Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (24): 5083-5091.doi: 10.3864/j.issn.0578-1752.2011.24.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Modulation of Dietry Vitamin E on Economic Characterization of Immunosupressed Laying Hens

 PENG  Bing-Yong, GAO  Yu-Peng, WANG  Chun-Jiang, ZHENG  Hai-Zu, HUANG  Fu-Yi-Fan, JI  Jian-Xin   

  1. 1.西北农林科技大学动物科技学院，陕西杨凌 712100
  • Online:2011-12-15 Published:2011-12-15

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Abstract: 【Objective】The modulation of dietary vitamin E levels on immunosuppressed laying hens induced by cyclophosphamide (CTX) was studied. 【Method】Two hundred and seventy Nick Red laying hens were randomly divided into 5 groups and each group had 3 replications with 18 birds. Laying hens from group Ⅲ, Ⅳ, and Ⅴ(birds treated with vitamin E) were fed diets supplemented with 50, 100 and 200 mg•kg-1 vitamin E, respectively, whereas group Ⅰ and Ⅱ received only the corn-soybean meal basal diets with 44.59 mg•kg-1 of vitamin E. Laying hens in group Ⅱ-Ⅳ(birds challenged with CTX) were injected in the thigh muscle with CTX at 80 body weight for 3 days, and that in groupⅠ(control group) were injected with the same amount of sterile saline. 【Result】Supplemental vitamin E significantly increased egg production, feed efficiency and nutrient digestibility (P＜0.05). The relative weights of the spleen and thymus to the body weight, serum HI antibody titers to H5-AI, H9-AI and ND, plasma GSH-Px and SOD activities were greater in immunosuppressed birds fed supplemented vitamin E (P＜0.05), and the MDA concentration was least at 50 mg•kg-1 supplemental vitamin E, and further additions had negative effect (P＜0.05). Birds treated with CTX fed diets with supplemental 100 and 200 mg vitamin E/kg had greater plasma PGE2, IL-1, IL-6, and TNF-α levels (P＜0.05). 【Conclusion】The results showed that dietary vitamin E supplementation significantly attenuated immunosuppressive effect of CTX challenge in laying hens.

Key words: vitamin E, laying hens, immunosuppression, mechanism

[1]Shini S, Huff G R, Shini A, Kaiser P. Understanding stress-induced immunosuppression: exploration of cytokine and chemokine gene profiles in chicken peripheral leukocytes. Poultry Science, 2010, 89: 841-851.

[2]He X, Yang X J, Guo Y M. Effects of different dietary oil sources on immune function in cyclophosphamide immunosuppressed chickens. Animal Feed Science and Technology, 2007, 139: 186-200.

[3]Sharma J M, Kim I J, Rautenschlein S, Yeh H Y. Infectious bursa disease virus of chickens: pathogenesis and immunosuppression. Developmental and Comparative Immunology, 2000, 24: 223-235.

[4]McConnell C D, Adair B M, McNulty M S. Effects of chichen anemia virus on macrophage function in chickens. Avian Diseases, 1993, 37: 358-365.

[5]高玉鹏. 透析我国蛋鸡产业面临的几个重大问题之五—免疫抑制已成为目前影响蛋鸡生产水平的重要障碍. 中国畜牧杂志, 2010, 46(4): 26-29.

Gao Y P. Overview problem for laying hens industry in China, 5. Immunosppression is the major inhibition of laying hens product. Animal Journal in China, 2010, 46(4): 26-29. (in Chinese)

[6]崔治中. 免疫抑制性病毒多重感染在鸡群疫病发生和流行中的作用. 畜牧兽医学报, 2003, 34(5): 417-421.

Cui Z Z. Effcct of multiple immunosuppressive viral infections on pathogenesis and epidemiology in chicken flocks. Jounal of Animal and Veterinary, 2003, 34(5): 417-421. (in Chinese)

[7]Ei-Abasy M, Motobu M, Nakamura K, Koge K, Onodera T, Vainio O, Toivanen P, Hirota Y. Preventive and therapeutic effects of sugar cane extract on cyclophosphamide induced immunosuppression in chickens. International Immunopharmacology, 2004, 4: 983-990.

[8]Bafna A R, Mishra S H. Protective effect of bioactive fraction of Sphaeranthus indicus Linn. againstcyclophosphamide induced suppression of humoral immunity in mice. Journal of Ethnopharmacology, 2006, 104: 426-429.

[9]李淑芳, 杨汉春, 张继东, 李  英, 刘会娟, 张晶晶. 米糠多糖对环磷酰胺诱导免疫抑制鸡外周血T淋巴细胞增殖活性及疫苗免疫效果的影响. 中国兽医科学, 2007, 37(7): 588-592.

Li S F, Yang H C, Zhang J D, Li Y, Liu H J, Zhang J J. Effects of rice bran polysaccharide on peripheral T lymphocyte proliferation activation and serum ND antibody titer after vaccination in immunosuppression chickens induced by cyclophosphamide. Veterinary Science in China, 2007, 37(7): 588-592. (in Chinese)

[10]柳风祥, 崔治中, 郭慧君. 复方中药提取物对REV诱发的免疫抑制鸡的免疫增强作用. 中国农业科学, 2009, 42(6): 2164-2171.

Liu F X, Cui Z Z, Guo H J. Immune enhancement effect of mixed herbal extracts in chicks immnunosuppressed by reticuloendotheliosis virus infection. Chinese Agricultural Science, 2009, 42(6): 2164-2171. (in Chinese)

[11]代  卉, 乐国伟, 孙  进, 韩  芳, 施用晖. 小麦肽对受环磷酰胺免疫抑制小鼠的免疫调节及抗氧化功能. 生物工程学报, 2009, 25(4): 549-553.

Dai H, Le G W, Sun J, Han F, Shi Y H. Immune modulation and antioxidant effects of wheat peptides on immunosuppressed mice. Chinese Journal of Biotechnology, 2009, 25(4): 549-553. (in Chinese)

[12]Zhang X, Zhong X, Zhou Y, Wang G, Du H, Wang T. Dietary RRR-α-tocopherol succinate attenuates lipopolysaccharide-induced inflammatory cytokines secretion in broiler chicks. British Journal of Nutrition, 2010, 104: 1796-1805.

[13]Niu Z Y, Liu F Z, Yan Q L, Li W C. Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress. Poultry Science, 2009, 88: 2101-2107.

[14]Zingg J M. Vitamin E: an overview of major research directions. Molecular Aspects of Medicine, 2007, 28: 400-422.

[15]Kirunda D F K, Scheideler S E, McKee S R. The ef?cacy of vitamin E(DL-α-tocopheryl acetate) supplementation in hen diets to alleviate egg quality deterioration associated with high temperature exposure. Poultry Science, 2001, 80: 1378-1383.

[16]Gao J, Lin H, Wang X J, Song Z G, Jiao H C. Vitamin E supplementation alleviates the oxidative stress induced by dexamethasone treatment and improves meat quality in broiler chickens. Poultry Science, 2010, 89: 318-327.

[17]Lin Y F, Tsai H L, Lee Y C, Chang S J. Maternal vitamin E supplementation affects the antioxidant capability and oxidative status of hatching chicks. The Journal of Nutrition, 2005, 135: 2457-2461.

[18]Boa-Amponsem K, Picard M, Blair M E, Meldrum B, Siegel P B. Memory antibody responses of broiler and leghorn chickens as influenced by dietary vitamin E and route of sheep red blood cell administration. Poultry Science, 2006, 85: 173-177.

[19]中华人民共和国农业部. 鸡饲养标准(NY/T 33-2004). 北京: 中国农业出版社, 2004.

Ministry of Agriculture of the People’s Republic of China. Chicken Feed Standard(NY/T 33-2004). Beijing: China Agriculture Press, 2004. (in Chinese)

[20]陈桂银. 饲料分析与检测. 北京: 中国农业大学出版社, 2008.

Chen G Y. Feed Analysis and Detection. Beijing: China Agriculture University Press, 2008. (in Chinese)

[21]郑明球. 家畜传染病学实验指导(兽医专业用). 第3版. 北京: 中国农业出版社, 2006.

Zheng M Q. Animal Infective Diseases Experiment Method(Used in Veterinary Major). 3rd ed. Bingjing: China Agriculture Press, 2006. (in Chinese)

[22]汪士奎. 免疫抑制对肉仔鸡营养代谢影响的研究[D]. 北京: 中国农业科学院, 2003.

Wang S K. The effect of immunosuppression on nutrition metabolism in broilers[D]. Beijing: Chinese Academy of Agriculture Sciences, 2003. (in Chinese)

[23]Hou F X, Yang H F, Yu T, Chen W. The immunosuppressive effects of 10 mg/kg cyclophosphamide in Wistar rats. Environmental Toxicology and Pharmacology, 2007, 24: 30-36.

[24]董晓芳, 汪仕奎, 萨仁娜, 张  琪, 佟建明. 免疫抑制剂环磷酰胺对肉仔鸡生产性能及其内分泌的影响. 畜牧兽医学报, 2007, 38(9): 993-998.

Dong X F, Wang S K, Sa R N, Zhang Q, Tong J M. Effect of immunosuppressant cyclophosphamide on performance and Incretion in broiler. Jounal of Animal and Veterinary, 2007, 38(9): 993-998. (in Chinese)

[25]Lo Fiego D P, Santoro P, Macchioni P, Mazzoni D, Piattoni F, Tassone F, De Leonibus E. The effect of dietary supplementation of vitamins C and E on the a-tocopherol content of muscles, liver and kidney, on the stability of lipids, and on certain meat quality parameters of the longissimus dorsi of rabbit. Meat Science, 2004, 67: 319-327.

[26]Hatfield P G, Robinson B L, Minikhiem D L, Kott R W, Roth N I, Daniels J T, Swenson C K. Serum α-tocopoherol and immune function in yearling ewes supplemented with zinc and vitamin E. Journal of Animal Science, 2002, 80: 1329-1334.

[27]Gore A B, Qureshi M A. Enhancement of humoral and cellular immunity by vitamin E after embryonic exposure. Poultry Science, 1997, 76: 984-991.

[28]Lin Y F, Chang S J, Hsu A L. Effects of supplemental vitamin E during the laying period on the reproductive performance of Taiwan native chickens. British Poultry Science, 2004, 45: 807-814.

[29]Leshchinsky T V, Klasing K C. Relationship between the level of dietary vitamin E and the immune response of broiler chickens. Poultry Science, 2001, 80: 1590-1599.

[30]Matar P, Rozados V R, Gervasoni S I, Scharovsky O G. Down regulation of T-cell-derived IL-10 production by low-dose cyclophosphamide treatment in tumor-bearing rats restores in vitro normal lymphoproliferative response. International Immunopharmacology, 2001, 1: 307-319.

[31]Puthpongsiriporn U, Scheideler S E, Sell J L, Beck M M. Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress. Poultry Science, 2001, 80: 1190-1200.

[32]Abdukalykova S T, Zhao X, Ruiz-Feria C A. Arginine and vitamin E modulate the subpopulations of T lymphocytes in broiler chickens. Poultry Science, 2008, 87: 50-55.

[33]Senthilkumar S, Yogeeta S K, Subashini R, Devaki T. Attenuation of cyclophosphamide induced toxicity by squalene in experimental rats. Chemico-Biological Interactions, 2006, 160: 252-260.

[34]曹志玲. 环磷酰胺对雏鸡血液超氧化物歧化酶(SOD)活性的影响. 广东畜牧兽医科技, 2007, 32(5): 36-37, 47.

Cao Z L. The effect of cyclophosphamide on plasma SOD activity in chicks. Guangdong Animal and Veterinary Science and Technology, 2007, 32(5): 36-37, 47. (in Chinese)

[35]Narciso-Gaytán C, Shin D, Sams A R, Bailey C A, Miller R K, Smith S B, Leyva-Ovalle O R, Sánchez-Plata M X. Soybean, palm kernel, and animal-vegetable oils and vitamin E supplementation effect on lipid oxidation stability of sous vide chicken meat. Poultry Science, 2010, 89: 721-728.

[36]Franchini A, Sirri F, Tallarico N, Minelli G, Iaffaldano N, Meluzzi A. Oxidative stability and sensory and functional properties of eggs from laying hens fed supranutritional doses of vitamins E and C. Poultry Science, 2002, 81: 1744-1750.

[37]MacDonald-Wicks L K, Garg M L. Vitamin E supplementation in the mitigation of carbon tetrachloride induced oxidative stress in rats. Journal of Nutritional Biochemistry, 2003, 14: 211-218.

[38]Beytut E, Aksakal M. Effects of dietary vitamin E and selenium on antioxidative defense mechanisms in the liver of rats treated with high doses of glucocorticoid. Biological Trace Element Research, 2003, 91: 231-241.

[39]Musalmah M, Fairuz A H, Gapor M T, Ngah W Z. Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats. Asia Pacific Journal of Clinical Nutrition (Suppl. 7), 2002, 11: 448-451.

[40]Eder K, Flader D, Hirche F, Brandsch C. Excess dietary vitamin E lowers the activities of antioxidative enzymes in erythrocytes of rats fed salmon oil. The Journal of Nutrition, 2002, 132: 3400-3404.

[41]Meydani M, Macauley J B, Blumberg J B. Effect of dietary vitamin E and selenium on susceptibility of brain regions to lipid peroxidation. Lipids, 1988, 23: 405-409.

[42]Chow C K, Reddy K, Tappel A L. Effect of dietary vitamin E on the activities of the glutathione peroxidase system in rat tissues. The Journal of Nutrition, 1973, 103: 618-624.

[43]Chen J Y, Latshaw I D, Lee H O, Min D B. α-Tocopherol content and oxidative stability of egg yolk as related to dietary α-tocopherol. Journal of Food Science, 1998, 63: 919-922.

[44]Yang N Y, Macdonald I B, Desai I D, Lee M. Vitamin E supplementation and glutathione peroxidase activity. Proceedings of the Society for Experimental Biology and Medicine, 1976, 151: 770-774.

[45]刘倚帆, 韩菲菲, 谢永刚, 汪以真. 重组猪乳铁蛋白N端多肽对环磷酰胺免疫抑制小鼠免疫功能的影响. 中国畜牧杂志, 2009, 45(21): 26-29.

Liu Y F, Han F F, Xie Y G, Wang Y Z. Effects of recombined procine lactofer r in-N on immunological function in immunosuppressive mice induced by cyclophosphamide. Journal of Animal Science in China, 2009, 45(21): 26-29. (in Chinese)

[46]高  芃, 钱嘉林, 刘长喜, 严卫星. 环磷酰胺对小鼠免疫抑制的动物模型建立. 环境与职业医学, 2004, 21(4): 314-318.

Gao P, Qian J L, Liu C X, Yan W X. Establishment of an immunosuppressive animal model induced by cyclophosphamide in mice. Journal of Environment Occupation Medicine, 2004, 21(4): 314-318. (in Chinese)

[47]Markowski-Grimsrud C J, Schat K A, Infection with chicken anaemia virus impairs the generation of pathogen-speci?c cytotoxic T lymphocytes. Immunology, 2003, 109: 283-294.

[48]Crowley T M, Haring V R, Moore R, Robert M. Chicken anemia virus: an understanding of the in vitro host response over time. Viral Immunology, 2011, 24(1): 3-9.

[49]李慧英. 壳聚糖对肉鸡免疫功能的影响及其分子机理研究[D]. 呼和浩特: 内蒙古农业大学, 2009.

Li H Y. Effects of chitosan on immune functions in broiler chickens and the underlying molecular mechanisms[D]. Huhhot: Inner Mongolia Agriculture University, 2009. (in Chinese)
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