Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4952-4961.doi: 10.3864/j.issn.0578-1752.2011.23.024

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of Tomato GMPase Overexpression on Tolerance of Potato Plants to Temperature Stress

 LI  Chao-Han, LI  Qing-Zhu, SHI  Qing-Hua, BAI  Long-Qiang, GUO  Xiao-Qing, LI  Xia, YU  Xian-Chang   

  1. 1.中国农业科学院蔬菜花卉研究所，北京100081
    2.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安271018
  • Received:2011-05-06 Online:2011-12-01 Published:2011-09-20

PDF

664

Cited

1

Abstract: 【Objective】 The aim of the study was to clarify the function of the GDP-D-mannose pyrophosphorylase (GMPase) in the L-galactose pathway of ascorbic acid (AsA) synthesis in plants, and to make sure the relation of GMPase overexpression with ascorbic acid content and temperature-stress tolerance ability. 【Method】 Two transgenic potato strains of overexpressing tomato GMPase were obtained by agrobacterium-mediated transformation. The transgenic plants and wild-type plants were treated in temperature-stress conditions (10/5℃ as low-temperature stress, 35/30℃ as high-temperature stress) for 3 days then in the recover-condition (25/20℃) for 3 days. Real time RT-PCR was used to analyze the expression of GMPase gene. And ascorbic acid content and relative index to temperature stress were also determined. 【Result】Compared with wild-type plants, with the overexpression of GMPase gene in transgenic plants, the GMPase activity and contents of AsA and dehydroascorbate (DHA) in normal and temperature-stress conditions were increased as well as the activities of dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and ascorbate peroxidase (APX). Meanwhile, the malondialdehyde (MDA) and H2O2 contents and permeability were decreased in the transgenic plants. 【Conclusion】 The overexpression of GMPase may increase potato tolerance to temperature stress by increasing AsA level and lowering reactive oxygen species (ROS).

Key words: potato, ascorbic acid, GDP-D-mannose pyrophosphorylase, transgenic, temperature stress

[1]Scandalios J G. Oxygen stress and superoxide dismutases. Plant Physiology, 1993, 101: 712-720.

[2]Asada K. Ascorbate peroidase: a hydrogen perioxide-scavenging enzyme in plants. Physiologia Plantarum, 1992, 85: 235-241.

[3]Elstner E F, Osswald W. Mechanisms of oxygen activation during plant stress. Proceedings of the Royal Society of Edinburgh, 1994, 102: 131-154.

[4]陈坤明, 宫海军, 王锁民. 植物抗坏血酸的生物合成、转运及其生物学功能.西北植物学报, 2004, 24(2): 329-336.

Chen K M, Gong H J, Wang S M. Biosynthesis, transport and function of ascorbates in plants. Northwest Plant Journal, 2004, 24(2): 329-336. (in Chinese)

[5]Manisha G, Ann C, Jaco V, Herman C. Copper affects the enzymes of ascorbate-glutathione cycle and its related metabolites in the roots of Phaseolus vulgaris. Physiologia Plantarum, 1999, 106(3): 262-267.

[6]Drazkiewicz M, Sko′rzyn′Ska-Polite E, Krupa Z. Response of the ascorbate-glutathione cycle to excess copper in Arabidopsis thaliana. Plant Science, 2003, 164: 195-202.

[7]Pukacka S, Ratajczak E. Antioxidative response of ascorbate- glutathione pathway enzymes and metabolites to desiccation of recalcitrant Acer saccharinum seeds. Journal of Plant Physiology, 2006, 163: 1259-1266.

[8]Zou L P, Li H X, Ouyang B, Zhang J H, Ye Z B. Cloning, expression, and mapping of GDP-D-mannose pyrophosphorylase cDNA from tomato (Lycopersicon esculentum L.). Acta Genetica Sinica, 2006, 33 (8): 757-764.

[9]Conklin P L, Norris S R, Wheeler G L , Williams E H, Smirnoff N, Last R L. Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96: 4198-4203.

[10]Huang C, He W, Guo J, Su P, Zhang L. Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant. Journal of Experimental Botany, 2005, 56: 3041-3049.

[11]Badejo A A, Jeong S T, Goto-Yamamoto N, Esaka M. Cloning and expression of GDP-D-mannose pyrophosphorylase gene and ascorbic acid content of acerola (Malpighia glabra L.)fruits at ripening stages. Plant Physiology and Biochemistry, 2007, 45:665-672.

[12]Keller R, Springer F, Renz R, Kossmann J. Antisense inhibition of the GDP-mannose pyrophosphorylase reduces the ascorbate content in transgenic plants leading to developmental changes during senescence. The Plant Journal, 1999, 19:131-141.

[13]Lin L L, Shi Q H, Wang H S, Qin A G, Yu X C. Over-expression of tomato GDP-Mannose pyrophosphorylase (GMPase) in potato increases ascorbate content and delays plant senescence. Agricultural Sciences in China, 2011, 10(4): 101-105.

[14]Nicot N, Hausman J F, Hoffmann L, Evers D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. Journal of Experimental Botany, 2005, 56: 2907-2914.

[15]Bubner B, Baldwin I T. Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Reports, 2004, 23: 263-27.

[16]Takahama U. Regulation of peroxidase-dependent oxidation of phenolics by ascorbic acid: Different effects of ascorbic acid on the oxidation of coniferyl alcohol by the apoplastic soluble and cell wall-bound peroxidases from epicotyls of Vignaangularis. Plant and Cell Physiology, 1994, 34:809-817.

[17]Chen Z, Young T E, Ling J, Chang S C, Gallie D R. Increasing vitamin C content of plants through enhanced ascorbate recycling. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100: 3525-3530.

[18]Jiménez A, Hernández J A, Rio L A, Sevilla F. Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves. Plant Physiology, 1997, 114: 275-284.

[19]Pignocchi C, Kiddle G, Hernández I, Foster S J,  Asensi A, Tahar Taybi T,  Barnes J,  Foyer C H. Ascorbate oxidase-dependent changes in the redox state of the apoplast modulate gene transcript accumulation leading to modified hormone signaling and orchestration of defense processes in tobacco. Plant Physiology, 2006, 141: 423-435.

[20]赵世杰, 许长成, 邹  琦. 植物组织中丙二醛测定方法的改进. 植物生理学通讯, 1994, 30(3): 207-210.

Zhao S J, Xu C C, Zou Q. Improvements of method for measurement of malondialdehyde in plant tissues. Plant Physiology Communications, 1994, 30(3): 207-210. (in Chinese)

[21]Petrie S E, Jaekson T L. Effects of nitrogen fertilization on manganese concentration and yield of barley and oats. Soil Science Society of America Journal, 1984, 48: 319-322.

[22]Wheeler G L, Jones M A, Smirnoff N. The biosynthetic pathway of vitamin C in higher plants. Nature, 1998, 393: 365-369.

[23]Smirnoff  N, Wheeler G L. Ascorbic acid in plants: biosynthesis and function. Critical Reviews in Biochemistry and Plant Molecular Biology, 2000, 35:291-314.

[24]Noctor G, Foyer C H. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 1998, 49:249-279.

[25]Badejo A A, Tanaka N, Esaka M. Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra L.) and increase in ascorbate content of transgenic tobacco expressing acerola gene. Plant and Cell Physiology, 2008, 49:126-132.

[26]秦爱国, 高俊杰, 于贤昌. 温度胁迫对马铃薯叶片抗坏血酸代谢系统的影响. 应用生态学报, 2009, 20(12): 2964-2970.

Qin A G, Gao J J, Yu X C. Effects of high and low-temperature stresses on ascorbic acid metabolism system in potato leaves.  Chinese Journal of Applied Ecology, 2009, 20(12): 2964-2970. (in Chinese)

[27]Liebler D C, Kling D S, Reed D J. Antioxidants protection of phospholipid bilayer by α-tocopherol. Control of α-tocopheol status and lipid peroxidation by ascorbic acid and glutathione. Journal of Biological Chemistry, 1986, (26):12114-12119.

[28]Tokunaga T, Miyahara K, Tabata K, Esaka M. Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for L-galactono-1, 4-lactone dehydrogenase. Plantarum, 2005, 220: 854-863.

[29]Smirnoff N, Colville L. Antioxidant status, peroxidase activity, and PR protein transcript levels in ascorbate-deficient Arabidopsis thaliana vtc mutants. Journal of Experimental Botany, 2008, 59(14): 3857-3868.

[30]Arial D, Jounechani R S. Impact of exogenous ascorbic acid on antioxidant activity and some physiological traits of common bean subjected to salinity stress. Notulae Botanicae Horti Agrobotanici Cluj- Napoca, 2009, 37(2):165-172.

[31]谢居清, 李国学, 王效科, 郑启伟, 冯兆忠. 外源抗坏血酸对臭氧胁迫下水稻光合及生长参数的影响. 中国生态农业学报, 2009, 17(6):1176-1181.

Xie J Q, Li G X, Wang X K, Zheng Q W, Feng Z Z. Effect of exogenous ascorbic acid on photosynthesis and growth of rice under O3 stress. Chinese Journal of Eco-Agriculture, 2009, 17(6):1176-1181. (in Chinese)

[32]Shigeoka S, Ishikawa T, Tamoi M, Yabuta Y, Yoshimura K. Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany, 2002, 53: 1305-1319.

[33]Yoshimura K, Yabuta Y, Tamoi M. Alternatively spliced mRNA variants of chloroplast ascorbate peroxidase isoenzymes in spinach leaves. Biochemistry, 1999, 338: 41-48.

[34]Eltayeb A E, Kawano N, Badawi G H. Enhanced tolerance to   ozone and drought stresses in transgenic tobacco overexpressing dehydroascorbate reductase in cytosol. Physiologia Plantarum, 2006, 127: 57-65.

[35]Yoon H S, Lee H, Lee I A, Kim K Y, Jo J. Molecular cloning of the monodehydroascorbate reductase gene from Brassica campestrsi and analysis of its mRNA level in response to oxidative stress. Biochimica and Biophysica Acta, 2004, 1658: 181-186.

[36]马春花, 马锋旺, 李明军, 束怀瑞. 外源抗坏血酸对离体苹果叶片衰老的影响. 园艺学报, 2006, 33(6):1179-1184.

Ma C H, Ma F W, Li M J, Shu H R. Effects of exogenous ascorbic acid on senescence of detached apple leaves. Acta Horticulturae Sinica, 2006, 33(6):1179-1184. (in Chinese)

[37]Ma Y H, Ma F W, Zhang J K, Li M J, Wang Y H, Dong L. Effects of high temperature on activities and gene expression of enzymes involved in ascorbate-glutathione cycle in apple leaves. Plant Science, 2008, 175:761-766.
[1] LI ShaoXing, SONG WenFeng, WEI ZeYu, ZHOU YuLing, SONG LiXia, REN Ke, MA Qun, WANG LongChang. Effects of Straw and Milk Vetch Mulching on Soil Fertility and Sweet Potato Yield [J]. Scientia Agricultura Sinica, 2025, 58(8): 1591-1603.
[2] MENG Hui, LUO BingYu, LU ZhengYu, WANG Peng, KANG DongRu, ZHENG ChengShu, WANG WenLi. Cloning of CmASMT and Its Role in Thermotolerance of Chrysanthemum [J]. Scientia Agricultura Sinica, 2025, 58(8): 1617-1626.
[3] YUE RunQing, LI WenLan, DING ZhaoHua, MENG ZhaoDong. Molecular Characteristics and Resistance Evaluation of Transgenic Maize LD05 with Stacked Insect and Herbicide Resistance Traits [J]. Scientia Agricultura Sinica, 2025, 58(7): 1269-1283.
[4] TANG Yu, LEI BiXin, WANG ChuanWei, YAN XuanTao, WANG Hao, ZHENG Jie, ZHANG WenJing, MA ShangYu, HUANG ZhengLai, FAN YongHui. Response Mechanism of Anthocyanin Accumulation in Colored Wheat to Post-Anthesis High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(6): 1083-1101.
[5] SU Ming, LI FanGuo, HONG ZiQiang, ZHOU Tian, LIU QiangJuan, BAN WenHui, WU HongLiang, KANG JianHong. Antioxidant Characterization of Nitrogen Application for Mitigating Potato Senescence Post-Flowering Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(4): 660-675.
[6] ZHENG Yu, CHEN Yi, TI JinSong, SHI LongFei, XU XiaoBo, LI YuLin, GUO Rui. Evaluation of Carbon Footprint and Economic Benefit of Different Tobacco Rotation Patterns [J]. Scientia Agricultura Sinica, 2025, 58(4): 733-747.
[7] MA HeXiao, GE GuoLong, ZHANG XiangQian, LU ZhanYuan, WANG ManXiu, RONG MeiRen, SHI JingJing, ZHANG DeJian, SUN XuePing. Effects of Different Crop Rotation Systems on Soil Readily Oxidized Organic Carbon and Carbon Pool Activity Differences [J]. Scientia Agricultura Sinica, 2025, 58(24): 5201-5215.
[8] DING Ning, QI EnFang, JIA XiaoXia, HUANG Wei, MA LiRong, LI JianWu, YAN RuNan. Screening and Identification of miRNAs in Potato Seedlings in Response to High Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(22): 4589-4602.
[9] DU TaiFeng, ZHOU YuanYuan, QIN Zhen, LI AiXian, WANG QingMei, ZHANG LiMing, HOU FuYun. Exogenous Brassinolide Alleviates the Inhibitory Effect of SPVD on Sweet Potato Storage Roots Germination [J]. Scientia Agricultura Sinica, 2025, 58(22): 4628-4637.
[10] CHEN YongXian, CHEN RuiJiang, DU YiZhi, ZHU JunJie, CHEN WanXia, ZHAO ZiHan, WANG JiChun, DU Kang, ZHANG Kai. Screening and Identification of Drought-Tolerant Sweet Potato Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(2): 214-237.
[11] LEI BiXin, YU YongBo, ZHANG MingTong, CUI GuoJi, HONG JiaWen, HU Tao, YOU AiXin, ZHANG WenJing, MA ShangYu, HUANG ZhengLai, FAN YongHui. Impact of Post-Anthesis Heat Stress on Nitrogen Use Efficiency and Yield Components in Wheat [J]. Scientia Agricultura Sinica, 2025, 58(19): 3837-3856.
[12] ZHAO DongLan, MA JuKui, XIAO ShiZhuo, ZHOU ZhiLin, ZHAO LingXiao, WANG Jie, DAI XiBin, SUN HouJun, CAO QingHe. QTL Analysis for Resistance to Stem Nematode Disease in Sweetpotato [J]. Scientia Agricultura Sinica, 2025, 58(17): 3389-3399.
[13] MA Jia, PENG JieLi, WANG Xu, JIA Nan, LI MengKai, HU Dong. Effects of Streptomyces sp. TOR3209 on Chlorophyll Synthesis and Polyamine Content in Tomato Plants Under Low Temperature Stress [J]. Scientia Agricultura Sinica, 2025, 58(15): 3064-3080.
[14] XIE HuiHui, YANG QiuHua, LI WenLi, ZHU JinCheng, LI HuiXia, ZHANG Feng. Identification of Wild Potato Introgression Lines Resistant to Southern Root-Knot Nematode [J]. Scientia Agricultura Sinica, 2025, 58(14): 2924-2932.
[15] ZHAO TianTian, YUAN JianLong, ZHUO FengQi, TANG ZhenSan, XU Jie, ZHANG Feng. Comprehensive Evaluation of Potato Flour Quality and Variety Screening [J]. Scientia Agricultura Sinica, 2025, 58(13): 2522-2537.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd