Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (3): 586-593.doi: 10.3864/j.issn.0578-1752.2014.03.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

A-FABP and MSTN Gene Expression Patterns and Their Correlation in Different Tissues of Goose During Forced-feeding Period

 WANG  Bao-Wei, LIU  Teng, GE  Wen-Hua, ZHANG  Ming-Ai, LI  Zhen, YUE  Bin, CHENG  Fan-Sheng   

  1. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, Shandong
  • Received:2013-04-23 Online:2014-02-01 Published:2013-09-18

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Abstract: 【Objective】Researches on the expression pattern of fat deposition-related genes in geese tissue are meaningful for variety selection. The expression pattern and correlationship between adipocyte fatty acid-binding protein (A-FABP) gene and myostatin (MSTN) gene in different tissues of goose during forced-feeding period were studied. 【Method】 Two hundred Qing Nong Gray geese at the age of 10 weeks were forced-fed and liver, lung, spleen, kidney, chest, leg muscle and abdominal fat during different forced-feeding periods were sampled and total RNA were extracted. The A-FABP and MSTN gene expression pattern in liver, spleen, lung, kidney, chest muscle, leg muscle and abdominal fat of geese were also evaluated using Real Time PCR. Diethyl ether extraction and gas chromatographic methods were employed to detect the content of fat and fatty acids in different tissues. Moreover, the above correlation was evaluated. 【Result】 A-FABP and MSTN genes were expressed in all tissues. A-FABP gene expression in the liver, abdominal fat, leg and chest muscles showed an up-down trend during forced-feeding period with a maximum level (15.90±0.04, 12.72±0.12, 8.88±0.06 and 6.33±0.13) at 24, 12, 18 and 24 d, respectively. MSTN gene expression pattern in the leg muscle and chest muscle showed a similar trend, while no significant difference were observed in the liver, abdominal fat, lungs and kidney (P＜0.01). A-FABP gene expression was closely related to liver weight, liver fat, total polyunsaturated fatty acids and total saturated fatty acids in goose liver. The correlation index were 0.576, 0.510, 0.463 and 0.501, respectively, with significant differences (P＜0.01), while MSTN gene expression exhibited no significant coefficients (P＞0.05). 【Conclusion】 During forced-feeding for 18-24 d, liver weight and the content of fat, total unsaturated fatty acids and total saturated fatty acids in the liver raised significantly. A-FABP and MSTN gene in different goose tissues showed different expression patterns and negative correlations were observed during forced-feeding period. A-FABP gene in goose liver plays a leading role in regulating foie gras traits.

Key words: goose , forced-feeding , A-FABP , myostatin , gene expression , correlation

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