抗真菌转基因水稻对稻田靶标病害和非靶标病虫害的影响

Abstract

Abstract: 【Objective】The study aimed at the effects of transgenic rice with four anti-fungal genes on the target fungal diseases and the non-target bacterial diseases and insect pests in paddy fields. 【Method】 Two transgenic rice lines, E122-1 and E127-1, which were overexpression of rice acidic chitinase gene (RAC22) and rice basic chitinase gene (RCH10) and transformed with alfalfa β-1,3- glucanase gene (β-Glu) and barley ribosome inactivating protein gene (B-RIP), were tested for their effects on the target fungal diseases (rice blast, rice false smut and rice brown spot) and the non-target bacterial diseases (rice bacterial blight and rice bacterial leaf streak and insect pests (rice yellow stem borer and rice leaf roller) by means of innoculation in greenhouse and investigation in paddy fields, compared with their untransformed counterpart E32. 【Result】Both E122-1 and E127-1 showed broad spectrum of resistance against rice blast isolates and were significantly lower in the percentages of infected plants and the disease index resulted from rice false smut or rice brown spot, but had no significant difference in the percentages of susceptible plants and the disease index resulted from rice bacterial blight or rice bacterial leaf streak, the percentages of “dead heart” plants resulted from rice yellow stem borer and the percentages of leaf-rolled plants resulted from rice leaf roller in comparison with the counterpart E32. On the plants with resistance to rice bacterial blight, both E122-1 and E127-1 had significant higher number plants with no infected symptom and had significant lower number plants with the 3rd scale infected symptom than that of the counterpart E32. 【Conclusion】 Both E122-1 and E127-1 exhibited a high resistance to rice blast, rice false smut and rice brown spot, and did not cause obvious population increase of rice bacterial blight, rice bacterial leaf streak, rice yellow stem borer and rice leaf roller as compared to their counterpart during growing period in paddy fields.

Key words: transgenic rice , anti-fungal gene , non-target organism , disease , insect pest

LI Wei, GUO Jian-Fu, YUAN Hong-Xu, LI Yue-Ren, LIU Yue-Lian, HUANG Yong-Xiang, JIANG Shi-He. Effects of Anti-Fungal Transgenic Rice on the Target Fungal Diseases and the Non-Target Bacterial Diseases and Insect Pests in Paddy Fields[J].Scientia Agricultura Sinica, 2012, 45(24): 5032-5039.

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