Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (24): 5173-5179.doi: 10.3864/j.issn.0578-1752.2013.24.011

• HORTICULTURE • Previous Articles     Next Articles

Evaluation and QTL Mapping for Resistance to Black Spot (Alternaria brassicicola) in Brassica rapa

 CHEN  Ying-1, 2 , LIU  Li-Wang-1, XIE  Xue-Wen-2, MENG  Lin-2, LIU  Bo-2, WANG  Xiao-Wu-2, LI  Bao-Ju-2, WU  Jian-2   

  1. 1.College of Horticulture, Nanjing Agriculture University, Nanjing 210095;
    2.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081
  • Received:2013-05-13 Online:2013-12-16 Published:2013-08-21

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Abstract: 【Objective】The aim of this study is to evaluate Brassica rapa resistance to black spot disease (Alternaria brassicicola), and map QTL related to this trait. The result will provide theoreticalbasis for genetic improvement on resistance to black spot disease in B. rapa crops.【Method】Conidia of A. brassicicola was sprayed to 30-day-old B. rapa seedlings or detached leaves. Disease index was scored after seedlings or detached leaves being kept in dark and wet for three to six days to determine the number of days for inoculation to distinguish the difference in resistance among B. rapa accessions. Using the optimized method, 70 B. rapa accessions were screened for their resistance to black spot disease. Furthermore, a population with 122 recombinant inbred lines (RILs) was used to map QTL for black spot resistance in B. rapa. 【Result】 It was found that inoculation of detached leaves for five days could well distinguish the resistance to black spot disease in B. rapa. Fourteen from 70 B. rapa accessions were identified as resistant to black spot disease. Moreover, we mapped two QTLs related to resistance to black spot in B. rapa, one of which was identified in both two experiments. 【Conclusion】The study showed that different levels of resistance to black spot disease were clearly distinguished when the detached 3rd/4th true leaves of B. rapa being inoculated with A. brassicicola conidia in dark and wet for 5 days. No accession with high resistance to black spot disease was identified in this study. Two QTLs related to resistance to black spot were mapped on chromosomes A05 and A06, respectively.

Key words: Brassica rapa , black spot , identification for resistance , detached leaf , mapping QTL

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