Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4328-4335.doi: 10.3864/j.issn.0578-1752.2013.20.016

• HORTICULTURE • Previous Articles     Next Articles

Interspecific Hybridization Between Chrysanthemum morifolium ‘Nannongyinshan’ and C. zawadskii and Identification of Waterlogging Tolerance of Their Hybrid

 WANG  Liang, WANG  Chu-Chu, JIANG  Jia-Fu, CHEN  Su-Mei, FANG  Wei-Min, TENG  Nian-Jun, GUAN  Zhi-Yong, LIAO  Yuan, CHEN  Fa-Di   

  1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095
  • Received:2013-04-03 Online:2013-10-15 Published:2013-06-03

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Abstract: 【Objective】To improve the waterlogging tolerance of spray cut chrysanthemum, an interspecific hybridization was combined between Chrysanthemum morifolium ‘Nannongyinshan’ and C. zawadskii. The phenotypic characteristics and waterlogging tolerance of the parents and the hybrid were identified. 【Method】Interspecific hybridization was made between the female parent C. morifolium ‘Nannongyinshan’, which is susceptible to waterlogging ,and the male parent C. zawadskii, which is tolerant. The hybridity was identified by morphological traits and SRAP molecular markers. Waterlogging tolerance was investigated by changes in leaf relative water content (RWC), contents of chlorophyll (Chl) and malondialdehyde (MDA), and activities of antioxidant enzymes. 【Result】 Only one putative hybrid was obtained through the interspecific hybridization. Morphological characteristics including the plant height, leaf length, leaf width, number of tubular florets and ray florets of the hybrid were different with that of the parents. In the hybrid, both the male and the female specific bands were observed by SRAP molecular markers analysis. Thus the progeny was a real interspecific hybrid. The decrease of RWC and chl and the increase of MDA of the hybrid were significantly lower than that of maternal ‘Nannongyinshan’, while the activities of antioxidant enzymes were higher. 【Conclusion】 The results indicated that the hybrid was more tolerant to waterlogging than ‘Nannongyinshan’. Therefore, it is possible to obtain new waterlogging tolerant cut chrysanthemum germplasm via interspecific hybridization to introduce the tolerant trait of C. zawadskii.

Key words: Chrysanthemum morifolium , C. zawadskii , interspecific hybridization , waterlogging tolerance

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