Abstract: 【Objective】The objective of this study is to identify the most tolerant stage of azuki bean beetle, Callosobruchus chinensis L. (Coleoptera: Bruchidae) to low and high temperatures, and obtain different temperature-exposure time to kill the various stages of the insect in storage. And to provide a theoretic foundation for popularizing and applying low and high temperatures for the control of C. chinensis.【Method】Four development stages were exposed for different times at -5 and 40℃ to get the most tolerant stage of C. chinensis for low and high temperatures. Based on the above experimental results, the most cold-tolerant stage was used for consequent bioassays at -5, -10 and - 20℃, and the heat-tolerant stage at 40, 45 and 50℃. 【Result】 Pupa and larva were more cold tolerant stages, LT50 at -5℃ for eggs, larvae, pupae and adults were 12.57, 24.93, 30.54 and 15.76 h, respectively. Pupa was relatively more heat tolerant than other three stages, the LT50 at 40℃ for eggs, larvae, pupae and adults were 4.29, 17.76, 22.33 and 14.50 h, respectively. The LT50 for pupae were 30.54, 6.50 and 0.96 h at -5, -10 and -20℃, respectively. The LT99 were 189.70, 33.81 and 2.90 h, respectively. The LT50 of pupae at 40, 45, and 50℃ were 22.33, 3.64 and 0.85 h, respectively. The LT99 were 169.43, 17.77 and 3.71 h, respectively. 【Conclusion】Both cold tolerance and heat tolerance of pupa are higher than other three life stages, and different temperature-exposure times to kill the pupa stage of the insect are obtained. It is feasible to utilize low and high temperatures to control C. chinensis in storage.

Key words: grain storage , Callosobruchus chinensis , pest management , developmental stages , temperature