003-2063

• RESEARCH NOTES • Previous Articles    

PCR Amplification, Cloning and Sequence Analysis of the ITS and 5.8S rDNA of Oesophagostomum Isolates

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  1. 华南农业大学兽医学院
  • Received:2003-12-25 Revised:2004-10-19 Online:2005-03-10 Published:2005-03-10

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Abstract: The internal transcribed spacer (ITS) and 5.8S rDNA of Oesophagostomum spp isolated from Guangdong Province was amplified by PCR using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector. The inserts were successfully sequenced, and the results revealed that the inserts were 828 bp in length and consisted of partial 18S, 28S, and complete ITS-1, 5.8S and ITS-2 DNA sequences. The Oesophagostomum isolates were identified as O.dentatum based on ITS-2 sequence. It was the first time that the complete sequence of ITS-1 and 5.8S rDNA of Oesophagostomum dentatum was reported. The results of the present study have laid a foundation for further studies of Oesophagostomum.

Key words: Oesophagostomum, ITS, 5.8S, PCR, Cloning, Sequence analysis

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