DNA甲基化,干旱胁迫,甲基化敏感扩增多态性,高效液相层析," /> DNA甲基化,干旱胁迫,甲基化敏感扩增多态性,高效液相层析,"/> DNA methylation,drought stress,methylation sensitive amplified polymorphism,high performance liquid chromatography
,"/> <font face="Verdana">Spatial and Temporal Profiling of DNA Methylation Induced by Drought Stress in Rice#br# </font>

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (9): 3009-3018 .doi: 10.3864/j.issn.0578-1752.2009.09.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Spatial and Temporal Profiling of DNA Methylation Induced by Drought Stress in Rice#br#

PAN Ya-jiao,FU Bin-ying, WANG Di, ZHU Ling-hua,LI Zhi-kang#br#   

  1. (中国农业科学院作物科学研究所/国家农作物基因资源和遗传改良重大科学工程)
  • Received:2008-11-17 Revised:2009-01-15 Online:2009-09-10 Published:2009-09-10

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Abstract:

【Objective】 This experiment aimed at study of the relationship between DNA methylation and drought responsiveness in rice by characterizing DNA mathylation in leaves and roots at seedling stages and tillering stages under drought stress for DK106 (drought tolerance introgression line) and IR64 (recurrent parent and drought sensitive line). 【Method】 Methylation sensitive amplified polymorphism (MSAP) and high performance liquid chromatography (HPLC) were used to analyze the DNA methylation level in this study; The differentially methylated DNA fragments induced by drought were isolated from the polyacrylamide gel, cloned and sequenced, then the sequences were blasted at the NCBI website. 【Result】 Results showed that about 20% cytosine of CCGG sequences in rice genome was methylated, and the average level of methylation was increased under drought stress treatment, especially in roots; But the alterations of the DNA methylation state and level induced by drought stress were different between DK106 and IR64, and were also specific at different growth stages and in different tissues. The BLAST results of the differentially methylated DNA sequences showed that the methylation frequency was similar in coding region and non-coding region on the genome. 【Conclusion】 It was revealed that the pattern of DNA methylation under drought was temporal-spatial specific and variety specific, and the change of DNA methylation was related to drought responsiveness.

Key words: DNA methylation')">DNA methylation, drought stress, methylation sensitive amplified polymorphism, high performance liquid chromatography

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