Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 4996-5002.doi: 10.3864/j.issn.0578-1752.2013.23.016

• HORTICULTURE • Previous Articles     Next Articles

A Preliminary Study on Intergeneric Cross-Incompatibility Between Pistacia vera L. and Pistacia chinensis Bunge

 LI  Xu-Xin-12, CHANG  Yue-Xia-1, QU  Ping-3, GUO  Zhi-Tao-4, CHEN  Fang-4, BAI  Zhi-Ying-3, LU  Bing-She-45   

  1. 1.College of Agronomy, Inner Mongolia University for the Nationalities, Tongliao 028000, Inner Mongolia
    2.College of Forestry, Agricultural University of Hebei, Baoding 071001, Hebei
    3.College of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei
    4.College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding 071001, Hebei
    5.Key Lab of Genetic Resource of Forest and Forest Protection of Hebei Province, Baoding 071001, Hebei
  • Received:2013-05-28 Online:2013-12-01 Published:2013-08-27

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Abstract: 【Objective】 The objective of this experiment is to investigate the fertilization barriers of the intergeneric cross between P. vera and P. chinensis. The pollen morphology, the pollen viability and the progress of cross-pollination and fertilization were observed and the intergeneric cross compatibility between P. vera and P. chinensis was studied. 【Method】 The pollen morphology of male parents was observed by scanning electron microscopy. The pollen germination and the viability were tested by means of in vitro cultivation and the progress of pollen tube growth was observed by fluorescence microscopy. 【Result】There are two and four apertures in pollen surface of P. vera and P. chinensis, respectively, and more dense thorn-like particle in P. chinensis pollen grain than in P. vera. The length of polar axis and equatorial axis of P. chinensis pollen grain were 53.84 μm and 44.85 μm, respectively, which is slightly longer than P. vera pollen grain. The pollen germination rate of P. chinensis Bunge and T-491 were 77.30% and 84.38% respectively, and the pollen viability of P. chinensis Bunge and T-491 were 82.4% and 89.14%, respectively. The pollen of P. chinensis begin to germinate at 4 h, the pollen tubes reached ovary at 48 h and enter embryo sac at 72 h in interspecific-cross after pollination, while the pollen of P. vera T-491 begin to germinate at 2 h, the pollen tubes reached the base of stigma at 12 h and reach to ovary at 24 h. The incompatibility characters such as the pollen tubes bending, swollen and lots of irregular callose depositing in stigma and style were observed in interspecific cross. The percentage of final fruit set was 57% in the self-pollination, while the value was 26% in interspecific-cross which is lower than the former.【Conclusion】A few pollen tubes of P. chinensis could enter the style of P. vera and reached to the ovary. There were some pollen tube elongation growth barriers at interspecific-cross between P. vera and P. chinensis. Abnormal pollen tube growth after pollination may be one of the reasons led to low fruiting rate in intergeneric cross between P. vera and P. chinensis.

Key words: Pistacia vera L. , Pistacia chinensis Bunge , interspecific-cross , pollen , pollen tube

[1]路丙社, 刘忠华, 董源. 阿月浑子引种研究. 北京: 中国林业出版社, 2006: 5.

Lu B S, Liu Z H, Dong Y. Pistachio Introduction Research. Beijing: China Forestry Press, 2006: 5. (in Chinese)

[2]李晓, 张绍铃, 陶书田, 吴华清, 吴俊. 中国樱桃与甜樱桃花粉原位萌发及花粉管生长的差异. 西北植物学报, 2007, 27(3): 429-434.

Li X, Zhang S L, Tao S T, Wu H Q, Wu J. The differences of pollen germination and pollen tube growth between Chinese cherry and sweet cherry. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(3): 429-434. ( in Chinese)

[3]安和祥, Ferguson A R, Bank R J. 猕猴桃种内种间传粉后花粉在雌蕊上行为的观察. 植物引种驯化集刊, 1993, 8: 207-221.

An H X, Ferguson A R, BankR J. Observation on the behaviorof the pollen tube on pistils after introspecific and interspecific pollination of Actindia. Plant Introduction and Acclimatization, 1993, 8: 207-221. ( in Chinese)

[4]王尧, 王玖瑞, 刘孟军, 刘平. 枣树不同杂交组合花粉萌发情况的荧光显微观察. 华北农学报, 2008, 23(增刊): 46-49.

Wang Y, Wang J R, Liu M J, Liu P. Fluorescent microscope observation on pollen germination in different cross combinations of Chinese Jujube. Acta Agriculturae Boreali-Sinica, 2008, 23(Suppl.): 46-49. (in Chinese)

[5]马瑞娟, 杜平, 胡金良, 许建兰, 俞明亮. 桃、李种间远缘杂交亲和性研究. 果树学报, 2005, 22(3): 283-285.

Ma R J, Du P, Hu J L, Xu J L, Yu M L. Studies on the hybridization compatibility between peach and plum. Journal of Fruit Science, 2005, 22(3): 283-285. (in Chinese)

[6]李锋, 张凤芬, 曹希俊, 张冰玉. 李、杏及杂种间远缘杂交亲和性研究. 吉林农业大学学报, 1995, 17(4): 36-39.

Li F, Zhang F F, Cao X J, Zhang B Y. Study on distant hybridization and affinity of the hybrid between the plum and apricot. Journal of Jilin Agricultural University, 1995, 17(4): 36-39. (in Chinese)

[7]袁琳, 克热木•伊力, 张利权. NaCl胁迫对阿月浑子实生苗活性氧代谢与细胞膜稳定性的影响. 植物生态学报, 2005, 29(6): 985-991.

Yuan L, Karim A L, Zhang L Q. Effects of NaCl stress on      active oxygen metabolism and membrane stability in Pistacia vera seedlings. Acta Phytoecologica Sinica, 2005, 29(6): 985-991. (in Chinese)

[8]周如久, 倪志云, 路丙社, 白志英, 李献明. 阿月浑子花序生长和花期物候学研究. 西北植物学报, 2006, 26(8): 1579-1583.

Zhou R J, Ni Z Y, Lu B S, Bai Z Y, Li X M. Inflorescence growth and flowering phenology of pistachio. Acta Botanica Boreal-Occidentalia Sinica, 2006, 26(8): 1579-1583. (in Chinese)

[9]李旭新, 白志英, 路丙社, 庞曼, 王洁, 冯献宾, 董倩. 阿月浑子大、小孢子的发生及雌、雄配子体发育. 园艺学报, 2011, 38(8): 1447-1454.

Li X X, Bai Z Y, Lu B S, Pang M, Wang J, Feng X B, Dong Q. Megasporogenesis, microsporogenesis and development of gametophytes of Pistacia vera L.. Acta Horticulturae Sinica, 2011, 38(8): 1447-1454.(in Chinese)

[10]王超, 郝爱丽, 白志英, 路丙社. 13个阿月浑子品种的RAPD分析. 园艺学报, 2010, 37(3): 475-478.

Wang C, Hao A L, Bai Z Y, Lu B S. RAPD analysis of 13 pistachio (Pistacia vera L.) cultivars. Acta Horticulturae Sinica, 2010, 37(3): 475-478. (in Chinese)

[11]Parfitt D E, Badenes M L, Ferguson L, Maranto J, Almehdi A, Guo Y L. Pistachio breeding and genetics program at the university of California. Acta Horticulturae, 1995, 419: 279-280.

[12]Tajabadipour A, Ghadarijani M, Zadehparizi R. Closed pollination for preparing interspecific hybridization of pistacia genus and determination of a hybrid resistant to gummosis. Acta Horticulturae, 2006, 726: 637-642.

[13]Uzun M, Acar I, Atli H S, Arpaci S, Sarpkaya K and Gözel H. Breeding of new pistachio cultivars by hybridization in Turkey. Acta Horticulturae, 2011, 912: 321-325.

[14]Atli H S, Kaska N. Pistachio rootstocks breeding by crossing Pistacia Vera L. and Pistacia Khinjuk stocks. Acta Horticulturae, 2002, 591: 83-89.

[15]Gouta H A, Chelli C, Ghrab M, Ben A I, Mimoun M B, Hellali R. Preliminary results on the effects of different pistacia species pollen on fruit characteristics of two commercial cultivars ‘mateur’ and ‘El guettar’. Acta Horticulturae, 2002, 591: 405-409.

[16]Ak B E. Effects of some Pistacia spp. pollen on different parts of the pistachio fruits such as hull, shell and kernel//Zakynthinos G. XIV GREMPA Meeting on Pistachios and Almonds. Zaragoza: CIHEAM, 2010: 129-133.

[17]Behboodi B S, Ghaffari M. Pollen morphology and analysis of Iranian wild pistachio//Oliveira M M, Cordeiro V. XIII GREMPA Meeting on Almonds and Pistachios. Zaragoza: CIHEAM, 2005: 123-127.

[18]Dicenta F, Martínez-García P J, Ortega E. Influence of type of pollen-pistil interaction in pollen tube growth and fruit set//Zakynthinos G. XIV GREMPA Meeting on Pistachios and Almonds. Zaragoza: CIHEAM, 2010: 147-151.

[19]Ghrab M，Ben Mimoun M，Triki H, Gouta H. Evaluation of the performance of seventeen male pistachio-tree specimens. Acta Horticulturae, 2002, 591: 473-477.

[20]孟金陵, 刘定富, 蔡得田.植物生殖遗传学. 北京: 科学出版社, 1997: 300-305.

Meng J L, Liu D F, Cai D T. Plant Reproductive Genetics. Beijing: Science Press, 1997: 300-305. (in Chinese)

[21]Hopping M E. Floral biology, pollination, and fruit set//Warrington I J, Weston G C. Kiwifruit: Science and Management. Auckland: Ray Richards Press, 1990: 71-96.

[22]李辛雷, 陈发棣, 赵宏波. 菊属植物远缘杂交亲和性研究. 园艺学报, 2008, 35(2): 257-262.

Li X L, Chen F D, Zhao H B. Compatibility of interspecific cross in Dendranthema genus. Acta Horticulturae Sinica, 2008, 35(2): 257-262. (in Chinese)

[23]睢薇, 丁晓东, 霍俊伟, 迟慧梅. 草原樱桃与欧洲甜樱桃远缘杂交不亲和原因初探. 东北农业大学学报, 1999, 30(2): 148-153.

Sui W, Ding X D, Huo J W, Chi H M. Preliminry studies on distant cross-incompatibility between Cerasus Fruticosa Pall and Caviuml. Journal of Northeast Agricultural University, 1999, 30(2): 148-153. (in Chinese)

[24]贾爱平, 王飞, 张潮红, 姚春潮, 刘旭峰. 中华猕猴桃品种间亲和性研究. 园艺学报, 2010, 37(11): 1829-1835.

Jia A P, Wang F, Zhang C H, Yao C C, Liu X F. Cross compatibility among different cultivars of actinidia chinensis planch. Acta Horticulturae Sinica, 2010, 37(11): 1829-1835. (in Chinese)
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