豫芝11号种子发育过程中蛋白质及其组分的变化规律

doi:10.3864/j.issn.0578-1752.2018.04.005

Abstract

Abstract: 【Objective】The purpose of this study was to explore the objective of sesame in growth period and the effect of surroundings on yield and constituent of sesame protein to provide reference for sesame breeding and protein application.【Method】Sesame seeds（Yuzhi 11） were planted in Nanyang, Zhumadian and Zhoukou of Henan province in the same growing season. About 5 000-flowers bloom at same time were marked in one day, and samples were acquired after ten days later and every five days then until sesame seeds mature. And then, content, constituent, relative molecular mass, and amino acid of sesame protein were measured.【Result】The content of sesame protein increased gradually with the growth period, and the protein content in sesame seeds reached the maximum at thirty-fifth days after pollination, and then the protein content was stable; The protein content of Zhoukou test site was lower than that in Nanyang and Zhumadian. The change of globulin was consistent with that of the total protein content of sesame, and the content of albumin showed the trend of increasing at first and then decreasing. The content of albumin in mature sesame seeds did not show regional difference; When the sesame seeds were pollinated for 15 days, bands appeared in gel chart at 20, 30 and 55 kD, and they grew deeper with the extension of the growth period. With the growth and development of sesame, the percentage of essential amino acids in total amino acids decreased gradually, and the proportion of essential amino acids in Nanyang and Zhumadian test site lower than that in Zhoukou significantly; Valine, isoleucine, leucine and phenylalanine + tyrosine ratio coefficients were greater than 1.【Conclusion】Surrounding has a significant effect on sesame protein content, globulin content and albumin content of sesame in growth period. Protein molecules synthesis in different time from kinds, and the main relative molecule weight is in 20-30 kD. With the growth and development of sesame, the percentage of essential amino acids in total amino acids decreased gradually, which indicates that sesame protein could be supplement on valine, isoleucine, leucine and phenylalanine and tryosine.

Key words: sesame, seed growth, protein, protein component, amino acid composition

HAN YaFei, WANG XueDe, ZHENG YongZhan, MEI HongXian, WEI AnChi, LIU YanYang. Study on Changes of Sesame Protein Content and Its Components of Yuzhi 11 Sesame Seed During Growth Period[J].Scientia Agricultura Sinica, 2018, 51(4): 652-661.

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