Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (10): 2169-2176 .doi: 10.3864/j.issn.0578-1752.at-2006-8153

• TILLAGE & CULTIVATION·PHYSIOLOGY & ECOLOGY • Previous Articles     Next Articles

Development of 2-DE protocol for wheat leaf proteomics

  

  1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程
  • Received:2006-09-28 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10

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Abstract: 【Objective】To develope an efficient and higher resolution 2-DE procedure for future wheat leaf proteome research.【Method】Sample preparation is carried out according to two protocols modified from former researchers,following 2-DE and MS analysis. 【Result】These two protocols presented here are efficient protein extraction methods,and both are compatible with MS analysis.【Conclusion】The results indicate that both protein extraction protocols utilized are useful in wheat leaf proteome research.

Key words: wheat leaf, 2-DE, peptide mass fingerprinting, sample preparation, proteome

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