Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (18): 3822-3829.doi: 10.3864/j.issn.0578-1752.2011.18.013

• HORTICULTURE • Previous Articles     Next Articles

Study on Cross-Compatibility of Interspecific Hybridization and Selfing in Clivia Lindl.

WANG  Chong, LEI  Jia-Jun, JIANG  Chuang, XING  Gui-Mei   

  1. 1.沈阳农业大学园艺学院
    2.辽宁省农业科学院现代园艺展示中心
    3. 辽宁省农业科学院花卉研究所
  • Received:2011-02-22 Revised:2011-05-16 Online:2011-09-15 Published:2011-06-20

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Abstract: 【Objective】 Three Clivia species or varieties, including C. miniata, C. miniata var. citrina and C. nobilis, were used as experimental materials in this research, and the interspecific reciprocal crosses and selfing were carried out in order to study the compatibility. 【Method】 The pollen viability of the species or varieties was determined via culture in vitro，and the pollen germination and pollen tube behavior after pollination were observed via fluorescence microscope. 【Result】 The results indicated that the pollen viability of C. nobilis was the lowest of 42.6%, whereas it was above 80% in both C. miniata and C. miniata var. citrina. The fruit setting rate in the cross of C. nobilis × C. miniata and C. nobilis × C. miniata var. citrina was 15.9% and 19.1% respectively, and the average number of seeds per fruit was 1.2 and 1.4, respectively, but the fruit setting rate and the seeds per fruit were higher in their inverse cross combinations. The fruit setting rate of reciprocal crosses between C. miniata and C. miniata var. citrina was above 50%, and the seeds per fruit reached above 5, which showed the compatibility was good. In contrast to selfing of C. nobilis, the pollens of C. nobilis in interspecific crosses germinated later and elongated slower obviously, and few pollen tubes reached ovary within 96 h after pollination via fluorescence microscope. The calloses observed on stigmas of C. nobilis hindered pollen germination when C. nobilis was used as the female parent. 【Conclusion】 The cross-compatibility was different in interspecific reciprocal crosses in Clivia. The fruit setting rate was higher while C. nobilis was used as the male parent. The fruit setting rate and the average number of seeds per fruit in interspecific reciprocal crosses were obviously lower than in selfing except the reciprocal cross combination of C. miniata and C. miniata var. citrina, in which there was a relative high value. The calloses were observed in pollen tubes and on stigma in all interspecific cross combinations.

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CLC Number: 

  • S 685.99

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