Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (23): 4836-4843.doi: 10.3864/j.issn.0578-1752.2012.23.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Fe and Al Oxides on Adsorption of Bt Toxin by Several Soils in South of China

 FU  Qing-Ling, DENG  Ya-Li, HU  Hong-Qing, WEI  Xia, HAN  Xiao-Fang, LI  Yan   

  1. 1.College of Resource and Environment, Huazhong Agricultural University, Wuhan 430070;
    2. Tianjin Institute of Agricultural Resource and Environmental Sciences, Tianjin 300192
  • Received:2012-04-18 Online:2012-12-01 Published:2012-06-19

Abstract: 【Objective】This paper studied the interaction between Bt toxin and several soils in South of China (Latosols, Latosolic red soil, red soil, yellow soil and paddy soil), and explored the effects of Fe and Al oxides on the adsorption. 【Method】 The adsorption characteristics of Bt toxin by different soils were measured by isothermal adsorption method and fitted by Langmuir equation. 【Result】 The results indicated that except the isotherm curve of Bt toxin adsorbed by Latosols and red soil after removal of free Fe and Al oxides were H type, the adsorption isotherms of Bt toxin by other tested soils were all L-shaped and fitted to Langmuir equation. The adsorption amount of Bt toxin by soils after removal organic bound Fe and Al oxides increased compared to their original soils, while the adsorption amount by soils after removal other species of Fe and Al oxides decreased, and the greatest impact was observed after the removal of free Fe and Al oxides. The adsorption affinity (KL) of Bt toxin adsorbed by soils after removal of organic bound Fe and Al oxides was the highest, and the lowest was shown on soils after removal of free Fe and Al oxides.【Conclusion】 Except organic Fe and Al oxides, Fe and Al oxides (such as the free, amorphous Fe and Al oxides, etc.) promoted the adsorption of Bt toxin by soils, which could provide a reference for the assessment of the environmental risk of Bt protein in soil.

Key words: Bt toxin , adsorption , soil , Fe and Al oxides

[1]James C. Global status of commercialized biotech/GM crops: 2009. ISAAA Brief No. 41. New York: International Service for the Acquisition of Agri-Biotech Applications (ISAAA), 2009.

[2]Stotzky G. Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis and of bacterial DNA bound on clays and humic acids. Journal of Environmental Quality, 2000, 29: 691-705.

[3]Stotzky G. Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants. Plant and Soil, 2004, 266: 77-89.

[4]Crecchio C, Stotzky G. Biodegradation and insecticidal activity of the toxin from Bacillus thuringiensis subsp. kurstaki bound on complexes of montmorillonite-humic acids-Al hydroxypolymers. Soil Biology and Biochemistry, 2001, 33: 573-581.

[5]孙彩霞, 陈利军, 武志杰. 转Bt基因作物毒素土壤存活特性及与土壤性质的关系研究进展. 应用生态学报, 2002, 13(11): 1478-1482.

Sun C X, Chen L J, Wu Z J. Research advances in soil persistence characteristics of toxins released by transgenic Bt crops and their relationships with soil properties. Chinese Journal of Applied Ecology, 2002, 13(11): 1478-1482. (in Chinese)

[6]李云河, 王桂荣, 吴孔明, 张永军, 原国辉, 郭予元. Bt作物杀虫蛋白在农田土壤中残留动态的研究进展. 应用与环境生物学报, 2005, 11(4): 504-508.

Li Y H, Wang G R, Wu K M, Zhang Y J, Yuan G H, GuoY Y. Progress in persistence dynamics of insecticidal proteins released from Bt crops in cropland. Chinese Journal of Applied and Environmental Biology, 2005, 11(4): 504-508. (in Chinese)

[7]王洪兴, 陈  欣, 唐建军, 志水胜好. 释放后的转抗病虫基因作物对土壤生物群落的影响. 生物多样性, 2002,10(2): 232-237.

Wang H X, Chen X, Tang J J, Shimizu katsuyoshi. Influence of released transgenic pest- and disease-resistant crops on plant-associated microorganisms in soil. Biodiversity Science, 2002, 10(2): 232-237. (in Chinese)

[8]Zhou X Y, Huang Q Y, Chen S W, Yu Z N. Adsorption of the insecticidal protein of Bacillus thuringiensis on montmorillonite, kaolinite, silica, goethite and red soil. Applied Clay Science, 2005, 30: 87-93.

[9]Pagel-Wieder S, Niemeyer J, Fischer W R, Gessler F. Effects of physical and chemical protection of soils on adsorption of the insecticidal protein (Cry1Ab) from Bacillus thuringienses at Cry1Ab protein concentrations relevant for experimental field sites. Soil Biology and Biochemistry, 2007, 39: 3034-3042.

[10]Fu Q L, Peng Y W, Huang T, Hu H Q, Deng Y L, Yu X. Effects of ionic strength and sesquioxides on adsorption of the toxin of Bacillus thuringiensis subsp. kurstaki on soils. Pedosphere, 2012, 22(1): 96-102.

[11]Fu Q L, Hu H Q, Chen S W, Huang Q Y, Liang W. Adsorption of the insecticidal protein of Bacillus thuringiensis subsp. kurstaki by some Chinese soils: effects of organic acid ligands addition. Plant and Soil, 2007, 296: 35-41.

[12]Fu Q L, Wang W Q, Hu H Q, Chen S W. Adsorption of the insecticidal protein of Bacillus thuringiensis subsp. kurstaki by minerals: effects of inorganic salts. European Journal of Soil Science, 2008, 59: 216-221.

[13]姚艳玲, 崔海瑞, 卢美贞, 忻 雅. 转基因植物释放Bt毒素的土壤环境行为与生物学效应. 土壤学报, 2005, 42(6): 1024-1029.

Yao Y L, Cui H R, Lu M Z, Xin Y. Environmental behavior and biological effects of Bt toxins released from Bt-transgenic plants in soil. Acta Pedologica Sinica, 2005, 42(6): 1024-1029. (in Chinese)

[14]白耀宇, 蒋明星, 程家安, 姜永厚. 转Bt 基因作物Bt 毒蛋白在土壤中的安全性研究. 应用生态学报, 2003, 14(11): 2062-2066.

Bai Y Y, Jiang M X, Cheng J A, Jiang Y Y. Advances in safety studies of soil Bt toxin proteins released from transgenic Bt crops. Chinese Journal of Applied Ecology, 2003, 14(11): 2062- 2066. (in Chinese)

[15]Quiquampoix H. Mechanisms of protein adsorption on surfaces and consequences for extracellular enzyme activity in soil. Soil Biochemistry, 2000, 10: 171-206.

[16]熊  毅.土壤胶体. 第二册.北京: 科学出版社, 1985.

Xiong Y. Research Methods of Soil Colloid. Soil Colloids. Vol.2. Beijing: Science Press, 1985. (in Chinese)

[17]Langmuir I. The adsorption of gases on plane surfaces of glass, mica and platinum. Journal of the American Chemical Society, 1918, 40: 1361-1403.

[18]Sepelyak R J, Feldkamp J R, Moody T E, White J L, Hem S L. Adsorption of pepsin by aluminum hydroxide I: Adsorption mechanism. Journal of Pharmaceutical Science, 1984, 73(11): 1514-1517.

[19]Huang Q Y, Jiang M H, Li X Y. Effects of iron and aluminum oxides and kaolinite on adsorption and activity of invertase. Pedosphere, 1998, 8(3): 251-260.

[20]Madliger M, Sander M, Schwarzenbach R P. Adsorption of transgenic insecticidal Cry1Ab protein to SiO2. 2. patch-controlled electrostatic attraction. Environmental Science and Technology, 2010, 44: 8877-8883.

[21]Madliger M, Gasser C A, Schwarzenbach R P, Sander M. Adsorption of transgenic insecticidal Cry1Ab protein to silica particles effects on transport and bioactivity. Environmental Science and Technology, 2011, 45: 4377-4384.

[22]Tapp H, Calamai L, Stotzky G. Adsorption and binding of the insecticidal proteins from Bacillus thuringicnsis subsp. kurstaki and subsp. tecnebrionis on clay minerals. Soil Biology and Biochemistry, 1994, 26: 663-679.

[23]Muchaonyerwa P, Chevallier T, Pantani O L, Nyamugafata P, Mpepereki S, Chenu C. Adsorption of the pesticidal toxin from Bacillus thuringiensis subsp. tenebrionis on tropical soils and their particle-size fractions. Geoderma, 2006, 133: 244-257.

[24]Malekani K, Rice J A, Lin J S. The effect of sequential removal of organic matter on the surface morphology of humin. Soil Science, 1997, 162: 333-342.

[25]刘冬碧, 贺纪正, 刘  凡, 李学垣. 中南地区几种土壤的表面电荷特性Ⅳ.氧化铁铝对土壤表面电荷性质的影响. 土壤学报, 2001, 38(1): 123-127.

Liu D B, He J Z, Liu F, Li X Y. Surface charge characteristics of soils in central and southern China IV. The effect of Fe and Al oxides on surface charge characteristics of soils. Acta Pedologica Sinica, 2001, 38(1): 123-127. (in Chinese)
[1] XU YangHaoJun, CHEN LiMing, YANG ShiQi, TANG YiFan, TAN XueMing, ZENG YongJun, PAN XiaoHua, ZENG YanHua. Effects of Long-Term Different Straw Returning Methods on Soil Organic Carbon, Nutrients and Aggregate Formation in Different Soil Layers of Double Cropping Rice Field [J]. Scientia Agricultura Sinica, 2026, 59(7): 1492-1506.
[2] LI YongJuan, ZHANG YueTong, WANG YiBo, ZHAO ChangJiang, SONG Jie, CHEN XueLi, YAO Qin. Effects of Biochar Application on the Abundance and Community Composition of Nitrogen-Fixing Microbial nifH Gene in Soybean Rotation and Continuous Cropping Systems [J]. Scientia Agricultura Sinica, 2026, 59(6): 1272-1285.
[3] WEI YuanHui, YU YiHui, LI ZiJun, DING WenJie, TU WenLong, MAO YanLing. Effects of Long-Term Fertilization on Soil Organic Carbon Structure and Carbon-Fixing Bacterial Community Structure in Yellow-Mud Paddy Soil [J]. Scientia Agricultura Sinica, 2026, 59(5): 1020-1033.
[4] LI WenHu, LI HaiFeng, DU YuPeng, DING YuLan, LUO YiNuo, LI YuKe, SHE WenTing, ZHANG Feng, TENG Yu, ZHANG SiQi, HUANG Cui, LI XiaoHan, LIU JinShan, WANG ZhaoHui. Regional Differences in Wheat Zinc Uptake and Translocation Responses to Soil Zinc Fertilization [J]. Scientia Agricultura Sinica, 2026, 59(5): 1034-1047.
[5] YAN YanGe, ZHANG ShuiQin, XU Meng, XU JiuKai, LI YanTing, ZHAO BingQiang, YUAN Liang. Transformation Characteristics of Dextran-Modified Urea in Fluvo- Aquic Soil [J]. Scientia Agricultura Sinica, 2026, 59(5): 1048-1059.
[6] WEN YuBin, BAI ShanShan, CAI ZeJiang, SUN Nan, XU MingGang. Effects of Organic Materials on Soil Microbial Biomass and Its Acidity Regulation Mechanism [J]. Scientia Agricultura Sinica, 2026, 59(4): 834-849.
[7] GUO FuCheng, TANG HaiJiang, HAO XinYi, MA GuoLin, YANG JiuJu, HUANG LinFeng, TIAN Lei, WANG Bin, LUO ChengKe. Effects of Different Irrigation Methods on Water-Salt Transport, Rice Yield, and Water Use Efficiency in Saline Soil in Ningxia [J]. Scientia Agricultura Sinica, 2026, 59(4): 750-764.
[8] LIU MengYang, LIU Jie, CHEN Xiang, WANG QingYun, LUO LaiChao, QI YongBo, TIAN Da, LI JinCai, CHAI RuShan. Effects of Long-Term Straw Return on Distribution of Aggregates and Phosphorus Fractions in Shajiang Black Soil [J]. Scientia Agricultura Sinica, 2026, 59(3): 575-588.
[9] XIAN QingLin, XIAO JianKe, GAO AQing, GAO LiChuang, LIU Yang. Effects of Planting Patterns Combined with Soil Moisture Measurement and Supplementary Irrigation on the Yield and Water Use Efficiency of Winter Wheat [J]. Scientia Agricultura Sinica, 2026, 59(3): 589-601.
[10] YAN TingLin, DU YaDan, HU XiaoTao, WANG He, LI XiaoYan, WANG YuMing, NIU WenQuan, GU XiaoBo. The Impacts of Nitrogen Fertilizer Organic Alternatives Under Aerated Drip Irrigation on Cotton Yield and Water Use Efficiency Under Deficit Irrigation Conditions [J]. Scientia Agricultura Sinica, 2026, 59(3): 602-618.
[11] CHEN GuiPing, WEI JinGui, GUO Yao, LI Pan, WANG FeiEr, QIU HaiLong, FENG FuXue, YIN Wen. Synergistic Effects of Wide-Narrow Row and Density Enhancement on the Photosynthetic Characteristics and Resource Utilization of Maize in Oasis Irrigation Areas [J]. Scientia Agricultura Sinica, 2026, 59(2): 278-291.
[12] WANG RenZhuo, LI YueYing, HUANG ShaoMin, JIANG GuiYing, ZHANG Qi, LIU ChaoLin, YANG Jin, WANG MengRu, WANG BeiBei, LIU Fang, GUO DouDou, JIE XiaoLei, SONG Lian, LIU ShiLiang. Effects of Long-Term Combination of Organic and Inorganic Fertilizers on Bacterial Community Structure, Ecological Network, and Key Species in Fluvo-Aquic Soil [J]. Scientia Agricultura Sinica, 2026, 59(2): 354-367.
[13] GUO HuiTing, SUN YanWen, NIU YiHeng, LI YaPeng, LI JianHua, XU MingGang. Fertilization Significantly Changed Soil Bacterial Diversity and Dominant Microbial Community in Croplands of Northern China—Meta Analysis [J]. Scientia Agricultura Sinica, 2026, 59(1): 114-128.
[14] LÜ XuDong, SUN ShiYuan, LI YaNan, LIU YuLong, WANG YanQun, FU Xin, ZHANG JiaYing, NING Peng, PENG ZhengPing. Effects of Intelligent Mechanized Layered Fertilization on Root-Soil Nutrient Distribution and Yield in Wheat Fields [J]. Scientia Agricultura Sinica, 2026, 59(1): 129-146.
[15] LU Hao, ZHANG MingLong, HAN Mei, YAN QingBiao, LI ZhengPeng, YIN Wen, FAN ZhiLong, HU FaLong, CHAI Qiang. Green Manure Returning via Sheep Digest with Nitrogen Fertilizer Reduction are Beneficial to Improve Wheat Yield and Soil Quality at Qinghai-Tibet Plateau [J]. Scientia Agricultura Sinica, 2026, 59(1): 147-160.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!