Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (6): 1872-1878 .doi: 10.3864/j.issn.0578-1752.2008.06.040

• RESEARCH NOTES • Previous Articles    

Polymorphism of Bovine STAT5A Gene and its Associations with Milk Performance Traits in Chinese Holstein Cattle

  

  1. 徐州师范大学细胞与分子生物学研究所
  • Received:2006-10-31 Revised:2007-03-12 Online:2008-06-10 Published:2008-06-10

Abstract: PCR-SSCP was applied to analyze the polymorphisms of two locus in STAT5A gene in 279 Chinese Holstein cattle. Three genotypes were found at two locus and 7 haplotype combinations were present. The frequencies of genotype AA/BB/AB were 0.2401/0.1470/0.6129 and that of allele A/B in the population were 0.5466/0.4534 at locus P1. The result showed that the genotypes had significant effect on milk yield in the first lactations as also as protein rate in the second lactations. Genotype frequency of CC/DD/CD were 0.2401/0.1470/0.6129 and the frequencies of allele C/D in the population were 0.5466/0.4534 at locus P2. Different genotypes had remarkable influence on protein rate in the second lactations. In addition ,the frequencies of 7 haplotype combinations were 0.1864,0.4624,0.0502,0.1505,0.0430,0.1039 and 0.0036, different haplotype combinations affected milk yield, protein rate in the first and second lactations. No significant differences between individuals with different genotypes and haplotype combinations for milk fat rate in the first and second lactations at these two locus.

Key words: STAT5A, PCR-SSCP, Chinese Holstein cattle, Polymorphism, Haplotype

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