Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (5): 1524-1529 .doi: 10.3864/j.issn.0578-1752.2008.05.036

• VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Development of antibodies against Diethylstilbestrol

  

  1. 华南农业大学兽医学院药理研究室
  • Received:2007-04-09 Revised:2007-09-30 Online:2008-05-10 Published:2008-05-10

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Abstract: Four rabbit antisera against DES with high titers (>1∶1 000 000) were obtained by immunizing rabbits with DES immunizing antigen. One of them (No.4) was used to develop an indirect competitive inhibition enzyme-linked immunoassay (Ci-ELISA) for the determination of diethylstilbestrol (DES). Four calibration curves were made by KCjunior software according to 4-parameter model. In the linear range of 0.01-50μg/L, the IC50 was 0.71～1.0μg/L，R2 was 0.9952～0.9978。The coefficients of variation intra-assay and inter-assay were 0.52%-22.48%, 2.64%-19.51% respectively．The antiserum No.4 had cross reactivity of 8.5%, 38.5% with other two synthetic anabolic HEX, DIEN respectively. But had hardly cross reactivity with native estrogen E2, EE2 and Tyr.

Key words: DES, Polyclonal antiserum, Ci-ELISA

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