Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (24): 5125-5134.doi: 10.3864/j.issn.0578-1752.2020.24.015

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles    

Optimized Promoter Regulating of Duck Tembusu Virus E Protein Expression Delivered by a Vectored Duck Enteritis Virus in vitro

CHEN Liu(),NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun()   

  1. Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021
  • Received:2020-01-17 Accepted:2020-07-29 Online:2020-12-16 Published:2020-12-28
  • Contact: Cun ZHANG E-mail:haoliuzi@126.com;zhangcun@aliyun.com

RichHTML

22

PDF

675

Abstract:

【Background】 Duck enteritis virus (DEV) and duck Tembusu virus (DTMUV) are considered to be two of the important viruses that infected ducks. DEV is classified into the family Herpesviridae, which has the characterization of live viral vector. 【Objective】In our previous study, a recombinant DEV delivering optimized DTMUV E451 gene (E451-dk) referring to duck’s codon usage bias has been selected. In this study, the promoter regulating E451-dk (Es in short) in rDEV-EF1 was also evaluated for enhancing E451-dk expression level. 【Method】 The transfer vector pEP-BGH-pro-Es were constructed by separately substituted pCMV (cytomegalovirus major immediate-early promoter) on the vector pEP-BGH-Es with pCAG (human cytomegalovirus enhancer and chicken-actin promoter), pSV40 (the simian virus 40 promotor), pRSV(Rous sarcoma virus (RSV) promoter), pgB（MDV）(marek's disease virus (MDV) gB gene promoter) and p1.8k（MDV）(MDV 1.8k gene promoter). The recombinant DEV BAC clone pDEV-pro-Es carrying pro-Es genes were generated by two-step Red E/T recombination in E. coli. pDEV-pro-Es were constructed by inserting pro-Es expression cassette between DEV US7 and US8 genes on the infectious clone of DEV (pDEV-EF1). The recombinant virus rDEV-pro-Es (rDEV-pCAG-Es, rDEV-pSV40-Es, rDEV-pRSV-Es, rDEV-pgB(MDV)-Es and rDEV-p1.8k(MDV)-Es) were rescued from chicken embryo fibroblasts (CEFs) by calcium phosphate precipitation. The plaque size and expression of DTMUV Es in recombinant virus-infected CEFs were analyzed. 【Result】 All viruses were successfully rescued from CEFs. Western blot analysis showed that the expression level of Es in rDEV-pRSV-Es -infected cells was increased 169.12% compared to that of rDEV-Es -infected cells. 【Conclusion】pRSV was the highest effective promoter chosen in this study which regulating Es expression on recombinant DEV genome backbone. These studies laid a foundation for developing bivalent vaccine controlling DEV and DTMUV infection.

Key words: duck enteritis virus, duck Tembusu virus, E protein, bacterial artificial chromosome, viral vector, promoter

Table 1

Primers used in this study"

引物名称 Primer 序列 Sequence 引入位点 Sequence introduced
pCAG(MluI+) 5′-cgACGCGTTAGTTATTAATAGTAATCAATTACG-3′ Mlu I
PCAG(NheI-) 5′-ctaGCTAGCGCCGCCGGTCACACGCCAGAAGCC-3′ Nhe I
pRSV(BglII+) 5′-gaAGATCTCTGCTCCCTGCTTGTGTGTTG-3′ Bgl II
pRSV(NheI-) 5′-ctaGCTAGCGTGCACACCAATGTGGTGAATG-3′ Nhe I
pSV40(MluI+) 5′-cgACGCGTCTGTGGAATGTGTGTCAGTTAGG-3′ Mlu I
pSV40(NheI-) 5′-ctaGCTAGCCGAAAATGGATATACAAGCTCCCGG-3′ Nhe I
F(MDV p1.8k BglII+) 5′-gaAGATCTTCGAGGCCACAAGAAATTAC-3′ Bgl II
R(MDV p1.8k NheI-) 5′-ctaGCTAGCGAGCATCGCGAAGAGAGAAG-3′ Nhe I
F(MDV gB BglII+) 5'-cgAGATCTCAAGTCTCACTCACAAATTTTTTC-3′ Bgl II
R(MDV gB NheI-) 5′-ctaGCTAGCAGTGAGATGATCTTAATGATGC-3′ Nhe I
pDEV vac-in-s(p1.8k,pgB) 5′-TACTAATTTAAGTGTGCAGCCTGGTTAACTGTATTATGCGCGGAGTGACGTCGACGGATCGGG-3′
pDEV vac-in-s 5′-TACTAATTTAAGTGTGCAGCCTGGTTAACTGTATTATGCGCGGAGCGATGTACGGGCCAGATA-3′
pDEV vac-in-as 5′- TCCGTAGTCTGGCCGGCAGTATGTTGGTGTTTAGTACTCCAAACCCA TAGAGCCCACCGCATCCCC-3′
JD-F 5′-CTACCACAAGCGTCATCAACCA-3′
JD-R 5′-TGTCCATTACCAAATCCGAAAA-3′
DEV-tk-F 5′- GCTTCCCAGCAGCTCGTT-3′
DEV-tk-R 5′- TCTCGTACTTCAGCGGCACA-3′
Dev UL44(BamHI+) 5′-cgGGATCCATGGGGCCATTAGTGATGGTTG-3′ BamH I
Dev UL44 (XhoI-) 5′-ccgCTCGAGTCAAATAATATTGTCTGCTTTATC-3′ XhoI

Fig. 1

Analysis of recombinant BAC mutants by Pst I digestion 1: pDEV-EF1; 2: pDEV-Es; 3: pDEV-kan. pRSV. Es; 4: pDEV-pRSV. Es; 5: pDEV-kan.pSV40. Es; 6: pDEV-pSV40. Es; 7: pDEV-kan.pCAG. Es; 8: pDEV-pCAG. Es; 9: pDEV-kan.p1.8k(MDV). Es; 10: pDEV-p1.8k (MDV). Es; 11: pDEV-kan.pgB(MDV). Es; 12: pDEV-pgB(MDV). Es"

Fig. 2

Analysis of recombinant BAC mutants by PCR amplification"

Fig. 3

The rescued viruses (100×)"

Fig. 4

Plaque area measurement of recombinant viruses on CEFs"

Fig. 5

Western blotting analysis of Es protein expressed in recombinant virus-infected CEFs 1: rDEV-Es; 2: rDEV-pSV40-Es; 3: rDEV-pCAG-Es; 4: rDEV-pRSV-Es; 5: rDEV-p1.8(MDV) -Es; 6: rDEV-pgB(MDV)-Es; M: EasySee Western Marker (90, 75, 60,40, 25 kD); 7: rDEV-EF1"

[1] KALODIMOU G, VEIT S, JANY S, KALINKE U, BRODER C C, SUTTER G, VOLZ A. A soluble version of nipah virus glycoprotein G delivered by vaccinia virus MVA activates specific CD8 and CD4 T cells in mice. Viruses, 2020,12(1):26.
doi: 10.3390/v12010026
[2] BERTRAN K, CRIADO M F, LEE D H, KILLMASTER L, Sà E SILVA M, LUCIO E, WIDENER J, PRITCHARD N, ATKINS E, MEBATSION T, SWAYNE D E. Protection of White Leghorn chickens by recombinant fowlpox vector vaccine with an updated H5 insert against Mexican H5N2 avian influenza viruses. Vaccine, 2020,38(6):1526-1534.
doi: 10.1016/j.vaccine.2019.11.072 pmid: 31862196
[3] KIM J W, MORSHED R A, KANE J R, AUFFINGER B, QIAO J, LESNIAK M S. Viral vector production: adenovirus. Methods in Molecular Biology, 2016,1382:115-130.
doi: 10.1007/978-1-0716-0255-3_8 pmid: 32002905
[4] LAROCCA R A, MENDES E A, ABBINK P, PETERSON R L, MARTINOT A J, IAMPIETRO M J, KANG Z H, AID M, KIRILOVA M, JACOB-DOLAN C, TOSTANOSKI L, BORDUCCHI E N, De La BARRERA R A, BAROUCH D H. Adenovirus vector- based vaccines confer maternal-fetal protection against Zika virus challenge in pregnant IFN-αβR-/- mice. Cell Host &Microbe, 2019,26(5):591-600.
doi: 10.1016/j.chom.2019.10.001 pmid: 31668877
[5] CHANG P, AMEEN F, SEALY J E, SADEYEN J R, BHAT S, LI Y, IQBAL M. Application of HDR-CRISPR/Cas9 and erythrocyte binding for rapid generation of recombinant turkey herpesvirus-vectored avian influenza virus vaccines. Vaccines (Basel), 2019,7(4):E192.
[6] ŚMIETANKA K, TYBOROWSKA J, OLSZEWSKA-TOMCZYK M, DOMAńSKA-BLICHARZ K, MINTA Z, RABALSKI L, CZARNOTA A, KUCHARCZYK K, SZEWCZYK B. A Recombinant turkey herpesvirus expressing F and HN genes of avian avulavirus-1 (AAvV-1) genotype VI confers cross-protection against challenge with virulent AAvV-1 genotypes IV and VII in chickens. Viruses, 2019,11(9):E784.
doi: 10.3390/v11090784 pmid: 31450681
[7] KAMEL M, El-SAYED A. Utilization of herpesviridae as recombinant viral vectors in vaccine development against animal pathogens. Virus Research, 2019,270:197648.
doi: 10.1016/j.virusres.2019.197648 pmid: 31279828
[8] LEMIALE F, ASEFA B, YE D, CHEN C, KOROKHOV N, HUMEAU L. An HIV-based lentiviral vector as HIV vaccine candidate: immunogenic characterization. Vaccine, 2010,28(8):1952-1961.
doi: 10.1016/j.vaccine.2009.10.089
[9] LIN A, BALAZS A B. Adeno-associated virus gene delivery of broadly neutralizing antibodies as prevention and therapy against HIV-1. Retrovirology, 2018,15(1):66.
doi: 10.1186/s12977-018-0449-7 pmid: 30285769
[10] 展小过, 乔传玲, 杨焕良, 陈艳, 孔维, 辛晓光, 陈化兰. 表达H3N2亚型猪流感病毒HA基因重组腺病毒对小鼠免疫原性的研究. 中国农业科学, 2010,43(6):1235-1241.
ZHAN X G, QIAO C L, YANG H L, CHEN Y, KONG W, XIN X G, CHEN H L. Immunogenicity of a recombinant adenovirus expressing HA gene of H3N2 subtype swine influenza virus in mice. Scientia Agricultura Sinica, 2010,43(6):1235-1241. (in Chinese)
[11] 陈化兰, 马文军, 于康震. 表达禽流感病毒血凝素基因的重组禽痘病毒的构建, 中国农业科学, 2000,33(5):1-7.
CHEN H L, MA W J, YU K Z. Construction of a recombinant fowlpox virus expressing hemagglutinin gene of avian influenza virus. Scientia Agricultura Sinica, 2000,33(5):1-7. (in Chinese)
[12] CHEN P, DING L, JIANG Y, ZENG X, DENG G, SHI J, LI Y, LIU L, ZHAO Y, HU Y, LIU J, CHEN H. Protective efficacy in farmed ducks of a duck enteritis virus-vectored vaccine against H5N1, H5N6, and H5N8 avian influenza viruses. Vaccine, 2019,37(40):5925-5929.
doi: 10.1016/j.vaccine.2019.08.026 pmid: 31471151
[13] DING L, CHEN P, BAO X, LI A, JIANG Y, HU Y, GE J, ZHAO Y, WANG B, LIU J, CHEN H. Recombinant duck enteritis viruses expressing the Newcastle disease virus (NDV) F gene protects chickens from lethal NDV challenge. Veterinary Microbiology, 2019,232:146-150.
doi: 10.1016/j.vetmic.2019.04.022 pmid: 31030839
[14] CHANG P, YAO Y, TANG N, SADEYEN J R, SEALY J, CLEMENTS A, BHAT S, MUNIR M, BRYABT J E, IQBAL M. the application of nhej-crispr/cas9 and cre-lox system in the generation of bivalent duck enteritis virus vaccine against avian influenza virus. Viruses, 2018,10(2):E81.
doi: 10.3390/v10020081 pmid: 29438322
[15] ZOU Z, HUANG K, WEI Y, CHEN H, LIU Z, JIN M. Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus- based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection. Scientific Reports, 2017,7(1):1478.
doi: 10.1038/s41598-017-01554-1 pmid: 28469192
[16] ZOU Z, MA J, HUANG K, CHEN H, LIU Z, JIN M. Live attenuated vaccine based on duck enteritis virus against duck hepatitis a virus types 1 and 3. Frontiers in Microbiology, 2016,7:1613.
doi: 10.3389/fmicb.2016.01613 pmid: 27777571
[17] SUN Y, YANG C, LI J, LI L, CAO M, LI Q, LI H. Construction of a recombinant duck enteritis virus vaccine expressing hemagglutinin of H9N2 avian influenza virus and evaluation of its efficacy in ducks. Archives of Virology, 2017,162(1):171-179.
doi: 10.1007/s00705-016-3077-3 pmid: 27709401
[18] LI H, WANG Y, HAN Z, WANG Y, LIANG S, JIANG L, HU Y, KONG X, LIU S. Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Antiviral Research, 2016,130:19-26.
doi: 10.1016/j.antiviral.2016.03.003 pmid: 26946113
[19] WANG J, GE A, XU M, WANG Z, QIAO Y, GU Y, LIU C, LIU Y, HOU J. Construction of a recombinant duck enteritis virus (DEV) expressing hemagglutinin of H5N1 avian influenza virus based on an infectious clone of DEV vaccine strain and evaluation of its efficacy in ducks and chickens. Virology Journal, 2015,12:126.
doi: 10.1186/s12985-015-0354-9 pmid: 26263920
[20] ZOU Z, HU Y, LIU Z, ZHONG W, CAO H, CHEN H, JIN M. Efficient strategy for constructing duck enteritis virus-based live attenuated vaccine against homologous and heterologous H5N1 avian influenza virus and duck enteritis virus infection. Veterinary Research, 2015,46:42.
doi: 10.1186/s13567-015-0174-3 pmid: 25889564
[21] WANG J, OSTERRIEDER N. Generation of an infectious clone of duck enteritis virus (DEV) and of a vectored DEV expressing hemagglutinin of H5N1 avian influenza virus. Virus Research, 2011,159(1):23-31.
doi: 10.1016/j.virusres.2011.04.013
[22] LIU J, CHEN P, JIANG Y, WU L, ZENG X, TIAN G, GE J, KAWAOKA Y, BU Z, CHEN H. A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks. Journal of Virology, 2011,85(21):10989-10998.
doi: 10.1128/JVI.05420-11
[23] LIU X, WEI S, LIU Y, FU P, GAO M, MU X, LIU H, XING M, MA B, WANG J. Recombinant duck enteritis virus expressing the HA gene from goose H5 subtype avian influenza virus. Vaccine, 2013,31(50):5953-5959.
doi: 10.1016/j.vaccine.2013.10.035 pmid: 24144474
[24] CHEN P, LIU J, JIANG Y, ZHAO Y, LI Q, WU L, HE X, CHEN H. The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck Tembusu virus. Vaccine, 2014,32(41):5271-5277.
doi: 10.1016/j.vaccine.2014.07.082
[25] ZOU Z, LIU Z, JIN M. Efficient strategy to generate a vectored duck enteritis virus delivering envelope of duck Tembusu virus. Viruses, 2014,6(6):2428-2443.
doi: 10.3390/v6062428 pmid: 24956180
[26] 陈柳, 余斌, 倪征, 华炯钢, 叶伟成, 云涛, 张存. 表达小鹅瘟病毒VP2蛋白重组鸭瘟病毒的构建及其生物学特性. 中国农业科学, 2016,49(14):2813-2821.
doi: 10.3864/j.issn.0578-1752.2016.14.015
CHEN L, YUN B, NI Z, HUA J G, YE W C, YUN T, ZHANG C. Construction and characterization of a recombinant duck enteritis virus expressing VP2 gene of goose parvovirus. Scientia Agricultura Sinica, 2016, 49(14):2813-2821. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2016.14.015
[27] LI K, LIU Y, LIU C, GAO L, ZHANG Y, GAO Y, CUI H, QI X, ZHONG L, WANG X. Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious Bursal disease virus. Vaccine, 2016,34(47):5744-5750.
doi: 10.1016/j.vaccine.2016.10.008 pmid: 27742216
[28] CHEN L, YU B, HUA J, et al. Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain. Virology Journal, 2013,10:328.
doi: 10.1186/1743-422X-10-328 pmid: 24195756
[29] 陈柳, 余斌, 倪征, 华炯钢, 叶伟成, 云涛, 张存. 表达鸭坦布苏病毒E 蛋白的重组鸭瘟病毒的构建及其生物学特性. 浙江农业学报, 2015,27(11):1889-1895.
CHEN L, YU B, NI Z, HUA J G, YE W C, YUN T, ZHANG C. Construction and characterization of a recombinant duck enteritis virus expressing E protein of duck Tembusu virus. Acta Agriculturae Zhejiangeensis, 2015,27(11):1889-1895.(in Chinese)
[30] CHEN L, YU B, HUA J, NI Z, YE W, YUN T, ZHANG C. Optimized expression of duck Tembusu virus e gene delivered by a vectored duck enteritis virus in vitro. Molecular Biotechnology, 2019,61(10):783-790.
doi: 10.1007/s12033-019-00206-1 pmid: 31482466
[31] 马兴树. 禽传染病试验诊断技术. 北京: 化学工业出版社, 2006.
MA X S. Diagnostic Technique of Avian Infectious Disease. Beijing: Chemical Industry Press, 2006.(in Chinese)
[32] 余斌, 华炯钢, 刘跃生, 赵灵燕, 倪征, 叶伟成, 云涛, 陈柳, 徐辉, 张存. 鸭坦布苏病毒抗体间接ELISA 检测方法的建立和应用. 浙江畜牧兽医, 2014,5 : 1-6.
YU B, HUA J G, LIU Y S, ZHAO L Y, NI Z, YE W C, YUN T, CHEN L, XU H, ZHANG C. Establishment and application of indirect ELISA for detection of duck Tembusu virus antibody. Zhejiang Journal Animal Science and Veterinary Medicine, 2014,5:1-6. (in Chinese)
[33] TISCHER B K, von EINEM J, KAUFER B, OSTERRIEDER N. Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. Biotechniques, 2006,40(2):191-197.
doi: 10.2144/000112096 pmid: 16526409
[34] TISCHER B K, SMITH G A, OSTERRIEDER N. En passant mutagenesis: a two step markerless red recombination system. Jeff Braman(ed.), In Vitro Mutagenesis Protocls: Third Edition, Methods in Molecular Biology, 2010,634:421-430.
[35] TSUKAMOTO K, SAITO S, SAEKI S, SATO T, TANIMURA N, ISOBE T, MASE M, IMADA T, YUASA N, YAMAGUCHI S. Complete, long-lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens. Journal of Virology, 2002,76(11):5637-5645.
doi: 10.1128/jvi.76.11.5637-5645.2002 pmid: 11991992
[36] MA C, ZHANG Z, ZHAO P, DUAN L, ZHANG Y, ZHANG F, CHEN W, CUI Z. Comparative transcriptional activity of five promoters in BAC-cloned MDV for the expression of the hemagglutinin gene of H9N2 avian influenza virus. Journal of Virological Methods, 2014,206:119-127.
doi: 10.1016/j.jviromet.2014.05.023
[37] LI L, ZHANG Y, DONG J, ZHANG J, ZHANG C, SUN M, CAO Y. The truncated E protein of DTMUV provide protection in young ducks. Veterinary Microbiology, 2020,240:108508.
doi: 10.1016/j.vetmic.2019.108508 pmid: 31902493
[1] LIU LuPing, HU XueJie, QI Jin, CHEN Qiang, LIU Zhi, ZHAO TianTian, SHI XiaoLei, LIU BingQiang, MENG QingMin, ZHANG MengChen, HAN TianFu, YANG ChunYan. Cloning of the Promoters and Analysis of Expression Patterns of Maturity Genes E1 and E2 in Soybean [J]. Scientia Agricultura Sinica, 2025, 58(5): 840-850.
[2] LI NiFei, YANG QiaoMin, YANG KeCheng, XING YuTeng, WANG MengYuan, ZANG TianBao, LU MingHui. Functional Analysis of CaIAA8, An Interacting Protein of the Autophagy-Related Protein CaATG8c, in the Heat Tolerance of Pepper [J]. Scientia Agricultura Sinica, 2025, 58(22): 4732-4745.
[3] GUO TianFa, WU JinLong, QIU QianQian, MA XinChao, WANG LiRong, WU CuiYun. Relationship Between the Formation of Non-Red Color in the Fruit Skin of Xinjiang Local Peach Varieties and the Variation of PpMYB10.1 Promoter [J]. Scientia Agricultura Sinica, 2025, 58(2): 326-338.
[4] YU Zhe, ZHOU FangXue, LIU RunFa, TIAN YaQi, JIHAO MuHa, WANG YongXiang, FENG WenMi, MOU KeXin, JING Yan, LI HaiYan. Screening for Soybean Host Factors that Interact with Soybean Mosaic Virus Nuclear Inclusion Proteins Using the Yeast Two-Hybrid System [J]. Scientia Agricultura Sinica, 2025, 58(19): 3799-3813.
[5] YU YunYan, MA GaoXing, DUAN YaNing, TAO Qi, LI XinYi, HU QiuHui, MA Ning. The Potential of Alternative Proteins from Edible Fungi Based on Amino Acid and Physicochemical Characterization [J]. Scientia Agricultura Sinica, 2025, 58(15): 3097-3117.
[6] ZHAO QingYang, ZHANG XiaoXiao, GUO ChunHe. Construction and Evaluation of a PK15 Cell Line Stably Expressing Porcine CD163 Protein [J]. Scientia Agricultura Sinica, 2025, 58(11): 2253-2264.
[7] ZHANG CongYue, ZHOU Hong, LIN HuiXing, FAN HongJie. Development and Application of Indirect ELISA Kits for Antibody Detection of Porcine Proliferative Enteropathy [J]. Scientia Agricultura Sinica, 2024, 57(16): 3283-3293.
[8] QIN YuQi, SU JianXin, CAO XueMin, ZHU Wan, CHENG WenHan, ZHANG Wei. Cloning and Activity Analysis of U6 Promoter in Rosa chinensis Old Blush and Rosa multiflora [J]. Scientia Agricultura Sinica, 2024, 57(13): 2651-2661.
[9] CHEH ErHu, SHEN DanRong, DU WenWei, MENG HongJie, TANG PeiAn. Cuticle Protein Genes are Involved in Phosphine Resistance of Cryptolestes ferrugineus [J]. Scientia Agricultura Sinica, 2023, 56(9): 1696-1707.
[10] WEI YaNan, BO QiFei, TANG An, GAO JiaRui, MA Tian, WEI XiongXiong, ZHANG FangFang, ZHOU XiangLi, YUE ShanChao, LI ShiQing. Effects of Long-Term Film Mulching and Application of Organic Fertilizer on Yield and Quality of Spring Maize on the Loess Plateau [J]. Scientia Agricultura Sinica, 2023, 56(9): 1708-1717.
[11] YAO LiXiao, SU Juan, GUO XingRu, LI FengLong, HE YongRui, ZOU XiuPing, CHEN ShanChun. Cloning and Expression Analysis of PtMLP1 Promoter in Poncirus trifoliata [J]. Scientia Agricultura Sinica, 2023, 56(24): 4906-4915.
[12] LIU Fang, XU MengBei, WANG QiaoLing, MENG Qian, LI GuiMing, ZHANG HongJu, TIAN HuiDan, XU Fan, LUO Ming. Cloning and Functional Characterization of the Promoter of GhSLD1 Gene That Predominantly Expressed in Cotton Fiber [J]. Scientia Agricultura Sinica, 2023, 56(19): 3712-3722.
[13] XU FuChun, ZHAO JingRuo, ZHANG ZhenNan, HU GaiYuan, LONG Lu. Cloning and Functional Characterization of GhCPR5 in Disease Resistance of Gossypium hirsutum [J]. Scientia Agricultura Sinica, 2023, 56(19): 3747-3758.
[14] LI XuFei,YANG ShengDi,LI SongQi,LIU HaiNan,PEI MaoSong,WEI TongLu,GUO DaLong,YU YiHe. Analysis of VlCKX4 Expression Characteristics and Prediction of Transcriptional Regulation in Grape [J]. Scientia Agricultura Sinica, 2023, 56(1): 144-155.
[15] PEI YueHong,LI FengWei,LIU WeiNa,WEN YuXia,ZHU Xin,TIAN ShaoRui,FAN GuangJin,MA XiaoZhou,SUN XianChao. Characteristics of Cysteine Proteinase Gene Family in Nicotiana benthamiana and Its Function During TMV Infection [J]. Scientia Agricultura Sinica, 2022, 55(21): 4196-4210.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd