Abstract:

【Objective】 This research was aimed to explore a protocol of isolation, culture and identification of skeletal satellite cells, and to establish an optimal in vitro amplification system for Beijing Fatty chicken skeletal muscle satellite cells, thereby laying a technical foundation for future pertinent researches. 【Method】 Pectoral muscles were isolated from 15-day-old chicken embryos, disassociated with collagenase and trypsin and purified via differential adhesion. Satellite cells were detected for the expression of Pax7, Myod and Desmin and induced to muscle. Proliferative capacities in three different culture systems were compared as well. 【Result】 The cultured cells expressed Pax7 and MyoD in nucleus and desmin in cytoplasm, demonstrating that they were indeed Beijing Fatty chicken skeletal muscle satellite cells. Satellite cells underwent muscle differentiation and formed multinucleated myotubes. Muscle differentiation markers MHC in cytoplasm were detected in these cells. And it represented an optimal system for Beijing Fatty chicken skeletal muscle satellite cells to be cultured in media containing DMEM/F12+ 15%FBS+2.5ng?mL-1 basic fibroblast growth factor (bFGF). 【Conclusion】 The present study successfully isolated and identified the skeletal muscle satellite cells in Beijing Fatty chicken and established their optimal in vitro culture system. Satellite cells were also successfully induced to differentiate into muscle , and, accordingly, provided a theoretical and technical basis for studying the mechanism of skeletal muscle growth and development in future.

Key words: Beijing Fatty chicken, skeletal muscle satellite cells, identification, induction differentiation