Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (14): 3006-3021.doi: 10.3864/j.issn.0578-1752.2026.14.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Evaluation of Rust Resistance in the Seedling Stage of Wheat Varieties and Disease-Resistant Gene Detection in Shanxi Province, China

MAO JiaQi1,2(), WU BangBang1, ZHAO JiaJia1, HAO YuQiong1, ZHENG XingWei1, WU JianHui4, ZENG QingDong4, LIU MinJie3(), ZHENG Jun1()   

  1. 1 Institute of Wheat Research, Shanxi Agriculture University/Key Laboratory of Sustainable Dryland Agriculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Linfen 041000, Shanxi
    2 College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi
    3 College of Plant Protection, Shanxi Agricultural University/Key Laboratory of Agricultural Integrated Pest Management in Shanxi, Taiyuan 030031
    4 College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi
  • Received:2025-12-12 Accepted:2026-03-18 Online:2026-07-16 Published:2026-07-21
  • Contact: LIU MinJie, ZHENG Jun

RichHTML

5

PDF

13

Abstract:

【Objective】Stripe rust and leaf rust are major diseases affecting wheat production in the Huang-Huai winter wheat region, accurate identification of resistance in wheat cultivars, understanding the distribution of disease-resistance genes, excavate new disease-resistance genes/loci lays the foundation for the breeding and promotion of disease-resistant varieties.【Method】We phenotyped 326 Shanxi wheat at both seedling against the prevalent Chinese Pst races CYR32 and CYR34, and Pt race PHSH. To identify more Yr/Lr genes, we also conducted molecular detection and GBTS, using a genome-wide association study (GWAS) using a wheat 16K SNP array.【Result】Shanxi wheat seedlings demonstrate generally good resistance to stripe rust during the seedling stage, 97 cultivars such as Jinmai 919, Yunmai 766 and Linhei 199 showed good resistance to the Pst isolate CYR32 and CYR34 during the seedling stage; 15 (4.60%) cultivars such as Zhonghan 110, Zeyou 2 and Shinong 086 showed resistance to the isolate Pst isolate CYR32;14 (4.29%) cultivars such as Jinnong 207, Jinmai 13 and Linhei 199 showed resistance to the isolate Pst isolate CYR34; wheat seedlings in Shanxi Province exhibit poor resistance to leaf rust during the seedling stage, 12 cultivars showed moderate resistance to the isolate Pt isolate PHSH. Of the 23 Yr/Lr genes assayed, a total of eleven stripe rust resistance genes/QTLs have been detected, including QYrqin.nwafu-2AL (87.42%), Yr78 (27.30%), Yr9 (24.54%), QYrsn.nwafu-3DL (15.95%), Yr75 (13.19%), QYrhm.nwafu-2BC (10.73%), Yr5 (7.98%), Yr80 (2.76%), QYr.nwafu-4BL (2.15%), YrSP (1.84%) and Yr82 (1.84%), as well as seven leaf rust resistance genes, including Lr46 (28.83%), Lr1 (22.09%), Lr68 (16.87%), Lr26 (16.56%), Lr34 (11.96%), Lr13 (5.83%) and Lr37 (1.23%) were identified in wheat varieties from Shanxi province, whereas Yr15, Yr26, Lr10, Lr21 and Lr80 were not detected; for the pyramiding of multiple Yr/Lr genes, the highest proportion was the QYrqin.nwafu-2AL+QYrsn.nwafu-3DL and Lr46+Lr68 combinations. GWAS identified five loci significantly associated with stripe rust resistance, 2B, 5B and 7A may harbor novel loci associated with stripe rust resistance, simultaneously identified fourteen loci significantly associated with resistance to leaf rust disease, 1B, 4A and 5A may harbor novel loci associated with stripe rust resistance.【Conclusion】Shanxi wheat exhibits generally good resistance to stripe rust during the seedling stage, but overall resistance to leaf rust is poor, thirty-eight loci significantly associated with stripe rust/leaf rust resistance were mapped across 13 chromosomes, including 1B, 2B, and 3B. Preliminary analysis suggests that fourteen of these loci, including QYrs-2B, QLrs-2A and QLrs-4A may represent novel rust resistance loci.

Key words: Shanxi wheat, stripe rust, leaf rust, resistance gene, GWAS

Table 1

Molecular markers for wheat rust resistance genes detection"

基因 Gene 标记 Marker 引物序列 Primer sequence (5′-3′) 参考文献 References
Lr1 WR003F GGGACAGAGACCTTGGTGGA [35]
WR003R GACGATGATGATTTGCTGCTGG
Lr10 Lrk10D1 GAAGCCCTTCGTCTCATCTG [36]
Lrk10D2 TTGATTCATTGCAGATGAGATCACG
Lr21 D14-L CGCTTTTACCGAGATTGGTC [37]
D14-R TCTGGTATCTCACGAAGCCTT
Lr26+ ω-secalinF ACCTTCCTCATCTTTGTCCT [38]
ω-secalinR CCGATGCCTATACCACTACT
Lr26- Glu-B3F GGTACCAACAACAACAACCC
Glu-B3R GTTGCTGCTGAGGTTGGTTC
Lr34 csLV34F GTTGGTTAAGACTGGTGATGG [39]
csLV34R TGCTTGCTATTGCTGAATAGT
Yr5 S19M93-100-F TAATTGGGACCGAGAGACG [40]
S19M93-100-R TTCTTGCAGCTCCAAAACCT
Yr9 H20-F GTTGGAAGGGAGCTCGAGCTG [41]
H20-R GTTGGGCAGAAAGGTCGACATC
Yr15 uhw301R-F GGAGATAGAGCACATTACAGAC [12]
uhw301R-R TTTCGCATCCCACCCTACTG

Fig. 1

Standards for phenotypic identification and disease resistance level statistics of wheat stripe rust/leaf rust in Shanxi A: Disease incidence on seedling leaves of some Shanxi wheat varieties inoculated with CYR34; B: Disease incidence on seedling leaves of some Shanxi wheat varieties inoculated with PHSH; C: Seedling infection types of stripe rust in Shanxi wheat; D: Seedling infection types of leaf rust in Shanxi wheat; E: Statistics of stripe rust/leaf rust resistance levels in Shanxi wheat. HS: Highly susceptibility; MS: Middle susceptibility; MR: Middle resistant; HR: Highly resistant; IM: Immune"

Fig. 2

Frequency distribution of stripe rust/leaf rust resistance genes in Shanxi wheat varieties A: Distribution of stripe rust genes; B: Distribution of major stripe rust resistance gene combinations; C: Distribution frequency of stripe rust resistance genes in varieties of different breeding years"

Fig. 3

Partial PCR detection marker electrophoresis diagram of some stripe rust/leaf rust resistance genes A: Lr1 molecular marker WR003, 1: Shunmai 1718, 2: NC206, 3: Jinmai 102, 4: Linfen 6410, 5: Yunmai 2064, 6: Shengmai 104, 7: Jinmai 17, 8: Jinmai 38; B: Lr26 molecular marker ω-secalin, 1: Chang 6990, 2: Zhongmai 247, 3: Linhan 6, 4: Lin Y8161, 5: Zhonghan 110, 6: Lühan 1608; C: Lr34 with the csLV34 marker, 1: Baihuomai, 2: Jiahongmai, 3: Qisifeng, 4: Baikehong, 5: Siyuehuang, 6: Sanyuehuang, 7: Hongxiaomai, 8: Jinmai 70, 9: Linfen 139; D: Lr26 molecular marker Glu-B3, 1: Chang 6990, 2: Zhongmai 247, 3: Linhan 6, 4: Lin Y8161, 5: Zhonghan 110, 6: Lühan 1608; E: Yr5 molecular marker S19M93-100, 1: Jinmai 33, 2: Jinmai 63, 3: Linmai 5311, 4: Linhei 199, 5: Linhan 6, 6: Jinmai 37, 7: Jinchun 3, 8: Shinnong 086; F: Yr9 molecular marker H20, 1: Yunhan 1818, 2: Linhan 5325, 3: Zhongmai 110, 4: Linfen 139, 5: Changmai 3987, 6: Shunmai 612, 7: Zeyou 1. M denotes marker DL 50 (C, E) and DL 5000 (A-B, D and F); +: contains disease resistant genes; -: indicates the absence of disease resistant genes"

Fig. 4

Frequency distribution of leaf rust resistance genes in Shanxi wheat varieties A: Distribution of leaf rust genes; B: Distribution of major leaf rust resistance gene combinations; C: Distribution frequency of leaf rust resistance genes in varieties of different breeding years"

Table 2

SNPs significantly associated with wheat stripe rust resistance"

生理小种
Physiological race		 标记
Marker		 染色体
Chromosome		 物理位置
Physical position (Mb)		 P值
P value		 表型贡献率
R2 (%)		 参考文献
References
CYR32 QYrs-2B 2B 394.98 3.16 5.11
QYrs-7A 7A 450.89 3.01 4.90
CYR34 QYrs-3B 3B 69.81 3.07 5.03 [43]
QYrs-3D 3D 344.36 3.09 5.07 [44]
QYrs-5B 5B 40.90 4.03 5.97
PHSH QLrs-1B 1B 276.31—310.58 3.00—3.64 5.58
QLrs-1B.1 1B 388.87 3.50 5.74—6.24
QLrs-1B.2 1B 546.67—615.58 3.21—3.38 4.90—6.02 [45]
QLrs-2A 2A 709.15—712.70 3.15—3.71 4.82—5.69
QLrs-2B 2B 593.63 3.14 4.79 [46]
QLrs-2B.1 2B 710.72 3.77 6.69
QLrs-4A 4A 108.38—132.33 3.84 6.81—7.62
QLrs-4B.1 4B 295.96—329.77 3.63—3.82 5.87—5.90
QLrs-5A 5A 314.16—355.87 3.05—3.09 4.66—4.72
QLrs-5A.1 5A 364.19—377.11 3.07—3.84 4.68—5.90
QLrs-6B 6B 633.89—704.89 3.03—3.39 4.62—6.42 [47]
QLrs-6D 6D 151.83—159.39 4.13—3.46 5.26—6.35
QLrs-6D.1 6D 237.98—258.61 3.35—3.89 5.13—5.97
QLrs-7B 7B 477.99—533.57 3.15—3.33 4.82—5.95

Fig. 5

Genome-wide association study Manhattan plot and haplotype analysis A: Manhattan plot of genome-wide association analysis for stripe rust/leaf rust phenotypes at the seedling stage; B-F: Relationship between chromosome haplotypes and stripe rust phenotype; G-J: Relationship between chromosome haplotypes and leaf rust phenotype; * and *** indicate significance at P<0.05, and P<0.001, respectively; ns indicates not significant"

[1]
康振生, 王晓杰, 赵杰, 汤春蕾, 黄丽丽. 小麦条锈菌致病性及其变异研究进展[J]. 中国农业科学, 2015, 48(17): 3439-3453. DOI: 10.3864/j.issn.0578-1752.2015.17.011.
Kang Z S, Wang X J, Zhao J, Tang C L, Huang L L. Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f. sp. tritici[J]. Scientia Agricultura Sinica, 2015, 48(17): 3439-3453. DOI: 10.3864/j.issn.0578-1752.2015.17.011. (in Chinese)
[2]
Bolton M D, Kolmer J A, Garvin D F. Wheat leaf rust caused by Puccinia triticina[J]. Molecular Plant Pathology, 2008, 9(5): 563-575.

doi: 10.1111/mpp.2008.9.issue-5
[3]
Chen X M, Kang Z S. Stripe Rust[M]. Dordrecht: Springer Netherlands, 2017.
[4]
刘万才, 王保通, 赵中华, 李跃, 康振生. 我国小麦条锈病历次大流行的历史回顾与对策建议[J]. 中国植保导刊, 2022, 42(6): 21-27, 41.
Liu W C, Wang B T, Zhao Z H, Li Y, Kang Z S. Historical review and countermeasures of wheat stripe rust epidemics in China[J]. China Plant Protection, 2022, 42(6): 21-27, 41. (in Chinese)
[5]
Gebrewahid T W, Yao Z J, Yan X C, Gao P, Li Z F. Identification of leaf rust resistance genes in Chinese common wheat cultivars[J]. Plant Disease, 2017, 101(10): 1729-1737.

doi: 10.1094/PDIS-02-17-0247-RE pmid: 30676921
[6]
孙海燕, 原征, 疏燕, 张兴, 张爱香, 陈怀谷. 不同杀菌剂对小麦赤霉病和叶锈病的防治效果[J]. 植物保护, 2021, 47(1): 273-276.
Sun H Y, Yuan Z, Shu Y, Zhang X, Zhang A X, Chen H G. Control efficacy of different fungicides against Fusarium head blight and leaf rust in wheat[J]. Plant Protection, 2021, 47(1): 273-276. (in Chinese)
[7]
Rimbaud L, Papaïx J, Rey J F, Barrett L G, Thrall P H. Assessing the durability and efficiency of landscape-based strategies to deploy plant resistance to pathogens[J]. PLoS Computational Biology, 2018, 14(4): e1006067.

doi: 10.1371/journal.pcbi.1006067
[8]
Sharma D, Avni R, Gutierrez-Gonzalez J, Kumar R, Sela H, Prusty M R, Shatil-Cohen A, Molnár I, Holušová K, Said M, Doležel J, Millet E, Khazan-Kost S, Landau U, Bethke G, Sharon O, Ezrati S, Ronen M, Maatuk O, Eilam T, et al. A single NLR gene confers resistance to leaf and stripe rust in wheat[J]. Nature Communications, 2024, 15: 9925.

doi: 10.1038/s41467-024-54068-6
[9]
李峰奇, 韩德俊, 魏国荣, 曾庆东, 黄丽丽, 康振生. 黄淮麦区126个小麦品种(系)抗条锈病基因的分子检测[J]. 中国农业科学, 2008, 41(10): 3060-3069. DOI: 10.3864/j.issn.0578-1752.2008.10.019.
Li F Q, Han D J, Wei G R, Zeng Q D, Huang L L, Kang Z S. Molecular detection of stripe rust resistant genes in 126 winter wheat varieties from the Huanghuai wheat region[J]. Scientia Agricultura Sinica, 2008, 41(10): 3060-3069. DOI: 10.3864/j.issn.0578-1752.2008.10.019. (in Chinese)
[10]
郑慧敏, 温晓蕾, 郝晨阳, 张培培, GEBREWAHID Takele Weldu, 闫晓翠, 刘大群, 张学勇, 李在峰. 70份国外小麦品种(系)的苗期和成株期抗叶锈病鉴定[J]. 作物学报, 2019, 45(10): 1455-1467.

doi: 10.3724/SP.J.1006.2019.91003
Zheng H M, Wen X L, Hao C Y, Zhang P P, Weldu G, Yan X C, Liu D Q, Zhang X Y, Li Z F. Seedling and slow rusting resistance to leaf rust in 70 introduced wheat lines[J]. Acta Agronomica Sinica, 2019, 45(10): 1455-1467. (in Chinese)

doi: 10.3724/SP.J.1006.2019.91003
[11]
Marchal C, Zhang J P, Zhang P, Fenwick P, Steuernagel B, Adamski N M, Boyd L, McIntosh R, Wulff B B H, Berry S, Lagudah E, Uauy C. BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust[J]. Nature Plants, 2018, 4(9): 662-668.

doi: 10.1038/s41477-018-0236-4 pmid: 30150615
[12]
Klymiuk V, Yaniv E, Huang L, Raats D, Fatiukha A, Chen S S, Feng L H, Frenkel Z, Krugman T, Lidzbarsky G, Chang W, Jääskeläinen M J, Schudoma C, Paulin L, Laine P, Bariana H, Sela H, Saleem K, Sørensen C K, Hovmøller M S, et al. Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family[J]. Nature Communications, 2018, 9: 3735.

doi: 10.1038/s41467-018-06138-9
[13]
William M, Singh R P, Huerta-Espino J, Islas S O, Hoisington D. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat[J]. Phytopathology, 2003, 93(2): 153-159.

doi: 10.1094/PHYTO.2003.93.2.153 pmid: 18943129
[14]
Ellis J G, Lagudah E S, Spielmeyer W, Dodds P N. The past, present and future of breeding rust resistant wheat[J]. Frontiers in Plant Science, 2014, 5: 641.

doi: 10.3389/fpls.2014.00641 pmid: 25505474
[15]
Wu J H, Ma S W, Niu J Q, Sun W H, Dong H T, Zheng S S, Zhao J W, Liu S J, Yu R, Li Y, Han J Y, Wang Y M, Chen T T, Zhang C L, Zhang W J, Ding B Y, Chang L, Xue W B, Zheng W J, Li C L, et al. Genomics-driven discovery of superior alleles and genes for yellow rust resistance in wheat[J]. Nature Genetics, 2025, 57(8): 2017-2027.

doi: 10.1038/s41588-025-02259-2
[16]
Zhang Y C, Chen G, Zang Y M, Bhavani S, Bai B, Liu W, Zhao M M, Cheng Y K, Li S D, Chen W, Yan W H, Mao H L, Su H D, Singh R P, Lagudah E, Li Q, Lan C X. Lr34/Yr18/Sr57/Pm38 confers broad-spectrum resistance to fungal diseases via sinapyl alcohol transport for cell wall lignification in wheat[J]. Plant Communications, 2024, 5(12): 101077.

doi: 10.1016/j.xplc.2024.101077
[17]
贾秋珍, 曹世勤, 王晓明, 孙振宇, 张勃, 黄瑾, 骆惠生, 李青青. 2019年—2020年甘肃省小麦条锈菌生理小种变异监测[J]. 植物保护, 2022, 48(5): 327-332.
Jia Q Z, Cao S Q, Wang X M, Sun Z Y, Zhang B, Huang J, Luo H S, Li Q Q. Monitoring the variation of physiological races of Puccinia striiformis f.sp. tritici in Gansu Province during 2019-2020[J]. Plant Protection, 2022, 48(5): 327-332. (in Chinese)
[18]
李明菊, 陈贤明, 万安民, 丁明亮, 程加省. 云南小麦条锈菌群体对18个抗条锈近等基因系的毒性分析[J]. 植物保护学报, 2018, 45(1): 75-82.
Li M J, Chen X M, Wan A M, Ding M L, Cheng J S. Virulence characterization of stripe rust pathogen Puccinia striiformis f. sp. tritici population to 18 near-isogenic lines resistant to wheat yellow rust in Yunnan Province[J]. Journal of Plant Protection, 2018, 45(1): 75-82. (in Chinese)
[19]
武延超, 孟庆芳, 杨文香, 温晓蕾, 刘大群. 2007年陕西、湖北和四川三省小麦叶锈菌苗期毒性分析[J]. 中国农学通报, 2009, 25(17): 195-198.
Wu Y C, Meng Q F, Yang W X, Wen X L, Liu D Q. Virulence of Puccinia triticina on wheat at seedling stage in Shaanxi, HuBei and Sichuan Provinces of China in 2007[J]. Chinese Agricultural Science Bulletin, 2009, 25(17): 195-198. (in Chinese)
[20]
葛润静, 刘太国, 高利, 刘博, 陈万权. 2011—2012年度中国六省小麦叶锈菌群体毒性分析[J]. 植物病理学报, 2015, 45(2): 175-180.

doi: 10.13926/j.cnki.apps.2015.02.008
Ge R J, Liu T G, Gao L, Liu B, Chen W Q. Virulence of Puccinia triticina from 6 provinces in China in 2011-2012[J]. Acta Phytopathologica Sinica, 2015, 45(2): 175-180. (in Chinese)
[21]
习玲, 王昱琦, 朱微, 王益, 陈国跃, 蒲宗君, 周永红, 康厚扬. 78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测[J]. 作物学报, 2021, 47(7): 1309-1323.

doi: 10.3724/SP.J.1006.2021.01061
Xi L, Wang Y Q, Zhu W, Wang Y, Chen G Y, Pu Z J, Zhou Y H, Kang H Y. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan Province[J]. Acta Agronomica Sinica, 2021, 47(7): 1309-1323. (in Chinese)

doi: 10.3724/SP.J.1006.2021.01061
[22]
郑良梅, 杨慧, 徐晓伟, 童朝阳, 蔺瑞明, 冯晶, 姚强, 王凤涛. 107个山东小麦品种抗条锈性评价及分子标记检测[J]. 麦类作物学报, 2023, 43(8): 968-976.
Zheng L M, Yang H, Xu X W, Tong Z Y, Lin R M, Feng J, Yao Q, Wang F T. Resistance evaluation and molecular detection of resistant genes to stripe rust of 107 wheat varieties in Shandong Province[J]. Journal of Triticeae Crops, 2023, 43(8): 968-976. (in Chinese)
[23]
王炜星, 张梦宇, 董瑞, 张培培, 张佳瑶, 李在峰, 刘大群. 河南71个重要小麦生产品种的抗叶锈性鉴定[J]. 麦类作物学报, 2022, 42(3): 279-288.
Wang W X, Zhang M Y, Dong R, Zhang P P, Zhang J Y, Li Z F, Liu D Q. Identification of leaf rust resistance of 71 wheat cultivars from Henan Province[J]. Journal of Triticeae Crops, 2022, 42(3): 279-288. (in Chinese)
[24]
徐云碧, 杨泉女, 郑洪建, 许彦芬, 桑志勤, 郭子锋, 彭海, 张丛, 蓝昊发, 王蕴波, 吴坤生, 陶家军, 张嘉楠. 靶向测序基因型检测(GBTS)技术及其应用[J]. 中国农业科学, 2020, 53(15): 2983-3004. DOI: 10.3864/j.issn.0578-1752.2020.15.001.
Xu Y B, Yang Q N, Zheng H J, Xu Y F, Sang Z Q, Guo Z F, Peng H, Zhang C, Lan H F, Wang Y B, Wu K S, Tao J J, Zhang J N. Genotyping by target sequencing (GBTS) and its applications[J]. Scientia Agricultura Sinica, 2020, 53(15): 2983-3004. DOI: 10.3864/j.issn.0578-1752.2020.15.001. (in Chinese)
[25]
Liu L, Han J Y, Zhang D D, Li Y Q, Zhang C L, Liu S J, Yan Z Y, Zheng W J, Li C L, Zeng Q D, Kang Z S, Han D J, Zhang W T, Yao Q, Wu J H. Genotyping by target sequencing (GBTS)-based genetic mapping uncovers synergistic interactions for stripe rust resistance in wheat cultivar Flanders[J]. Theoretical and Applied Genetics, 2025, 138(8): 187.

doi: 10.1007/s00122-025-04969-x pmid: 40689963
[26]
陈建雄, 严浩浩, 曹世勤, 贾秋珍, 李彦兵, 伏松平, 韩德俊, 康振生, 曾庆东, 姚强. 62份甘肃小麦品种(系)抗条锈性评价及抗病基因检测[J]. 植物病理学报, 2023, 53(5): 881-889.

doi: 10.13926/j.cnki.apps.001015
Chen J X, Yan H H, Cao S Q, Jia Q Z, Li Y B, Fu S P, Han D J, Kang Z S, Zeng Q D, Yao Q. Evaluation of resistance and detection of resistance gene(s)to stripe rust of 62 wheat cultivars(lines)in Gansu Province[J]. Acta Phytopathologica Sinica, 2023, 53(5): 881-889. (in Chinese)
[27]
徐兆飞. 山西小麦[M]. 北京: 中国农业出版社, 2006.
Xu Z F. Shanxi Wheat[M]. Beijing: China Agriculture Press, 2006. (in Chinese)
[28]
庞云星, 崔宏梅, 蔺瑞明, 杨慧, 冯晶, 马东方, 徐世昌, 王凤涛. 89份河北、山西小麦品种抗条锈性评价及抗条锈病基因检测[J]. 植物保护, 2021, 47(6): 49-57, 92.
Pang Y X, Cui H M, Lin R M, Yang H, Feng J, Ma D F, Xu S C, Wang F T. Resistance evaluation and detection of the resistance genes in 89 wheat cultivars from Hebei and Shanxi Provinces to stripe rust[J]. Plant Protection, 2021, 47(6): 49-57, 92. (in Chinese)
[29]
刘敏捷, 原宗英, 武英鹏. 2015—2018年度山西省小麦区域试验品种及育种材料抗病性评价[J]. 山西农业科学, 2020, 48(4): 489-491.
Liu M J, Yuan Z Y, Wu Y P. Resistance evaluation of wheat regional trail varieties and breeding materials in Shanxi Province in 2015-2018[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(4): 489-491. (in Chinese)
[30]
韩德俊, 王琪琳, 张立, 魏国荣, 曾庆东, 赵杰, 王晓杰, 黄丽丽, 康振生. “西北-华北-长江中下游”条锈病流行区系当前小麦品种(系)抗条锈病性评价[J]. 中国农业科学, 2010, 43(14): 2889-2896. DOI: 10.3864/j.issn.0578-1752.2010.14.007.
Han D J, Wang Q L, Zhang L, Wei G R, Zeng Q D, Zhao J, Wang X J, Huang L L, Kang Z S. Evaluation of resistance of current wheat cultivars to stripe rust in northwest China, North China and the middle and lower reaches of Changjiang River epidemic area[J]. Scientia Agricultura Sinica, 2010, 43(14): 2889-2896. DOI: 10.3864/j.issn.0578-1752.2010.14.007. (in Chinese)
[31]
王佳真, 李在峰, 李星, 刘大群. 小麦品系5R618抗叶锈病基因的初步定位[J]. 植物遗传资源学报, 2014, 15(6): 1348-1351.

doi: 10.13430/j.cnki.jpgr.2014.06.025
Wang J Z, Li Z F, Li X, Liu D Q. Genetic analysis of leaf rust resistance gene in wheat line 5R618[J]. Journal of Plant Genetic Resources, 2014, 15(6): 1348-1351. (in Chinese)
[32]
Line R F, Qayoum A. Virulence aggressiveness, evolution and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968—87: 1788[R]. US Department of Agriculture, Agricultural Research Service, 1992.
[33]
Roelfs A P, Singh R P, Saari E E. Rust Diseases of Wheat: Concepts and Methods of Disease Management[M]. CIMMYT, Mexico, D.F., 1992.
[34]
Guo Z F, Wang H W, Tao J J, Ren Y H, Xu C, Wu K S, Zou C, Zhang J N, Xu Y B. Development of multiple SNP marker panels affordable to breeders through genotyping by target sequencing (GBTS) in maize[J]. Molecular Breeding, 2019, 39(3): 37.

doi: 10.1007/s11032-019-0940-4
[35]
Qiu J W, Schürch A C, Yahiaoui N, Dong L L, Fan H J, Zhang Z J, Keller B, Ling H Q. Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat[J]. Theoretical and Applied Genetics, 2007, 115(2): 159-168.

doi: 10.1007/s00122-007-0551-z
[36]
Schachermayr G, Feuillet C, Keller B. Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds[J]. Molecular Breeding, 1997, 3(1): 65-74.

doi: 10.1023/A:1009619905909
[37]
Huang L, Gill B S. An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat[J]. Theoretical and Applied Genetics, 2001, 103(6): 1007-1013.

doi: 10.1007/s001220100701
[38]
Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL·1RS wheat-rye chromosome translocations[J]. Plant Breeding, 2006, 125(3): 302-304.

doi: 10.1111/pbr.2006.125.issue-3
[39]
Lagudah E S, McFadden H, Singh R P, Huerta-Espino J, Bariana H S, Spielmeyer W. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat[J]. Theoretical and Applied Genetics, 2006, 114(1): 21-30.

doi: 10.1007/s00122-006-0406-z pmid: 17008991
[40]
Smith P H, Hadfield J, Hart N J, Koebner R M, Boyd L A. STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster[J]. Genome, 2007, 50(3): 259-265.

doi: 10.1139/g07-004 pmid: 17502899
[41]
Liu C, Yang Z J, Li G R, Zeng Z X, Zhang Y, Zhou J P, Liu Z H, Ren Z L. Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background[J]. Euphytica, 2008, 159(1): 249-258.

doi: 10.1007/s10681-007-9484-5
[42]
卫乃翠, 陶金博, 苑名杨, 张彧, 开梦想, 乔玲, 武棒棒, 郝宇琼, 郑兴卫, 王娟玲, 赵佳佳, 郑军. 山西小麦苗期耐低磷特性及遗传分析[J]. 中国农业科学, 2024, 57(5): 831-845. DOI: 10.3864/j.issn.0578-1752.2024.05.001.
Wei N C, Tao J B, Yuan M Y, Zhang Y, Kai M X, Qiao L, Wu B B, Hao Y Q, Zheng X W, Wang J L, Zhao J J, Zheng J. Seedling characterization and genetic analysis of low phosphorus tolerance in Shanxi varieties[J]. Scientia Agricultura Sinica, 2024, 57(5): 831-845. DOI: 10.3864/j.issn.0578-1752.2024.05.001. (in Chinese)
[43]
Hao Y F, Chen Z B, Wang Y Y, Bland D, Buck J, Brown-Guedira G, Johnson J. Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat[J]. Theoretical and Applied Genetics, 2011, 123(8): 1401-1411.

doi: 10.1007/s00122-011-1675-8 pmid: 21830107
[44]
Bai B, Li Z M, Wang H M, Du X L, Wu L, Du J Y, Lan C X. Genetic analysis of adult plant resistance to stripe rust in common wheat cultivar “pascal”[J]. Frontiers in Plant Science, 2022, 13: 918437.

doi: 10.3389/fpls.2022.918437
[45]
Zhang P P, Lan C X, Asad M A, Gebrewahid T W, Xia X C, He Z H, Li Z F, Liu D Q. QTL mapping of adult-plant resistance to leaf rust in the Chinese landraces Pingyuan 50/Mingxian 169 using the wheat 55K SNP array[J]. Molecular Breeding, 2019, 39(7): 98.

doi: 10.1007/s11032-019-1004-5
[46]
Zhang Y B, Wang Z, Quan W, Zhang X, Feng J, Ren J D, Jiang X, Zhang Z J. Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat Landrace Hongmazha[J]. Theoretical and Applied Genetics, 2021, 134(5): 1363-1376.

doi: 10.1007/s00122-021-03776-4 pmid: 33550471
[47]
Kolmer J A. Leaf rust resistance in wheat line RL6062 is an allele at the Lr3 locus[J]. Crop Science, 2015, 55(5): 2186-2190.

doi: 10.2135/cropsci2015.01.0031
[48]
杨迪, 李岩佳, 蔡奇昌, 黄洁, 李莹, 王保通, 龙书生, 李强. 194份小麦品种(系)抗条锈性和白粉性评价及抗病基因检测[J]. 植物病理学报, 2025, 55(2): 261-269.

doi: 10.13926/j.cnki.apps.000937
Yang D, Li Y J, Cai Q C, Huang J, Li Y, Wang B T, Long S S, Li Q. Evaluation of resistance and detection of resistance gene(s) to stripe rust and powdery mildew of 194 wheat cultivars (lines) in China[J]. Acta Phytopathologica Sinica, 2025, 55(2): 261-269. (in Chinese)
[49]
Huang S, Zhang Y B, Ren H, Li X, Zhang X, Zhang Z Y, Zhang C L, Liu S J, Wang X T, Zeng Q D, Wang Q L, Singh R P, Bhavani S, Wu J H, Han D J, Kang Z S. Epistatic interaction effect between chromosome 1BL (Yr29) and a novel locus on 2AL facilitating resistance to stripe rust in Chinese wheat Changwu 357-9[J]. Theoretical and Applied Genetics, 2022, 135(7): 2501-2513.

doi: 10.1007/s00122-022-04133-9
[50]
李俣佳, 许豪, 于士男, 唐建卫, 李巧云, 高艳, 郑继周, 董纯豪, 袁雨豪, 郑天存, 殷贵鸿. 小麦骨干亲本周8425B抗条锈病优异基因在其衍生品种中的遗传解析[J]. 作物学报, 2024, 50(1): 16-31.

doi: 10.3724/SP.J.1006.2024.31013
Li Y J, Xu H, Yu S N, Tang J W, Li Q Y, Gao Y, Zheng J Z, Dong C H, Yuan Y H, Zheng T C, Yin G H. Genetic analysis of elite stripe rust resistance genes of founder parent Zhou 8425B in its derived varieties[J]. Acta Agronomica Sinica, 2024, 50(1): 16-31. (in Chinese)

doi: 10.3724/SP.J.1006.2024.31013
[51]
Hu Y L, Li M M, Li Y Q, Du L L, Xie R J, Ni F, Xia C J, Wang K, Huang Y Y, Xu B Y, Li Y H, Jiang Y F, Hao M, Jiang B, Ning S Z, Yuan Z W, Feng L H, Zhang L Q, Chen S S, Wu B H, et al. A head-to-head NLR gene pair from wild emmer confers stripe rust resistance in wheat[J]. Nature Genetics, 2025, 57(6): 1543-1552.

doi: 10.1038/s41588-025-02207-0
[52]
Hao M, Li Y Z, Fan C L, Chen H, Nie Y X, Wang F, Luo J T, Jiang B, Chen X, Chen X J, Yuan Z W, Ning S Z, Zhang L Q, Pu Z J, Cao D, Chen S S, Huang L, Liu B L, Liu D C. Exploiting phKL-mediated homoeologous recombinants to map stripe rust resistance gene YrAev from Aegilops variabilis[J]. The Crop Journal, 2025, 13(4): 1178-1185.

doi: 10.1016/j.cj.2025.05.015
[53]
Wang Y J, Abrouk M, Gourdoupis S, Koo D H, Karafiátová M, Molnár I, Holušová K, Doležel J, Athiyannan N, Cavalet-Giorsa E, Jaremko Ł, Poland J, Krattinger S G. An unusual tandem kinase fusion protein confers leaf rust resistance in wheat[J]. Nature Genetics, 2023, 55(6): 914-920.

doi: 10.1038/s41588-023-01401-2
[54]
Du X J, Hu L, Xu W G, Dong H B, Qi X L, Peng C J, Zhang Y, Li Z L. Mapping, validation, and development of markers for the major adult plant stripe rust resistance locus QYr.hnk-7DL in Henan wheat[J]. BMC Plant Biology, 2025, 25(1): 808.

doi: 10.1186/s12870-025-06918-8
[55]
Sapkota S, Hao Y F, Johnson J, Buck J, Aoun M, Mergoum M. Genome-wide association study of a worldwide collection of wheat genotypes reveals novel quantitative trait loci for leaf rust resistance[J]. The Plant Genome, 2019, 12(3): 1-14.
[56]
Athiyannan N, Abrouk M, Boshoff W H P, Cauet S, Rodde N, Kudrna D, Mohammed N, Bettgenhaeuser J, Botha K S, Derman S S, Wing R A, Prins R, Krattinger S G. Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning[J]. Nature Genetics, 2022, 54(3): 227-231.

doi: 10.1038/s41588-022-01022-1 pmid: 35288708
[57]
Jiang C, Wang X S, Chen W Q, Liu T G, Zhong S F, Huang Q L, Ren T H, Li Z, Tan F Q, Luo P G. Resistance performance of wheat stripe rust resistance gene Yr41 and its effect on yield parameters in F2 populations under field conditions[J]. Crop Protection, 2020, 134: 105168.

doi: 10.1016/j.cropro.2020.105168
[58]
Cheng P, Chen X M. Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s[J]. Theoretical and Applied Genetics, 2010, 121(1): 195-204.

doi: 10.1007/s00122-010-1302-0 pmid: 20198466
[59]
Xu L S, Wang M N, Cheng P, Kang Z S, Hulbert S H, Chen X M. Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat[J]. Theoretical and Applied Genetics, 2013, 126(2): 523-533.

doi: 10.1007/s00122-012-1998-0 pmid: 23090143
[60]
Dracatos P M, Zhang P, Park R F, McIntosh R A, Wellings C R. Complementary resistance genes in wheat selection ‘Avocet R’ confer resistance to stripe rust[J]. Theoretical and Applied Genetics, 2016, 129(1): 65-76.

doi: 10.1007/s00122-015-2609-7 pmid: 26433828
[61]
Chen X M. Gene number and heritability of wheat cultivars with durable, high-temperature, adult-plant (HTAP) resistance and interaction of HTAP and race-specific seedling resistance toPuccinia striiformis[J]. Phytopathology, 1996, 85(5): 573.

doi: 10.1094/Phyto-85-573
[62]
Bansal U K, Forrest K L, Hayden M J, Miah H, Singh D, Bariana H S. Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52[J]. Theoretical and Applied Genetics, 2011, 122(8): 1461-1466.

doi: 10.1007/s00122-011-1545-4 pmid: 21344185
[63]
Bansal M, Adamski N M, Toor P I, Kaur S, Molnár I, Holušová K, Vrána J, Doležel J, Valárik M, Uauy C, Chhuneja P. Aegilops umbellulata introgression carrying leaf rust and stripe rust resistance genes Lr76 and Yr70 located to 9.47-Mb region on 5DS telomeric end through a combination of chromosome sorting and sequencing[J]. Theoretical and Applied Genetics, 2020, 133(3): 903-915.

doi: 10.1007/s00122-019-03514-x
[64]
Kanwal M, Qureshi N, Gessese M, Forrest K, Babu P, Bariana H, Bansal U. An adult plant stripe rust resistance gene maps on chromosome 7A of Australian wheat cultivar Axe[J]. Theoretical and Applied Genetics, 2021, 134(7): 2213-2220.

doi: 10.1007/s00122-021-03818-x pmid: 33839800
[65]
Zwart R S, Thompson J P, Milgate A W, Bansal U K, Williamson P M, Raman H, Bariana H S. QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat[J]. Molecular Breeding, 2010, 26(1): 107-124.

doi: 10.1007/s11032-009-9381-9
[66]
Liu W Z, Maccaferri M, Chen X M, Laghetti G, Pignone D, Pumphrey M, Tuberosa R. Genome-wide association mapping reveals a rich genetic architecture of stripe rust resistance loci in emmer wheat (Triticum turgidum ssp. dicoccum)[J]. Theoretical and Applied Genetics, 2017, 130(11): 2249-2270.

doi: 10.1007/s00122-017-2957-6
[67]
杨芳萍, 刘金栋, 郭莹, 贾奥琳, 闻伟鄂, 巢凯翔, 伍玲, 岳维云, 董亚超, 夏先春. 普通小麦'Holdfast'条锈病成株抗性QTL定位[J]. 作物学报, 2019, 45(12): 1832-1840.

doi: 10.3724/SP.J.1006.2019.91026
Yang F P, Liu J D, Guo Y, Jia A L, Wen W E, Chao K X, Wu L, Yue W Y, Dong Y C, Xia X C. QTL mapping of adult-plant resistance to stripe rust in wheat variety holdfast[J]. Acta Agronomica Sinica, 2019, 45(12): 1832-1840. (in Chinese)

doi: 10.3724/SP.J.1006.2019.91026
[68]
Bremenkamp-Barrett B, Faris J D, Fellers J P. Molecular mapping of the leaf rust resistance gene Lr17a in wheat[J]. Crop Science, 2008, 48(3): 1124-1128.

doi: 10.2135/cropsci2007.07.0379
[69]
Naik B K Vinod, Sharma J B, Sivasamy M, Prabhu K V, Tomar R S, Tomar S M S. Molecular mapping and validation of the microsatellite markers linked to the Secale cereale-derived leaf rust resistance gene Lr45 in wheat[J]. Molecular Breeding, 2015, 35(2): 61.

doi: 10.1007/s11032-015-0234-4
[70]
Zhang Q, Wei W X, Zuansun X X, Zhang S N, Wang C, Liu N N, Qiu L N, Wang W D, Guo W L, Ma J, Peng H R, Hu Z R, Sun Q X, Xie C J. Fine mapping of the leaf rust resistance gene Lr65 in spelt wheat ‘altgold’[J]. Frontiers in Plant Science, 2021, 12: 666921.

doi: 10.3389/fpls.2021.666921
[71]
Bipinraj A, Honrao B, Prashar M, Bhardwaj S, Rao S, Tamhankar S. Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat[J]. Journal of Applied Genetics, 2011, 52(2): 171-175.

doi: 10.1007/s13353-010-0026-9
[72]
Yang J W, Li H N, Li M Y, Song R, Shen T, Wang G P, Xu D, Hao M, Jia A L, Rehman S U, Hua L, Liang Y Y, Chi C, Lan C X, Deng X W, Dubcovsky J, Song B X, Wang X D, Chen S S. Genome-assisted identification of wheat leaf rust resistance gene Lr.ace-4A/Lr30[J]. Nature Communications, 2025, 16: 9339.

doi: 10.1038/s41467-025-64428-5
[1] YE MeJin, WU Lei, MD NAHIBUZZAMAN Lohani, YIN Li, HU XinRong, LIU YaXi, JIANG YunFeng, CHEN GuoYue, PU ZhiEn, LI Yang, LI Ting, ZOU YaYa, WU JiaYi, MA Jian. Genome-Wide Association Study-Based Identification of Loci Controlling Mature Embryo Size in Chinese Wheat Landraces and Their Genetic Effects Analysis [J]. Scientia Agricultura Sinica, 2026, 59(6): 1157-1171.
[2] YANG LiJuan, CHEN SiYu, ZHAO Wei, ZHU Ling, GUO Lei, MA LiNa, MA RuiMin, ZHANG Juan. Whole-Genome Resequencing Reveals the Genetic Mechanisms Underlying Feather Coloration in Jingyuan Chicken [J]. Scientia Agricultura Sinica, 2026, 59(6): 1348-1360.
[3] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[4] WANG YongSheng, NIU Li, WANG ChangJie, MA LiHua, LIAN XiaoXiao, MENG YaXiong, MA XiaoLe, YAO LiRong, ZHANG Hong, YANG Ke, LI BaoChun, WANG HuaJun, SI ErJing, WANG JunCheng. Genome-Wide Association Study and Candidate Gene Identification for Thousand Grain Weight in Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 499-514.
[5] TAN YaNing, ZHANG BaoLong, CAO AiZhong, REN RunSheng. Molecular Detection of Disease Resistance Genes in Wheat Varieties Approved in Jiangsu Province [J]. Scientia Agricultura Sinica, 2026, 59(13): 2763-2775.
[6] YE MeiJin, WANG TongZhu, CHEN Bin, LOHANI Md Nahibuzzaman, CHEN JiaTing, HU XinRong, YIN Li, WANG Chao, ZHANG HaoPeng, YANG Xia, WANG JiaLin, YAO QiFu, DING PuYang, WANG Feng, LI XiaoYu, MA Jian. Genetic Dissection of Stem Internode Length and Its Effects in Wheat Based on a Genome-Wide Association Study [J]. Scientia Agricultura Sinica, 2026, 59(12): 2551-2562.
[7] WANG HuiLing, WANG XiaoYue, YAN AiLing, LIU ZhenHua, REN JianCheng, LU HaoCheng, SUN Lei. Research Progress in Genetic Characteristics and Loci Mapping for Grape Berry Quality Traits [J]. Scientia Agricultura Sinica, 2026, 59(11): 2447-2467.
[8] WU Yu, QU XiangRu, YANG Dan, WU Qin, CHEN GuoYue, JIANG QianTao, WEI YuMing, XU Qiang. Widespread Non-Targeted Metabolomics Reveals Metabolites of Chloroplasts in Wheat Responses to Stripe Rust [J]. Scientia Agricultura Sinica, 2025, 58(7): 1333-1343.
[9] ZOU XiaoWei, XIA Lei, ZHU XiaoMin, SUN Hui, ZHOU Qi, QI Ji, ZHANG YaFeng, ZHENG Yan, JIANG ZhaoYuan. Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2025, 58(6): 1116-1130.
[10] XU YuJuan, ZHANG Jie, WANG TianYi, CHEN HaoYang, ZHAO JiaJia, WU BangBang, HAO YuQiong, LI XiaoHua, ZHENG XingWei, ZUO JingJing, ZHENG Jun. Identification of Glu-A3 and Glu-B3 of Low-Molecular-Weight Glutenin in Shanxi Wheat and Its Effect on Quality [J]. Scientia Agricultura Sinica, 2025, 58(24): 5110-5127.
[11] LI Ming, CHENG YuKun, BAI Bin, LEI Bin, GENG HongWei. Genome-Wide Association Study on Spike Architecture Traits and Elite Haplotype Mining in Winter Wheat [J]. Scientia Agricultura Sinica, 2025, 58(18): 3583-3597.
[12] XIANG AiHui, BAI RongJi, HAO YuQiong, ZHAO JiaJia, WU BangBang, LI XiaoHua, ZHENG XingWei, GUAN PanFeng, ZHENG Jun. Identification of Dwarf Genes and Mining of Plant Height Genetic Loci in Shanxi Wheat [J]. Scientia Agricultura Sinica, 2025, 58(17): 3372-3388.
[13] LIU HongXiang, ZHANG XuePing, WANG YiFei, WANG ZhiCheng, GU HaoTian, SONG WeiTao, TAO ZhiYun, XU WenJuan, ZHANG ShuangJie, LU LiZhi, LI HuiFang, ZHU ChunHong. Genome-Wide Association Study of Egg Production Traits in Jinding Duck [J]. Scientia Agricultura Sinica, 2025, 58(15): 3145-3158.
[14] XIE HuiHui, YANG QiuHua, LI WenLi, ZHU JinCheng, LI HuiXia, ZHANG Feng. Identification of Wild Potato Introgression Lines Resistant to Southern Root-Knot Nematode [J]. Scientia Agricultura Sinica, 2025, 58(14): 2924-2932.
[15] ZHAO ZhenJian, WANG Kai, CHEN Dong, SHEN Qi, YU Yang, CUI ShengDi, WANG JunGe, CHEN ZiYang, YU ShiXin, CHEN JiaMiao, WANG XiangFeng, TANG GuoQing. Integrated Aanalysis of Genome and DNA Methylation for Screening Key Genes Related to Pork Quality Traits [J]. Scientia Agricultura Sinica, 2024, 57(7): 1394-1406.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd