高硼土壤增施硫肥对油菜硼吸收与分配的影响

doi:10.3864/j.issn.0578-1752.2019.05.009

Abstract

Abstract:

【Objective】This study explored the effects of sulfur (S) fertilizer application on B uptake and distribution in rapeseed under high boron (B) environment, and provided a theoretical basis for rational application of S fertilizer to alleviate B toxicity in rapeseed (Brassica napus L.). 【Method】Rape plants (cv. Huayouza 9) were grown in pots filled with calcareous alluvial soil contaminated with B (total B content: 13.44 mg·kg ^-1; available B: 5.07 mg·kg ^-1) and the effects of S fertilizer level (0, 20, 50, 100, 200, and 500 mg·kg ^-1) on B concentration, distribution, cell wall extraction rate, cell wall B concentration and its proportion of total B in different parts of the rape were determined at the podding stage. 【Result】 With the increase of S application, the biomass increased at first and then decreased in different parts of rape. When the S was applied at 50 mg·kg ^-1, the fresh biomass of rape was the largest, reached 364.5 g/plant under different S application, and the B concentration and distribution in different parts of rape followed the order of pods>leaves>stems>roots, which indicated that the pods were the main accumulation site of B. As the S fertilizer level increased from 0 to 100 mg·kg ^-1, the pod B concentration and distribution ratios decreased by 14.8% and 15.0%, respectively. The extraction rate of cell wall of rapeseed pods increased by 43.0%, and there was no significant change in the B concentration and proportion in the cell wall. When S fertilizer was continuously applied to 200 mg·kg ^-1, the B concentration, distribution ratios and cell wall B concentration of rapeseed pods decreased significantly, and cell wall B proportion increased significantly compared with that of non-sulfur fertilizer. The opposite trends were found in the B concentrations and cumulative distribution ratios in the leaves, stems and roots. As the S fertilizer level increased from 0 to 100 mg·kg ^-1, the B concentration significantly increased by 15.0%, 32.9% and 34.9% in rape leaves, stems, and roots, respectively. The B distribution ratios significantly increased by 13.4%, 29.6% and 18.6%, and B concentration in the cell wall significantly increased by 12.3%, 22.9% and 14.9% in rape leaves, stems, and roots, respectively. It showed that the addition of S fertilizer increased the B concentration of the leaves, stems and roots of rapeseed. 【Conclusion】S fertilizer application effectively alleviated B toxicity by increasing biomass, limiting B translocation from roots, stems and leaves to pods, and by increasing the extraction rate of cell wall in rape plants grown on B-contaminated soil. The optimum amount of S fertilizer application was 50-100 mg·kg ^-1.

Key words: boron contamination, sulfur fertilizer, rape, boron distribution, cell wall

LI MingFeng,LIU XinWei,WANG HaiTong,ZHAO ZhuQing. Effects of Sulfur Fertilizer on Boron Uptake and Distribution of Rape in B-contaminated Soil[J].Scientia Agricultura Sinica, 2019, 52(5): 874-881.

Figures/Tables 5

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施硫量 S fertilizer level (mg·kg^-1)	油菜不同部位鲜重 Fresh weight in different parts of rape (g/plant)				单株生物量 Biomass (g/plant)
施硫量 S fertilizer level (mg·kg^-1)	果荚 Pod	叶 Leaf	茎 Stem	根 Root	单株生物量 Biomass (g/plant)
0	57.30±2.71c	77.02±6.46b	142.48±5.95cd	36.35±1.49ab	313.16±13.52cd
20	64.15±3.87abc	84.60±2.23ab	151.91±11.07bc	37.61±1.78a	338.26±13.33b
50	68.47±3.70a	90.90±5.38a	166.68±6.25a	38.49±2.18a	364.53±6.75a
100	64.57±5.62ab	85.79±7.10ab	157.18±7.58ab	38.76±2.65a	346.29±14.74ab
200	60.89±3.32bc	81.38±2.58ab	148.35±1.86bcd	35.72±1.82ab	326.34±5.23bc
500	57.71±2.15bc	77.00±3.93b	137.38±3.28d	33.42±2.51b	305.50±8.94d

施硫量 S fertilizer level (mg·kg^-1)	细胞壁提取率 The extraction rate of the cell wall (%)
施硫量 S fertilizer level (mg·kg^-1)	果荚 Pod	叶 Leaf	茎 Stem	根 Root
0	7.90±0.45c	7.65±0.32b	21.25±1.48a	20.71±1.18a
20	8.23±0.15c	7.62±0.43b	22.69±0.58a	19.77±1.20ab
50	9.25±0.17c	7.04±0.77ab	22.62±1.14a	19.36±0.77ab
100	11.30±0.97b	6.97±0.31ab	21.32±0.95a	18.44±1.00b
200	11.99±0.79ab	6.37±0.50a	19.20±1.18b	17.91±0.77b
500	12.94±1.41a	6.31±0.23a	18.95±0.67b	17.87±1.61b

Effects of Sulfur Fertilizer on Boron Uptake and Distribution of Rape in B-contaminated Soil

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