Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (14): 3094-3104.doi: 10.3864/j.issn.0578-1752.2026.14.008

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Soil Aggregates in Wheat Fields and Their Carbon and Nitrogen Distribution Characteristics in Response to Green Manure Application Combined with Reduced Nitrogen Fertilizer Application

FENG ZhenKang(), YU AiZhong(), YIN Bo, ZHANG DongLing, CAI HongWei, HOU ShiHe, HUANG YaLi, YU YanZi, HUO JianZhe, PANG XiaoNeng   

  1. College of Agronomy, Gansu Agricultural University/State Key Laboratory of Arid Land Crop Science, Lanzhou 730070
  • Received:2025-09-16 Accepted:2025-11-13 Online:2026-07-16 Published:2026-07-21
  • Contact: YU AiZhong

Abstract:

【Objective】Based on a long‑term field experiment, this study investigated the response of soil aggregate distribution characteristics, along with their associated organic carbon and total nitrogen contents, to green manure incorporation combined with nitrogen reduction. The aim was to provide a theoretical basis for developing rational soil fertility management practices in wheat production in this region.【Method】Based on seven treatments from a long‑term field experiment initiated in 2018: N100 (no green manure incorporation with 180 kg∙hm-2 nitrogen), G1N85 (15 000 kg∙hm-2 green manure+153 kg∙hm-2 nitrogen), G2N85 (22 500 kg∙hm-2 green manure+153 kg∙hm-2 nitrogen), G3N85 (30 000 kg∙hm-2 green manure+153 kg∙hm-2 nitrogen), G1N70 (15 000 kg∙hm-2 green manure+127 kg∙hm-2 nitrogen), G2N70 (22 500 kg∙hm-2 green manure+127 kg∙hm-2 nitrogen), and G3N70 (30 000 kg∙hm-2 green manure+127 kg∙hm-2 nitrogen). Using data from this field experiment collected in 2024 and 2025, we analyzed the effects of these treatments on soil aggregate composition and carbon‑nitrogen nutrient contents.【Result】Compared to N100, the combination of green manure incorporation and reduced nitrogen fertilizer application not only significantly increased the content of large soil aggregates (>0.25 mm) in the 0-20 cm and 20-40 cm soil layers of wheat fields but also enhanced the mean weight diameter (MWD) and geometric mean diameter (GMD), with the G3N85 treatment yielding the optimal effect. Compared to other treatments, G3N85 increased the organic carbon and total nitrogen content of all particle size agglomerates. with increases in organic carbon content of 0.7%-12.9%, 1.3%-12.4%, 1.2%-15.0%, and 3.2%-13.4% for aggregates >2 mm, 2-0.25 mm, 0.25-0.053 mm, and <0.053 mm, respectively. while aggregate total nitrogen increased by 3.1%-11.4%, 3.6%-11.1%, 2.1%-13.1%, and 4.9%-10.9%, respectively. Moreover, the contribution rates of organic carbon and total nitrogen in large aggregates to soil organic carbon and total nitrogen significantly increased under this treatment, with increases of 19.7%, 23.3%, 19.6%, and 24.2%, respectively. Conversely, the contribution rates of organic carbon and total nitrogen from micro-aggregates and silt-clay particles to soil organic carbon and total nitrogen significantly decreased.【Conclusion】The combination of green manure incorporation and reduced nitrogen fertilizer application not only increased the content and stability of large soil aggregates in the 0-40 cm layer but also enhanced the contribution rates of organic carbon and total nitrogen in these aggregates. The optimal treatment was applying 30 000 kg∙hm-2 of green manure combined with 153 kg∙hm-2 of nitrogen fertilizer. This regimen can serve as a rational fertilization system for maintaining soil structure stability and promoting carbon-nitrogen accumulation in wheat fields in the Hexi Oasis irrigation district.

Key words: wheat, green manure incorporation, reduced nitrogen fertilizer application, aggregate composition, aggregate stability, carbon-nitrogen distribution in aggregates

Fig. 1

Daily precipitation and mean air temperature during the whole growth period of wheat in the experimental station in 2024, 2025"

Table 1

Green manure returning combined with nitrogen fertilizer reduction experimental program"

处理代码
Treatment code
肥料施用量Fertilizer application rate (kg∙hm-2)
绿肥
Green manure
磷肥(P2O5)
Phosphate fertilizer
氮肥(N)
Nitrogen fertilizer
N100 0 90 180
G1N85 15 000 90 153
G2N85 22 500 90 153
G3N85 30 000 90 153
G1N70 15 000 90 126
G2N70 22 500 90 126
G3N70 30 000 90 126

Fig. 2

Proportion of soil aggregates with different particle sizes under different treatments"

Fig. 3

Mean soil aggregate weight diameter and geometric mean diameter under different treatments"

Fig. 4

Organic carbon and total nitrogen content in 0-40 cm soil layer under different treatments"

Fig. 5

The organic carbon and total nitrogen content in different particle sizes aggregates under different treatments"

Table 2

Contributions of OC and TN in different size aggregates of each treatment to soil OC and TN"

年份
Years
土层
Soil layer (cm)
处理
Treatment
有机碳贡献率 OC contribution rate (%) 全氮贡献率 TN contribution rate (%)
2 mm 2-0.25 mm 0.25-0.053 mm <0.053 mm 2-0.25 mm 0.25-0.053 mm <0.053 mm
2024 0-20 N100 5.1b 41.7b 25.6ab 19.2a 5.2c 42.8b 21.3a 21.1a
G1N85 6.4b 45.1ab 22.4abc 19.1a 6.1abc 43.5ab 22.0a 19.9b
G2N85 7.0a 45.4ab 22.3bc 18.3a 6.8ab 44.8ab 20.9a 19.6b
G3N85 7.7a 48.3a 20.0c 18.0a 7.5a 48.5a 17.9a 19.4b
G1N70 6.1ab 43.8b 26.3a 19.3a 5.9bc 44.4ab 21.0a 20.1b
G2N70 6.7a 44.9ab 21.3bc 18.7a 6.5bc 44.3ab 21.6a 19.7b
G3N70 7.2a 46.0ab 21.8c 18.0a 7.1b 45.5ab 20.0a 19.4b
20-40 N100 4.4d 39.2c 28.2a 19.0a 4.2d 38.7c 26.6ab 20.4a
G1N85 5.3c 41.8bc 25.7ab 18.7a 5.1d 41.2c 24.5b 20.0a
G2N85 5.8bc 43.5c 24.1abc 18.9a 5.5bc 42.6bc 22.6b 20.0a
G3N85 6.8a 47.7a 20.3c 18.5a 6.4a 46.9a 19.1c 19.6ab
G1N70 5.5c 42.2bc 25.6b 18.8a 4.8cd 38.8c 29.1a 19.6ab
G2N70 5.7c 43.4c 23.9abc 18.8a 5.1bcd 40.5d 27.0ab 19.5ab
G3N70 6.5ab 44.8b 23.0bc 18.6a 6.1b 44.2ab 23.9b 19.0c
2025 0-20 N100 5.0c 41.2c 25.7a 19.8a 5.2d 39.9e 22.3a 21.3ab
G1N85 6.5b 44.5c 22.6bc 18.7a 6.6c 44.6c 22.9a 20.1ab
G2N85 6.9ab 45.2bc 21.4bcd 18.7a 6.9b 43.9bcd 20.8ab 19.7ab
G3N85 7.9a 49.1a 19.4d 18.0a 7.8a 47.8a 18.4b 18.8b
G1N70 6.2bc 43.9bc 23.2b 18.9a 5.7cd 41.5d 20.9ab 18.2b
G2N70 6.7ab 44.6bc 21.4bcd 18.6a 6.5c 43.0de 21.0ab 18.9b
G3N70 7.3ab 46.4b 20.7cd 17.9a 7.3ab 45.9ab 20.2ab 19.0b
20-40 N100 4.6c 39.6c 28.3a 18.9a 4.3c 39.2e 27.3a 20.2e
G1N85 5.4b 43.7b 26.0abc 18.1a 5.0c 42.7bc 26.3ab 19.2bc
G2N85 6.0b 44.9ab 24.7bc 19.6a 5.5b 43.1bc 24.8ab 20.2bc
G3N85 6.8a 47.2a 20.9d 17.4a 6.5a 47.3bc 20.9c 18.7a
G1N70 5.9b 42.2c 26.5b 18.5a 5.3b 40.1e 26.5ab 19.3de
G2N70 5.7b 41.9c 23.9bc 18.2a 5.3b 41.5cd 24.4ab 19.7cd
G3N70 6.8a 44.7ab 23.4cd 17.6a 6.4a 44.6b 23.4bc 18.4b
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