人工合成小麦SHW-L1高硒含量KASP分子标记开发及其应用

doi:10.3864/j.issn.0578-1752.2020.20.001

Abstract

Abstract:

【Objective】As one of the essential elements of the human body, selenium plays an important role in disease prevention and antioxidants. Wheat, as one of the main crops, is the main source for humans to obtain plant selenium sources, so increasing the selenium content of wheat grains is an efficient, inexpensive and easy way to supplement selenium. This study intends to develop KASP markers for the identified SNP loci for the breeding of high-selenium wheat.【Method】Using the completed 660K wheat SNP markers of synthetic wheat SHW-L1 recombinant inbred lines population ( recombinant inbred lines, RILs), scanning the RILs population, further narrowing the QTL interval related to the selenium content of the previously located grains, and developing the SNP loci in the stable gene as KASP markers.【Result】Two successfully developed KASP markers AX-1 and AX-2, markers can be used for genotyping and identification of selenium content in the grain of the material. Both markers can be used to classify test materials according to genotype into two categories, namely high-selenium and low-selenium materials, meets the 1:1 allele separation ratio. However, according to the phenotype, the screening rate (greater than 90%) for low-selenium materials is much higher than that of high-selenium materials (less than 30%), and it should be clear that 81% of the phenotype materials with high selenium content have high selenium genotypes, indicating the reliability of the label. Therefore, the KASP markers developed can be used to improve selection efficiency. At the same time, the KASP marker can also be used for genotyping in other derived offspring lines with artificial wheat SHW-L1 as the parent, and the typing results are consistent with the phenotypic results, indicating the validity of the marker.【Conclusion】AX-1 and AX-2 can be used as a practical molecular marker for breeding and germplasm resource creation of high selenium wheat varieties with SHW-L1 as the parent.

Key words: synthetic wheat, selenium content, KASP

WEI GuangHui,LI Zhi,CHEN Qiang,LI Yang,CHEN ShiHao,PEI Ying,ZHOU Yong,CHENG MengPing,TANG Hao,WANG JiRui,WEI YuMing,LIU DengCai,CHEN Li,ZHENG YouLiang,PU ZhiEn. Development and Utilization of KASP Marker for Se Concentration in Synthetic Wheat SHW-L1[J].Scientia Agricultura Sinica, 2020, 53(20): 4103-4112.

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References 39

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变异来源 Source of variation	平方和 Type Ⅲ SS	自由度 df	均方 MS	F值 F value	Sig.
V	0.02995031	114	0.00026272	13.410	**
T	0.00009015	1	0.00009015	0.4890
V×T	0.02100010	110	0.00019091
误差Error	0.00415800	110	0.00003780

籽粒硒含量 Grain selenium content	编号 Number	染色体 Chromosome	标记 Marker	位置 Position (cM)	R²	加性效应 Additive	LOD
Grain se-1	1	5A	22	43.62	0.0765	0.0003	2.5551
Grain se-2	1	5A	13	29.54	0.0654	0.0003	2.7476
Grain se-2	2	5A	16	32.66	0.0774	0.0003	3.4722
Grain se-2	3	5A	21	35.58	0.0877	0.0004	3.9723

编号 Number	染色体 Chromosome	变异碱基 Base	序列 Sequence (5′-3′)
AX-1	5A	A/G	CGTACCATGCGATCACACTTGGAGATGCGTGTGAG[A/G]GAAAAAGAGTACTCCATCCATCCAAATTCCATCCC
AX-2	5A	A/G	TGTAAATTAATTTGTTGTGGGTAGGGCATCTTGAG[A/G]AATGTTTACCTTCTTGCTGCTGCCTGGTTCGGTTT
AX-3	5A	A/C	GGGTGATAGCAAGGAAGCTGAAGAGCATGATGGAC[A/C]AGGGACAGACGGAAAAGAGTTGCAGGATGGACAAG
AX-4	5A	A/G	GATGACTTTGCGGCAATATTAAGAGATGTGCATCC[A/G]TTAGCAAGCAAGCTAGCGGACAAAACCAGCTTAAC
AX-5	5A	T/C	ACCCCTTGTTTAGTACCACCTCACCAAGTGAGAGT[C/T]ATTTCAACCGGTTTATAAGATTCAGCGTTCCAACC

品种或自交系 Cultivar or inbred lines	籽粒硒含量 Selenium content in grains (mg·kg^-1)	SNP基因分型 SNP genotyping
蜀麦969 Shumai 969	0.020	高硒High Se
蜀麦580 Shumai 580	0.011	低硒Low Se
蜀麦980 Shumai 980	0.009	低硒Low Se
蜀麦830 Shumai 830	0.017	高硒High Se
蜀麦114 Shumai 114	0.013	高硒 High Se

Development and Utilization of KASP Marker for Se Concentration in Synthetic Wheat SHW-L1

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