Scientia Agricultura Sinica

Previous Articles    

Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage

GUO ShaoLei1, XU JianLan1, WANG XiaoJun1,2, SU ZiWen1,2, ZHANG BinBin1, MA RuiJuan1, YU MingLiang1* #br#   

  1. 1Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014; 2College of Horticulture, Nanjing Agricultural University, Nanjing 210095
  • Online:2022-08-21 Published:2022-08-21

PDF

44

Abstract: 【Objective】The aim of this study was to identify XTH genes family from peach, and to analyze the expression of PpXTHs in different texture peach fruit during storage, which not only provided data support for exploring the candidate PpXTHs involved in peach fruit softening, but also laid the foundation for further studying PpXTHs function in peach fruit softening.【Method】The HMM profiles of the Glyco_hydro_16 domain and XET_C domain were used to search all XTH proteins with the Hmmer 3.1 software in peach protein database. Then the molecular weight, theoretical isoelectric point and other physicochemical properties were predicted by online tool ProtParam. PpXTHs subcellular localization were predicted by online software Plant-mPLoc. The MEGA 11 software was used to construct phylogenetic tree. The online tool MEME was used to analyze conserved motif, and conserved protein domain and gene structure map were draw by Tbtools. According to the PpXTHs family gene location information, chromosome mapping was carried out in MapChart software. Then the expression of PpXTHs in different texture of peach fruit during storage were detected by qRT-PCR. 【Result】A total of 27 PpXTH genes were systematically identified from peach, which were distributed on 7 chromosomes. Based on the phylogenetic tree, ancestral group, Ⅰ/Ⅱ subfamily, ⅢA subfamily and ⅢB subfamily were classified. In addition, according to the analysis of protein domains, all PpXTHs gene family proteins had Glyco_hydro_16 and XET_C conserved domain. The results of qRT-PCR showed that PpXTH33 belonging to ⅢB subfamily was up-regulated with the extension of storage period in melting peach, and the expression was prominent higher than that in stony hard peach storage period. The positive clone sequencing was consistent with the CDS sequence of Prunus persica reference genome with the length of 924 bp coding 307 amino acid sequence. The PpXTH33 combined with GFP may mainly located in the cell wall and nucleus by confocal laser scanning microscopy. 【Conclusion】All 27 PpXTHs protein structures contained 2 highly XTH conserved domains and distributed on 7 chromosomes . The expression characteristics of PpXTH33 in different textures of peach fruit during storage suggested that PpXTH33 was closely related to peach fruit softening during storage.

Key words: Peach, Fruit softening, XTH gene family, Gene expression, Subcellular localization

[1] QU Qing, LIU Ning, ZOU JinPeng, ZHANG YaXuan, JIA Hui, SUN ManLi, CAO ZhiYan, DONG JinGao. Screening of Differential Genes and Analysis of Metabolic Pathways in the Interaction Between Fusarium verticillioides and Maize Kernels [J]. Scientia Agricultura Sinica, 2023, 56(6): 1086-1101.
[2] CAO Ke, CHEN ChangWen, YANG XuanWen, BIE HangLing, WANG LiRong. Genomic Selection for Fruit Weight and Soluble Solid Contents in Peach [J]. Scientia Agricultura Sinica, 2023, 56(5): 951-963.
[3] PENG JiaWei, ZHANG Ye, KOU DanDan, YANG Li, LIU XiaoFei, ZHANG XueYing, CHEN HaiJiang, TIAN Yi. Transcriptome Analysis of Peach Fruits at Different Developmental Stages in Peach Kurakato Wase and Early-Ripening Mutant [J]. Scientia Agricultura Sinica, 2023, 56(5): 964-980.
[4] ZOU Ting, LIU LiLi, XIANG JianHua, ZHOU DingGang, WU JinFeng, LI Mei, LI Bao, ZHANG DaWei, YAN MingLi. Cloning of MYBL2 Gene from Brassica and Its PCR Identification in Genomes A, B and C [J]. Scientia Agricultura Sinica, 2023, 56(3): 416-429.
[5] LIU ZhenShan, TU HongXia, ZHOU JingTing, MA Yan, CHAI JiuFeng, WANG ZhiYi, YANG PengFei, YANG XiaoQin, Kumail Abbas, WANG Hao, WANG Yan, WANG XiaoRong. Genetic Analysis of Fruits Characters in Reciprocal Cross Progenies of Chinese Cherry [J]. Scientia Agricultura Sinica, 2023, 56(2): 345-356.
[6] LIU SuNing, BIE HangLing, WANG JunXiu, CHEN XueJia, WANG XinWei, WANG LiRong, CAO Ke. Background Selection and Comparison of Marker Superiority and Inferiority of Aphid-Resistant Seedlings in an Interspecific Cross Peach Population [J]. Scientia Agricultura Sinica, 2023, 56(15): 2995-3005.
[7] WANG ZhaoHui, LI Yong, CAO Ke, ZHU GengRui, FANG WeiChao, CHEN ChangWen, WANG XinWei, WU JinLong, WANG LiRong. Genotype Identification and Combination Analysis of Loci Related to the Peach Flesh Texture Trait via 189 Peach Accessions [J]. Scientia Agricultura Sinica, 2023, 56(12): 2367-2379.
[8] ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[9] GU LiDan,LIU Yang,LI FangXiang,CHENG WeiNing. Cloning of Small Heat Shock Protein Gene Hsp21.9 in Sitodiplosis mosellana and Its Expression Characteristics During Diapause and Under Temperature Stresses [J]. Scientia Agricultura Sinica, 2023, 56(1): 79-89.
[10] YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang. Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites [J]. Scientia Agricultura Sinica, 2022, 55(6): 1149-1158.
[11] ZHANG JinLong,ZHAO ZhiBo,LIU Wei,HUANG LiLi. The Function of Key T3SS Effectors in Pseudomonas syringae pv. actinidiae [J]. Scientia Agricultura Sinica, 2022, 55(3): 503-513.
[12] SHU JingTing,SHAN YanJu,JI GaiGe,ZHANG Ming,TU YunJie,LIU YiFan,JU XiaoJun,SHENG ZhongWei,TANG YanFei,LI Hua,ZOU JianMin. Relationship Between Expression Levels of Guangxi Partridge Chicken m6A Methyltransferase Genes, Myofiber Types and Myogenic Differentiation [J]. Scientia Agricultura Sinica, 2022, 55(3): 589-601.
[13] GUO ShaoLei, XU JianLan, WANG XiaoJun, SU ZiWen, ZHANG BinBin, MA RuiJuan, YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[14] HAO Yan,LI XiaoYing,YE Mao,LIU YaTing,WANG TianYu,WANG HaiJing,ZHANG LiBin,XIAO Xiao,WU JunKai. Characteristics of Volatile Components in Peach Fruits of 21shiji and Jiucui and Their Hybrid Progenies [J]. Scientia Agricultura Sinica, 2022, 55(22): 4487-4499.
[15] KANG Chen,ZHAO XueFang,LI YaDong,TIAN ZheJuan,WANG Peng,WU ZhiMing. Genome-Wide Identification and Analysis of CC-NBS-LRR Family in Response to Downy Mildew and Powdery Mildew in Cucumis sativus [J]. Scientia Agricultura Sinica, 2022, 55(19): 3751-3766.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd