基于EST-SSR标记甘薯连锁图谱构建及淀粉性状的QTL定位

doi:10.3864/j.issn.0578-1752.2016.23.003

Abstract

Abstract: 【Objective】To provide a theoretical and practical basis for utilization of germplasm resources of sweetpotato with high starch content and molecular marker-assisted selection in sweetpotato, a genetic linkage map was constructed by using molecular markers, and quantitative trait loci (QTLs) associated with starch content were identified. 【Method】 A F₁ hybrid population was developed from a cross between Wanshu 5, a sweetpotato cultivar with high starch content, as the female parent, and Shangqiu 52-7, a cultivar with low starch content, as the male parent. By using EST-SSR markers and the software of JoinMap4.0, the molecular genetic linkage maps of both parents were constructed based on the “double pseudo testcross” strategy, respectively. And QTLs for starch content trait were identified using composite interval mapping method based on the phenotypic data of F₁ population in two years (2012 and 2013).【Result】Among the 1 679 EST-SSR primer pairs primarily tested in F₁ lines, 1 045 polymorphic primer pairs were selected to screen in the population lines, and 1 418 polymorphic loci were obtained. The genetic linkage relationship of polymorphic loci were analyzed with the LOD≥5.0 as a threshold, and the genetic linkage map of female and male parents were constructed respectively. A total of 74 linkage groups for female parent were constructed based on 642 polymorphic loci, of which 215 (33.5%) loci were placed on the genetic linkage map. The number of markers in each linkage group ranged from 2 to 11. The length of linkage groups ranged from 2.0 to 156.8 cM, and the linkage map covered a total length of 3 826.07 cM, with an average distance between markers of 17.80 cM. A genetic linkage map for male Shangqiu52-7 was constructed by using 776 polymorphic loci, of which 250 (32.5%) loci distributed on 80 linkage groups. The number of markers in each linkage group ranged from 2 to 24. The length of linkage groups ranged from 2.0 to 156.8 cM, and the linkage map covered a whole length of 3 955.0 cM with an average interval of 15.7 cM between markers. Using QTL analysis software Map QTL 5.0 and Interval Mapping method, QTLs for starch content were identified based on the starch content measured in two years (2012 and 2013) and two environments. Seventeen QTLs for starch content were detected, explaining 8.4%-40.5% of the phenotypic variation. Three QTLs qWsc-1, qWsc-2, and qWsc-3 mapped on the linkage group of female Wanshu 5 were detected under two environments. Fourteen QTLs were detected on the linkage group of male Shangqiu52-7, of which qSsc-1, qSsc-2, qSsc-3, qSsc-4, qSsc-8, qSsc-10, qSsc-11, qSsc-12 were detected under two environments, and qSsc-5, qSsc-6, qSsc-7, qSsc-9, qSsc-13, qSsc-14 were only detected under one environment. Mark GDAAS 0603 was detected simultaneously on the linkage groups of both parents and two environments. Those QTLs for starch content could be used in molecular marker-assisted selection of sweetpotato. 【Conclusion】Two genetic linkage map were constructed based on EST-SSR markers, and 17 QTLs for starch content of sweetpotato were identified. Results of the study has enriched the types of molecular markers used for genetic linkage map construction in sweetpotato.

Key words: sweetpotato, starch content, EST-SSR, genetic linkage map, QTL

TANG Dao-bin, ZHANG Kai, Lü Chang-wen, XIE De-bin, FU Ti-hua, WANG Ji-chun. Genetic Linkage Map Construction Based on EST-SSR and Analysis of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) lam.)[J].Scientia Agricultura Sinica, 2016, 49(23): 4488-4506.

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Genetic Linkage Map Construction Based on EST-SSR and Analysis of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) lam.)

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