Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (9): 1943-1951 .doi: 10.3864/j.issn.0578-1752.at-2006-7846

• PLANT PROTECTION • Previous Articles     Next Articles

Studies on prunus necrotic ringspot Ilarvirus(Prunus necrotic ringspot virus,PNRSV) occurred on Lily

  

  1. 北京出入境检验检疫局
  • Received:2006-07-18 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10

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Abstract: 【Objective】Molecular Identification of Prunus necrotic ringspot virus occurred on Lily. 【Method】Lily corms were randomly selected and grown in greenhouse. Total RNAs were extracted from younger leaves with RNA Extraction Kit (Sangon, China), and RT-PCR amplification of CP cDNA fragments was performed with primer pairs specific for PNRSV. An expected cDNA fragment of about 450bp was harvested and cloned into pGEM-T-easy Vector. Clones were sequenced and compared with reported PNRSV isolates in Gene bank【Result】The nucleotide sequence of the cloned cDNA revealed that 450bp sequence is belong to PNRSV, and has a 84.5%~99.1% homology with 27 reported PNRSV isolates.【Conclusion】Identifying the occurrence of Prunus necrotic ringspot virus on Lily with molecular bio-methods, and confirming that lily is a new nature host of PNRSV.

Key words: Lily, Prunus necrotic ringspot virus, RT-PCR

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