Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (03): 582-588 .

• HORTICULTURE • Previous Articles     Next Articles

Cytological and Molecular Studies on Genomic Exchange and Reconstitution in the Synthetic Allotetraploid Cucumis hytivus

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  1. 南京农业大学
  • Received:2004-04-16 Revised:2004-07-29 Online:2005-03-10 Published:2005-03-10

Abstract: The exchanges and reconstitutions between the two genomes in the allotetraploid Cucumis hytivus Chen & Kirkbride (2n = 4x = 38) were investigated by cytological and molecular means. Among the 108 pollen mother cells (PMC) observed, 50 PMC (about 46.3%) had multivalents, indicating the wide genomic exchange and reconstitution. The average chromosome configuration was 0.56I+17.36II+0.35III+0.26IV+0.046V+0.056VI. Among 446 arbitrary primers, only 5 primers could produce 6 specific bands of C. hytivus. Three of them were selected and converted into sequence characterized amplified region (SCAR) markers. The SCAR markers were used in amplification with 13 Cucumis genotypes, and the results showed that only SAP-03/700 marker was specifically amplified. Compared the sequence of 3 different bands amplified by SAP-03/700 primer pair in C. hytivus, C. sativus var. sativus and C. sativus var. hardwickii, the sequences at 2 ends were similar while the middle parts were different. In addition, about 200 bp of SAP-03/700 was homologous with cucumber mitochorndrial genome, but the directions were contrary.

Key words: Cucumis, Allotetroploid, Exchange and reconstitutions, Cytology, RAPD, SCAR

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