Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4945-4951.doi: 10.3864/j.issn.0578-1752.2011.23.023

• RESEARCH NOTES • Previous Articles     Next Articles

Sequence Analysis of ITS Gene in Several Amblyseiinae (Acari: Phytoseiidae)

 ZOU  Zhi-Wen, CHEN  Fen, XIA  Bin, WU  Yu, ZHANG  Yu   

  1. 1.南昌大学生命科学与食品工程学院，南昌 330031
  • Received:2011-01-17 Online:2011-12-01 Published:2011-04-15

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Abstract: 【Objective】The objective of this study is to analyze the phylogenetic relationships among phytoseiid mites in the citrus orchards by comparing their ITS gene fragments, finally improve the classification system of phytoseiid mites. 【Method】The fragment sequences of ITS from 4 phytoseiid mites, Euseius nicholsi, Amblyseius eharai, A. tsugawai and A. orientalis, which collected from citrus orchards in Jiangxi province were analyzed by using softwares.【Result】Length of ITS from the 4 species are from 644-655 bp. The average A+T content is 58.2%. There are 204 variable sites and 32 insertion/deletion in these sequences. Results demonstrated that ITS2 regions are much more conserved than ITS1. Aligned with the ITS sequence of other 5 phytoseiid species of citrus orchard from GenBank, data analysis shows that the relationship between E. nicholsi and E. ovalis is closer than others, their genetic distance is 0.013. Four species of Neoseiulus and 3 species of Amblyseius are clustered into the one branch in the phylogenetic tree, and the nearest genetic distance, which between N. cucumeris and A. orientali, is 0.083.【Conclusion】The results support the taxonomic status of E. nicholsi and E. ovalis, and show sufficient evidence that Neoseiulus and Amblyseius should not be assigned to two genera, which the taxonomic system needs to be further researched.

Key words: Amblyseiinae, ITS, sequence analysis

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