Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (18): 3887-3893.doi: 10.3864/j.issn.0578-1752.2013.18.019

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

The Expression of Paternal Imprinting Genes in Bovine Early Stage Embryo

 ZHANG  Chun-Qiang, ZHAO  De-Chao, HAN  Zhi-Ling, ZHANG  Wen-Guang, ZHANG  Jia-Xin   

  1. The Key Lab of Animal Genetics, Breeding, and Reproduction/The College of Animal Science of Inner Mongolia Agricultural University, Hohhot 010018
  • Received:2013-05-02 Online:2013-09-15 Published:2013-05-21

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Abstract: 【Objective】The objective of the experiment is to investigate the expression pattern of paternal imprinting genes in bovine early stage embryo.【Method】The expression profile of paternal imprinting genes Gnas, Grb10, and Xist were detected in bovine embryo derived from in vitro fertilization (IVF) and parthenogenetic activation (PA) by real-time PCR. 【Result】 There were no difference in imprinted expression profile of Gnas between IVF and PA embryo. Grb10 expression was significantly higher during 2-cell stage in PA embryo than that of IVF embryo, however, no difference was observed in expression of Grb10 between IVF and PA embryos from 4-cell satge to blastcyst stage. Xist expression was similar between PA and IVF embryo from 2-cell to 8-cell stage. But in the later stage, Xist expression was higher significantly in PA embryo than that in IVF embryo. 【Conclusion】Gnas, Grb10 and Xist were expressed differently in bovine IVF embryo and PA embryo from 2-cell stage to the blastocyst stage, and they might play an important role in bovine embryo development.

Key words: bovine , paternal imprinted genes , early stage embryo , expression

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