Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (3): 528-536.doi: 10.3864/j.issn.0578-1752.2014.03.012

• HORTICULTURE • Previous Articles     Next Articles

QTL Mapping and Analysis of the Important Agronomic Traits of Beijingjietou×Xishuangbanna Cucumber Recombinant Inbred Lines

 MA  Zheng, BAO  Kai-Liang, LI  Lei, QIAN  Chun-Tao, CHEN  Jin-Feng   

  1. College of Horticulture,Nanjing Agricultural University/State Laboratory for Crop Genetics and Germpalsm Enhancement, Nanjing 210095
  • Received:2013-07-17 Online:2014-02-01 Published:2013-11-27

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Abstract: 【Objective】 A genetic map was constructed using Xishuangbanna cucumber (Cucumis sativus L．var. xishuangbannanesis Qi et Yuan) recombinant lines and QTLs associated with yield, chlorophyll content, fruit related traits, the lateral branch number and the first lateral branch node were identified. These results will be beneficial and helpful to cucumber plant selection and high yield breeding.【Method】Using JoinMap 4.0 software, a genetic linkage map based on 995 SSR primers data from 124 F9 recombinant inbred lines, derived from Beijingjietou cucumber and Xishuangbanna cucumber by using single seed descend method, was constructed. Using WinQTLcart2.5 software, QTLs associated with 12 cucumber traits related to chlorophyll content, fruit lengths, fruit diameters, length of fruit for seed harvest, diameter of fruit for seed harvest, stalk lengths of fruit for seed harvest, and lateral branch, the first lateral branch node were mapped.【Result】The linkage map consisted of 137 SSR (simple sequence repeat) markers, 7 linkage groups that spanned 591.2 cM with an average distance of 4.32 cM. A total of twenty-nine QTLs for the 12 cucumber traits were detected, and 6, 7, 9 and 7 QTLs for chlorophyll content characters, commercial fruit, mature fruit and lateral branch traits were detected, respectively. These QTLs were mapped on chromosomes 1, 2, 3, 4, 6 and 7, respectively. The QTLs explained 5.30%-19.24% of the phenotypic variation. The minimum contribution rate of Ldr4.2 is 5.30%, the maximum contribution rate of Lbn1.2 is 19.24%.【Conclusion】The results of QTL analysis on the 12 cucumber traits will provide a basis for gene fine mapping to unravel the genetic basis of yield related traits in cucumber. Moreover, the obtained genetic linkage map of this RIL population could be used for analysis of other important cucumber traits.

Key words: cucumber , genetic map , agronomic traits , QTL

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