Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 4996-5002.doi: 10.3864/j.issn.0578-1752.2013.23.016

• HORTICULTURE • Previous Articles     Next Articles

A Preliminary Study on Intergeneric Cross-Incompatibility Between Pistacia vera L. and Pistacia chinensis Bunge

 LI  Xu-Xin-12, CHANG  Yue-Xia-1, QU  Ping-3, GUO  Zhi-Tao-4, CHEN  Fang-4, BAI  Zhi-Ying-3, LU  Bing-She-45   

  1. 1.College of Agronomy, Inner Mongolia University for the Nationalities, Tongliao 028000, Inner Mongolia
    2.College of Forestry, Agricultural University of Hebei, Baoding 071001, Hebei
    3.College of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei
    4.College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding 071001, Hebei
    5.Key Lab of Genetic Resource of Forest and Forest Protection of Hebei Province, Baoding 071001, Hebei
  • Received:2013-05-28 Online:2013-12-01 Published:2013-08-27

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Abstract: 【Objective】 The objective of this experiment is to investigate the fertilization barriers of the intergeneric cross between P. vera and P. chinensis. The pollen morphology, the pollen viability and the progress of cross-pollination and fertilization were observed and the intergeneric cross compatibility between P. vera and P. chinensis was studied. 【Method】 The pollen morphology of male parents was observed by scanning electron microscopy. The pollen germination and the viability were tested by means of in vitro cultivation and the progress of pollen tube growth was observed by fluorescence microscopy. 【Result】There are two and four apertures in pollen surface of P. vera and P. chinensis, respectively, and more dense thorn-like particle in P. chinensis pollen grain than in P. vera. The length of polar axis and equatorial axis of P. chinensis pollen grain were 53.84 μm and 44.85 μm, respectively, which is slightly longer than P. vera pollen grain. The pollen germination rate of P. chinensis Bunge and T-491 were 77.30% and 84.38% respectively, and the pollen viability of P. chinensis Bunge and T-491 were 82.4% and 89.14%, respectively. The pollen of P. chinensis begin to germinate at 4 h, the pollen tubes reached ovary at 48 h and enter embryo sac at 72 h in interspecific-cross after pollination, while the pollen of P. vera T-491 begin to germinate at 2 h, the pollen tubes reached the base of stigma at 12 h and reach to ovary at 24 h. The incompatibility characters such as the pollen tubes bending, swollen and lots of irregular callose depositing in stigma and style were observed in interspecific cross. The percentage of final fruit set was 57% in the self-pollination, while the value was 26% in interspecific-cross which is lower than the former.【Conclusion】A few pollen tubes of P. chinensis could enter the style of P. vera and reached to the ovary. There were some pollen tube elongation growth barriers at interspecific-cross between P. vera and P. chinensis. Abnormal pollen tube growth after pollination may be one of the reasons led to low fruiting rate in intergeneric cross between P. vera and P. chinensis.

Key words: Pistacia vera L. , Pistacia chinensis Bunge , interspecific-cross , pollen , pollen tube

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