Scientia Agricultura Sinica

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Intraspecific Variability of Tylenchulus semipenetrans Populations on Citrus and Chinese Fir

LIU Guo-kun; CHEN Juan; XIAO Shun; PAN Dong-ming; ZHANG Shao-sheng   

  1. 1、College of Plant Protection, Fujian Agriculture and Forestry University,Fuzhou 350002;
    2、College of Horticulture,Fujian Agriculture and Forestry University, Fuzhou 350002
  • Received:2010-10-13 Online:2011-05-05 Published:2011-02-22

Abstract: 【Objective】 The objective of this study is to examine the variation in morphology and rDNA-ITS region (ITS1- 5.8S-ITS2) of Tylenchulus semipenetrans on citrus or Chinese fir (Cunninghamia lanceolata), and to analyze genetic variation and phylogenetic relationships among populations. 【Method】The ITS regions of 21 populations of T. semipenetrans collected from citrus groves in Fujian, Sichuan, and Guizhou provinces of China and 6 populations of T. semipenetrans collected from Chinese fir plantations in Fujian province were amplified, cloned and sequenced. Sequence alignment and phylogenetic analysis were performed by using related softwares. Morphometric characters were analyzed on 3 populations on citrus and one population on Chinese fir.【Result】 Comparison with populations on citrus and populations on Chinese fir, sequence similarities of ITS1 were 93.2%-99.3%, sequence similarities of ITS2 were 92.4%-100%. Six populations on Chinese fir and some populations on citrus collected from Fujian province gathered at the same main branch of phylogenetic tree based on the sequences of ITS1-5.8S-ITS2 of 27 populations using NJ method. The significant differences in some morphometric characters were found among 4 populations of T. semipenetrans. New morphological variations in the tail of 2nd stage juvenile in population were described. 【Conclusion】The high sequence similarity of ITS region of populations of T. semipenetrans on citrus or Chinese fir were demonstrated. Sequence variations of ITS region couldn’t be distinguished different populations of T. semipenetrans. The populations of T. semipenetrans on Chinese fir may have same biogeographic origin with some populations of T. semipenetrans on citrus in Fujian province. The difference in some morphometric characters and morphological variations in the tail of 2nd stage juvenile among populations may be normal variations.

Key words: citrus , Chinese fir , Tylenchulus semipenetrans , ITS , morphometry , intraspecific variability

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