Scientia Agricultura Sinica ›› 2026, Vol. 59 ›› Issue (11): 2299-2313.doi: 10.3864/j.issn.0578-1752.2026.11.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening and Comprehensive Evaluation of Low Nitrogen Tolerance Germplasm Resources in Spring Wheat

GAO YaFeng1,2(), YANG JiaNing1,2, SUN XueDi1, CHEN JiaJia1, DANG ZhiJuan1,2, WANG JunCheng1,2, ZHANG Hong1,2, MA XiaoLe1,2, YAO LiRong1,2, MENG YaXiong1,2, SI ErJing1,2, LI BaoChun2,3, WANG HuaJun1,2()   

  1. 1 College of Agronomy, Gansu Agricultural University, Lanzhou 730070
    2 State Key Laboratory of Aridland Crop Science, Lanzhou 730070
    3 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070
  • Received:2025-10-31 Accepted:2025-12-29 Online:2026-06-01 Published:2026-06-03
  • Contact: WANG HuaJun

RichHTML

4

PDF

42

Abstract:

【Objective】Excessive dependence on nitrogen (N) fertilizer to achieve high crop yields in modern agricultural production has increased production costs and caused serious environmental problems, such as soil acidification and water eutrophication. Screening low-nitrogen-tolerant wheat cultivars is a key approach to improving nitrogen use efficiency and significantly reducing nitrogen fertilizer input. This study aimed to elucidate the low-nitrogen tolerance characteristics of spring wheat, provide germplasm resources, and establish a theoretical basis for gene mining and breeding of low-nitrogen-tolerant wheat varieties.【Method】A total of 285 spring wheat accessions were used as experimental materials. Seedling-stage germination bag experiments were conducted under low nitrogen (0.05 mmol·L-1) and normal nitrogen (5 mmol·L-1) treatments. Nine traits, including seedling length, root length, shoot dry weight, and root dry weight, were measured, and the low-nitrogen tolerance coefficient for each trait was calculated. A comprehensive evaluation of seedling-stage low-nitrogen tolerance was performed using principal component analysis and the membership function method. Selected materials were further subjected to precise field screening and identification. Representative low-nitrogen-tolerant and nitrogen-sensitive materials were used to analyze changes in nitrogen uptake, assimilation, and transport-related enzyme activities under low-nitrogen stress, as well as grain quality traits. 【Result】Based on comprehensive evaluation using principal component analysis and the membership function method, the 285 spring wheat accessions were classified into five groups. Group Ⅰ consisted of extremely nitrogen-sensitive materials (5 accessions) with D values ranging from 0.14 to 0.20, including WN-269, WN-247, WN-244, WN-149, and WN-249. Group Ⅱ included nitrogen-sensitive materials (84 accessions) with D values of 0.20 to 0.34. Group Ⅲ comprised moderately nitrogen-efficient materials (162 accessions) with D values of 0.34 to 0.51. Group Ⅳ consisted of low-nitrogen-tolerant materials (29 accessions) with D values of 0.51 to 0.61. Group Ⅴ included extremely low-nitrogen-tolerant materials (5 accessions) with D values of 0.61 to 0.70, namely WN-49, WN-186, WN-237, WN-294, and WN-235. Five extremely nitrogen-sensitive and five extremely low-nitrogen-tolerant accessions were selected for field identification, resulting in the final identification of one nitrogen-sensitive accession (WN-269) and one low-nitrogen-tolerant accession (WN-235). Analysis of nitrate reductase, glutamate synthase, and glutamine synthetase activities showed that enzyme activities in leaves significantly decreased under low-nitrogen stress, while enzyme activities in the low-nitrogen-tolerant material were significantly higher than those in the nitrogen-sensitive material.【Conclusion】One low-nitrogen-tolerant wheat accession and one nitrogen-sensitive accession were successfully identified. A comprehensive evaluation system for low-nitrogen tolerance at both seedling and adult stages in wheat was established, and root surface area, root volume, and root dry weight were identified as core indicators for evaluating low-nitrogen tolerance.

Key words: spring wheat, nitrogen deficiency stress, cluster analysis, integrated evaluation, nitrate reductase, glutamate synthase, glutamine synthetase

Table 1

Statistical analysis of nitrogen deficiency tolerance coefficients for agronomic traits of wheat at seedling stage"

性状
Trait		 变化范围
Range of variation		 平均值
Average		 标准差
Standard deviation		 变异系数
Coefficient of variation (%)
根长RL 0.62-1.85 1.16 0.21 18
苗长SL 0.61-1.43 0.96 0.12 13
地下部干重UDW 0.50-2.42 1.20 0.30 25
地上部干重ADW 0.38-2.43 0.95 0.26 28
根冠比RSDW 0.39-2.13 1.29 0.19 15
总根长TRL 0.48-2.57 1.15 0.31 27
根表面积RSA 0.45-2.27 1.13 0.31 28
根系平均直径RAD 0.64-1.92 1.00 0.15 15
根体积RV 0.44-2.16 1.13 0.32 28

Table 2

Load matrix, characteristics value and contribution rate of low nitrogen tolerance coefficient of each growth indicator of the tested spring wheat materials at seedling stage"

性状
Trait		 主成分Principal component
1 2 3 4
根长RL 0.590 -0.101 0.096 0.788
苗长SL 0.831 -0.197 -0.054 -0.147
地下部干重UDW 0.894 -0.129 0.241 -0.180
地上部干重ADW 0.877 -0.268 -0.239 -0.151
根冠比RSDW 0.016 0.317 0.939 -0.074
总根长TRL 0.903 -0.22 0.127 -0.051
根表面积RSA 0.924 0.259 -0.018 -0.003
根系平均直径RAD 0.195 0.901 -0.290 -0.001
根体积RV 0.815 0.432 -0.089 0.002
贡献率Contributive ration (%) 55.317 15.019 12.426 7.848
权重Weight 0.611 0.166 0.137 0.087

Fig. 1

Scatter plots of tolerance indices to low nitrogen conditions at seedling stage"

Fig. 2

Correlation coefficients between nitrogen stress to lerance index and comprehensive evaluation value (D) of wheat at seedling stage"

Table 3

Predicted optimal nitrogen deficiency tolerance model for wheat at seedling stage"

多元回归方程Multiple regression equation 决定系数R2 F值F value P值P value
D=0.067+0.289X1 0.865 1823.347 <0.01
D=0.023+0.213X1+0.108X2 0.912 1464.946 <0.01
D=0.015+0.156X1+0.111X2+0.062X3 0.922 1113.032 <0.01
D=-0.01+0.15X1+0.101X2+0.063X3+0.043X4 0.924 847.195 <0.01

Fig. 3

Dendrogram of nitrogen efficiency of wheat seedling sunder low nitrogen treatments The yellow area shows the extremely low nitrogen tolerant type, the purple area shows the low nitrogen tolerant type, and the green area shows the nitrogen medium effective type. The blue area shows the nitrogen sensitive type and the red area shows the nitrogen extremely sensitive type"

Fig. 4

D-value analysis of different low-nitrogen tolerant wheat types Ⅰ: The nitrogen extremely sensitive type; Ⅱ: The nitrogen sensitive type; Ⅲ: The nitrogen medium effective type; Ⅳ: The low nitrogen tolerant type; Ⅴ: The extremely low nitrogen tolerant type. * indicates a significant difference at P<0.05 level"

Table 4

Load matrix, characteristics value and contribution rate of low nitrogen tolerance coefficient of each growth indicator of the tested spring wheat materials at adult plant stage"

性状
Trait		 主成分Principal component
1 2 3
株高PH 0.602 -0.164 -0.725
穗长SL 0.746 0.313 0.162
有效分蘖ET 0.969 -0.101 -0.024
穗粒数GNPS 0.653 0.596 0.235
整株干重TPDW 0.778 -0.544 0.006
千粒重TSW 0.406 -0.726 0.387
株粒数GNPP 0.965 0.170 0.084
株粒重GWPP 0.840 0.470 -0.072
经济系数EC -0.289 0.923 -0.009
贡献率Contributive ration (%) 53.073 26.860 8.550
权重Weight 0.599 0.304 0.097

Table 5

Comprehensive evaluation of 10 wheat varieties under low nitrogen stress"

材料
Materials		 品种
Variety		 综合
值1
C1		 综合
值2
C2		 综合
值3
C3		 隶属值1 ψ(C1) 隶属值2 ψ(C2) 隶属值3 ψ(C3) 综合评价值 Comprehensive
assessment value (D)		 排序
Order
WN-269 54IBWSN (1136) -1.130 -0.703 0.244 0.099 0.135 0.518 0.150 1
WN-149 CY-0161 -0.629 -0.915 -0.129 0.287 0.068 0.411 0.232 2
WN-247 54IBWSN (1101) -1.394 1.263 0.066 0.000 0.757 0.467 0.275 3
WN-244 54IBWSN (1097) -0.860 -0.091 1.003 0.200 0.329 0.736 0.291 4
WN-294 54IBWSN (1172) -0.330 0.520 -0.060 0.398 0.522 0.430 0.439 5
WN-237 54IBWSN (1086) 0.431 -0.176 -1.560 0.683 0.302 0.000 0.501 6
WN-249 54IBWSN (1103) 0.459 -0.298 -1.299 0.694 0.263 0.075 0.503 7
WN-186 54IBWSN (1005) 1.033 -1.131 -0.277 0.909 0.000 0.368 0.580 8
WN-49 ZH871 1.276 -0.532 1.924 1.000 0.190 1.000 0.754 9
WN-235 54IBWSN (1083) 0.833 2.033 0.089 0.834 1.000 0.473 0.849 10

Fig. 5

Cluster analysis of low nitrogen tolerance in wheat at the adult stage Ⅰ: The nitrogen sensitive type; Ⅱ: The low nitrogen tolerant type"

Fig. 6

Differences in growth at seedling and mature stages, and grain morphology between WN-235 and WN-269 under different nitrogen treatments A: Root system of seedling stage after 30 days of hydroponic culture; B: Whole-plant of mature stage; C: Grain morphology. CK: Normal nitrogen level; LN: Low nitrogen stress. The same as below"

Fig. 7

Analysis of nitrate reductase, glutamine synthetase, and glutamate synthetase activities in leaves of WN-269 and WN-235 under low nitrogen stress and normal nitrogen supply conditions with different nitrogen treatments Different lowercase letters indicate significant differences at the P<0.05 level. The same as below"

Fig. 8

Changes of morphology and quality indicators of WN-269 and WN-235 under low nitrogen stress"

[1]
BARRACLOUGH P B, HOWARTH J R, JONES J, LOPEZ- BELLIDO R, PARMAR S, SHEPHERD C E, HAWKESFORD M J. Nitrogen efficiency of wheat: Genotypic and environmental variation and prospects for improvement. European Journal of Agronomy, 2010, 33(1): 1-11.

doi: 10.1016/j.eja.2010.01.005
[2]
朱明昆, 包俊浩, 庞菁璐, 周诗绮, 方忠艳, 郑文, 张亚洲, 吴丹丹. 纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F1的创制及鉴定. 作物学报, 2024, 50(6): 1406-1419.
ZHU M K, BAO J H, PANG J L, ZHOU S Q, FANG Z Y, ZHENG W, ZHANG Y Z, WU D D. Generation and identification of a resistance to stripe rust perennial intergeneric hybrid F1 between Roegneria ciliaris and common wheat. Acta Agronomica Sinica, 2024, 50(6): 1406-1419. (in Chinese)

doi: 10.3724/SP.J.1006.2024.31056
[3]
ERISMAN J W, SUTTON M A, GALLOWAY J, KLIMONT Z, WINIWARTER W. How a century of ammonia synthesis changed the world. Nature Geoscience, 2008, 1(10): 636-639.

doi: 10.1038/ngeo325
[4]
LIANG X J, AN W, LI Y K, WANG Y J, QIN X Y, CUI Y H, SU S C. Impact of different rates of nitrogen supplementation on soil physicochemical properties and microbial diversity in goji berry. Phyton, 2024, 93(3): 467-486.

doi: 10.32604/phyton.2024.047628
[5]
ZI W J, LI J Z, CHEN J N, CAO F B, ZHENG H B, WANG W Q, HUANG M. High grain yield and high nitrogen use efficiency can be achieved simultaneously in single-season hybrid rice. Journal of Soil Science and Plant Nutrition, 2025, 25(3): 7360-7367.

doi: 10.1007/s42729-025-02600-y
[6]
纪博翔, 王长远, 董军伟, 宋宜萱, 吕亚辉, 王禹凡, 张明明, 乔匀周. 不同施肥措施对盐碱地土下覆膜冬小麦生长及产量的影响. 西南农业学报, 2025, 38(10): 2238-2245.
JI B X, WANG C Y, DONG J W, SONG Y X, LÜ Y H, WANG Y F, ZHANG M M, QIAO Y Z. Effects of different fertilization measures on growth and yield of winter wheat under subsurface film mulching in saline-alkali soil. Southwest China Journal of Agricultural Sciences, 2025, 38(10): 2238-2245. (in Chinese)
[7]
DING C, PIERCE S, YANG G J, HU Y Y, ZHANG Z W, LÜ X T. Linking plant nitrogen use efficiency with single traits, ecological strategies and phylogeny in a temperate steppe. Plant and Soil, 2024, 503(1): 283-293.

doi: 10.1007/s11104-024-06583-0
[8]
ANAS M, LIAO F, VERMA K K, SARWAR M A, MAHMOOD A, CHEN Z L, LI Q, ZENG X P, LIU Y, LI Y R. Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency. Biological Research, 2020, 53(1): 47.

doi: 10.1186/s40659-020-00312-4 pmid: 33066819
[9]
CHOI H W. From the photosynthesis to hormone biosynthesis in plants. The Plant Pathology Journal, 2024, 40(2): 99-105.

doi: 10.5423/PPJ.RW.01.2024.0006
[10]
查文童, 朱志畅, 郭双霜, 葛焱. 基于文献计量学的小麦氮素监测研究进展分析. 农业工程学报, 2023, 39(23): 1-13.
ZHA W T, ZHU Z C, GUO S S, GE Y. A bibliometric-based analysis of research advances in the field of nitrogen monitoring in wheat. Transactions of the Chinese Society of Agricultural Engineering, 2023, 39(23): 1-13. (in Chinese)
[11]
齐学礼, 马杰, 赵明忠, 董海滨, 张煜, 许国震. 低氮胁迫对不同氮效率小麦品种氮积累、代谢和产量、品质的影响. 河南农业科学, 2024, 53(4): 30-36.
QI X L, MA J, ZHAO M Z, DONG H B, ZHANG Y, XU G Z. Effects of low nitrogen stress on nitrogen accumulation, metabolism, yield and quality of wheat varieties with different nitrogen efficiency. Journal of Henan Agricultural Sciences, 2024, 53(4): 30-36. (in Chinese)
[12]
李双双, 付驰, 孙继, 顾万荣, 芦玉双, 许为政, 李晶. 施氮量对春小麦根系生理活性及籽粒蛋白品质的影响. 麦类作物学报, 2012, 32(6): 1139-1143.
LI S S, FU C, SUN J, GU W R, LU Y S, XU W Z, LI J. Effects of nitrogen amount on root physiological activity and grain protein quality in spring wheat. Journal of Triticeae Crops, 2012, 32(6): 1139-1143. (in Chinese)
[13]
周晓明, 张志勇, 王小纯, 熊淑萍, 韩锦峰, 马新明. 不同氮效率小麦的氮代谢特征及GS酶活性与氮代谢指标的相关性研究. 河南农业科学, 2016, 45(9): 15-20, 32.
ZHOU X M, ZHANG Z Y, WANG X C, XIONG S P, MA X M. Studies on nitrogen metabolism characteristics of wheat varieties with different nitrogen use efficiency and correlation between GS activity and nitrogen metabolism markers. Journal of Henan Agricultural Sciences, 2016, 45(9): 15-20, 32. (in Chinese)
[14]
祁小平, 李广, 闫丽娟, 袁建钰, 杜梦寅, 庞烨. 耕作和施肥方式对陇中旱作春小麦氮素利用与硝态氮残留的影响. 麦类作物学报, 2023, 43(4): 477-486.
QI X P, LI G, YAN L J, YUAN J Y, DU M Y, PANG Y. Effects of tillage and fertilization methods on nitrogen use efficiency and nitrate residue of spring wheat in dry farming of central Gansu Province. Journal of Triticeae Crops, 2023, 43(4): 477-486. (in Chinese)
[15]
姜瑛, 戚秀秀, 李祥剑, 汪强, 谭金芳, 韩燕来, 李培培. 不同小麦品种的氮素利用特性研究. 麦类作物学报, 2019, 39(6): 702-708.
JIANG Y, QI X X, LI X J, WANG Q, TAN J F, HAN Y L, LI P P. Study on nitrogen utilization characteristics of different wheat cultivars. Journal of Triticeae Crops, 2019, 39(6): 702-708. (in Chinese)
[16]
SINGH R, UPADHYAYS K, AGGARWAL, DIWAKAR, SHARMA I, PRASAD N. A study on hydroponic farming system of wheat, spinach and sword lily for sustainable development of agriculture. Bio Science Research Bulletin, 2019, 35(2): 59-63.

doi: 10.5958/2320-3161.2019.00014.2
[17]
WANG J, SUN J H, MIAO J, GUO J K, SHI Z L, HE M Q, CHEN Y, ZHAO X Q, LI B, HAN F P, et al. A phosphate starvation response regulator Ta-PHR1 is involved in phosphate signalling and increases grain yield in wheat. Annals of Botany, 2013, 111(6): 1139-1153.

doi: 10.1093/aob/mct080 pmid: 23589634
[18]
黄兴法, 孙旻昊, 刘博. 甘肃民勤地区春小麦长畦田分段灌溉与波涌灌溉技术适应性田间试验研究. 农业工程, 2021, 11(10): 73-79.
HUANG X F, SUN M H, LIU B. Field experiment on adaptability of border irrigation for long border field with segmented irrigation and surge irrigation of spring wheat in Minqin area of Gansu Province. Agricultural Engineering, 2021, 11(10): 73-79. (in Chinese)
[19]
赵瑞, 张旭辉, 张程炀, 郭泾磊, 汪妤, 李红霞. 小麦种质资源成株期氮效率评价及筛选. 中国农业科学, 2021, 54(18): 3818-3833. doi: 10.3864/j.issn.0578-1752.2021.18.003.
ZHAO R, ZHANG X H, ZHANG C Y, GUO J L, WANG Y, LI H X. Evaluation and screening of nitrogen efficiency of wheat germplasm resources at mature stage. Scientia Agricultura Sinica, 2021, 54(18): 3818-3833. doi: 10.3864/j.issn.0578-1752.2021.18.003. (in Chinese)
[20]
宋晓, 张珂珂, 黄晨晨, 黄绍敏, 郭斗斗, 岳克, 张水清. 基于主成分分析的氮高效小麦品种的筛选. 河南农业科学, 2020, 49(12): 10-16.
SONG X, ZHANG K K, HUANG C C, HUANG S M, GUO D D, YUE K, ZHANG S Q. Selection of nitrogen-efficient wheat varieties based on principal component analysis. Journal of Henan Agricultural Sciences, 2020, 49(12): 10-16. (in Chinese)
[21]
徐波, 林先意, 安瞳昕, 华为, 王卫东, 朱靖环, 范敏. 小麦种质资源氮素吸收力评价与筛选. 分子植物育种, 2025, 23(10): 3329-3338.
XU B, LIN X Y, AN T X, HUA W, WANG W D, ZHU J H, FAN M. Evaluation and screening of nitrogen absorption capacity of wheat germplasm resources. Molecular Plant Breeding, 2025, 23(10): 3329-3338. (in Chinese)
[22]
连盈, 卢娟, 胡成梅, 牛胤全, 史雨刚, 杨进文, 王曙光, 张文俊, 孙黛珍. 低氮胁迫对谷子苗期性状的影响和耐低氮品种的筛选. 中国生态农业学报(中英文), 2020, 28(4): 523-534.
LIAN Y, LU J, HU C M, NIU Y Q, SHI Y G, YANG J W, WANG S G, ZHANG W J, SUN D Z. Effects of low nitrogen stress on foxtail millet seedling characteristics and screening of low nitrogen tolerant varieties. Chinese Journal of Eco-Agriculture, 2020, 28(4): 523-534. (in Chinese)
[23]
LIAO M T, FILLERY I R P, PALTA J A. Early vigorous growth is a major factor influencing nitrogen uptake in wheat. Functional Plant Biology, 2004, 31(2): 121-129.

doi: 10.1071/FP03060 pmid: 32688884
[24]
郑玉冲, 张琳琦, 刘彬彬. 不同小麦品种根区微生物特征及对土壤氮素水平的响应. 中国生态农业学报(中英文), 2023, 31(11): 1708-1720.
ZHENG Y C, ZHANG L Q, LIU B B. Characteristics of root- associated microbiomes and their responses to soil nitrogen levels in different wheat cultivars. Chinese Journal of Eco-Agriculture, 2023, 31(11): 1708-1720. (in Chinese)
[25]
李淑文, 文宏达, 周彦珍, 李雁鸣, 肖凯. 不同氮效率小麦品种氮素吸收和物质生产特性. 中国农业科学, 2006, 39(10): 1992-2000.
LI S W, WEN H D, ZHOU Y Z, LI Y M, XIAO K. Characterization of nitrogen uptake and dry matter production in wheat varieties with different N efficiency. Scientia Agricultura Sinica, 2006, 39(10): 1992-2000. (in Chinese)

doi: 10.3864/j.issn.0578-1752.at-2005-6069
[26]
张恒, 陈艳琦, 任杰莹, 杨洪坤, 樊高琼. 西南麦区小麦苗期氮高效品种筛选及指标体系构建. 四川农业大学学报, 2022, 40(1): 10-18, 27.
ZHANG H, CHEN Y Q, REN J Y, YANG H K, FAN G Q. Screening of wheat cultivars with high nitrogen efficiency at seedling stage and construction of index system in southwest wheat region. Journal of Sichuan Agricultural University, 2022, 40(1): 10-18, 27. (in Chinese)
[27]
张士昌, 史占良, 李孟军, 李亚青, 底瑞耀, 李雁鸣. 长期定位氮胁迫对小麦碳氮代谢、氮素利用及产量的影响. 河南农业科学, 2016, 45(12): 13-19.
ZHANG S C, SHI Z L, LI M J, LI Y Q, DI R Y, LI Y M. Effect of long-term nitrogen stress on carbon and nitrogen metabolism, nitrogen use efficiency and yield of wheat. Journal of Henan Agricultural Sciences, 2016, 45(12): 13-19. (in Chinese)
[28]
张凡, 袁澍, 雷韬, 刘自礼, 杜俊波, 王建辉, 胡朝阳, 梁厚果, 林宏辉. 大量元素缺乏对小麦光合、呼吸作用和生理特性的影响. 四川大学学报(自然科学版), 2009, 46(2): 462-468.
ZHANG F, YUAN S, LEI T, LIU Z L, DU J B, WANG J H, HU Z Y, LIANG H G, LIN H H. The effects of macronutrient deficiency on photosynthetic activity, respiration and physiological parameters in wheat. Journal of Sichuan University (Natural Science Edition), 2009, 46(2): 462-468. (in Chinese)
[29]
王小纯, 程振云, 何建国, 熊淑萍, 马新明. 不同氮素形态对专用小麦苗期氨同化关键酶活性的影响. 麦类作物学报, 2008, 28(5): 836-840.
WANG X C, CHENG Z Y, HE J G, XIONG S P, MA X M. Effects of nitrogen forms on the activities of key enzymes for NH4+ assimilation at seedling stage of different wheat cultivars with special-end use. Journal of Triticeae Crops, 2008, 28(5): 836-840. (in Chinese)
[30]
李宁, 高丽锋, 黄鑫, 史华伟, 杨进文, 史雨刚, 陈明, 贾继增, 孙黛珍. 耐低氮小麦品种的筛选及耐低氮指数的全基因组关联分析. 中国农业科学, 2025, 58(13): 2487-2503. doi: 10.3864/j.issn.0578-1752.2025.13.001.
LI N, GAO L F, HUANG X, SHI H W, YANG J W, SHI Y G, CHEN M, JIA J Z, SUN D Z. Screening of wheat varieties with low nitrogen tolerance and genome-wide association studies of low nitrogen stress tolerance index. Scientia Agricultura Sinica, 2025, 58(13): 2487-2503. doi: 10.3864/j.issn.0578-1752.2025.13.001. (in Chinese)
[31]
向爱慧, 白荣基, 郝宇琼, 赵佳佳, 武棒棒, 李晓华, 郑兴卫, 关攀锋, 郑军. 山西小麦矮秆基因的鉴定及株高遗传位点挖掘. 中国农业科学, 2025, 58(17): 3372-3388. doi: 10.3864/j.issn.0578-1752.2025.17.002.
XIANG A H, BAI R J, HAO Y Q, ZHAO J J, WU B B, LI X H, ZHENG X W, GUAN P F, ZHENG J. Identification of dwarf genes and mining of plant height genetic loci in Shanxi wheat. Scientia Agricultura Sinica, 2025, 58(17): 3372-3388. doi: 10.3864/j.issn.0578-1752.2025.17.002. (in Chinese)
[32]
顾鹏程, 姚立蓉, 张宏, 汪军成, 王昊, 马艳, 李玉刚, 李葆春. 不同小麦品种苗期氮利用效率评价及TaSPX1基因表达分析. 麦类作物学报, 2025, 45(7): 902-912.
GU P C, YAO L R, ZHANG H, WANG J C, WANG H, MA Y, LI Y G, LI B C. Evaluation of nitrogen use efficiency and expression analysis of TaSPX1 gene in different wheat varieties at seedling stage. Journal of Triticeae Crops, 2025, 45(7): 902-912. (in Chinese)
[33]
LYNCH J M, PANTING L M. Effects of season, cultivation and nitrogen fertiliser on the size of the soil microbial biomass. Journal of the Science of Food and Agriculture, 1982, 33(3): 249-252.

doi: 10.1002/jsfa.v33:3
[34]
陈旭, 杨习文, 李文, 周苏玫, 徐凤丹, 贺德先. 不同氮素利用效率小麦苗期的根系形态数量性状分析. 麦类作物学报, 2021, 41(2): 174-182.
CHEN X, YANG X W, LI W, ZHOU S M, XU F D, HE D X. Analysis on morphological and quantitative seedling root traits of cultivars with different nitrogen use efficiency in wheat (Triticum aestivum L.). Journal of Triticeae Crops, 2021, 41(2): 174-182. (in Chinese)
[1] WANG XiaoWei, DU FoLi, YAN HongCai, LANG ZhengDong, DANG ZhiJuan, LI BaoChun, WANG JunCheng, MA XiaoLe, WANG HuaJun, ZHANG Hong, YAO LiRong. Evaluation of Drought Resistance of 396 Spring Wheat Varieties at Grain Filling Stage and Maturity Stage [J]. Scientia Agricultura Sinica, 2026, 59(8): 1608-1621.
[2] JIAO WenJuan, HE WanLong, GENG HongWei, BAI Bin, LI JianFeng, CHENG YuKun. Stripe Rust Resistance Evaluation and Molecular Characterization of Yr Genes for 155 Spring Wheat Varieties (Lines) [J]. Scientia Agricultura Sinica, 2026, 59(5): 937-950.
[3] WEI WenLong, WANG LinLin, CHEN XiaoLi, WU LingBin, LI LuFang, XIE YunTing, WANG JiangWen. Effects of Partial Organic Fertilizer Substitution and Plastic Film Mulching on Root Growth, Yield, and Water Use Efficiency of Spring Wheat in Dryland [J]. Scientia Agricultura Sinica, 2026, 59(11): 2340-2357.
[4] MU ShuJia, DONG LiXia, LI Guang, YAN ZhenGang, LU YuLan. Optimization of N2O Emission Parameters in Dryland Spring Wheat Farmland Soil Based on Whale Optimization Algorithm [J]. Scientia Agricultura Sinica, 2025, 58(3): 537-547.
[5] QIU HaiLong, LI Pan, ZHANG DianKai, FAN ZhiLong, HU FaLong, CHEN GuiPing, FAN Hong, HE Wei, YIN Wen, ZHAO LianHao. Compensatory Effects of Multiple Cropping Green Manure on Growth and Yield Loss of Nitrogen-Reduced Spring Wheat in Oasis Irrigation Areas of Northwest China [J]. Scientia Agricultura Sinica, 2025, 58(3): 443-459.
[6] QIAN ZhiJin, WANG XiNa, TIAN HaiMei, WANG YueMei, HAO WenYue, ZHOU Hui, TAN JunLi. Effects of Continuous Reduction Fertilization on the Stability of Spring Wheat Grain Quality in the Yellow River Irrigation Area of Ningxia [J]. Scientia Agricultura Sinica, 2025, 58(22): 4703-4717.
[7] ZHANG HuanHuan, ZHANG DiaoLiang, WANG XiaoLi, CHEN Han, SHAO Juan, YIN Wen, HU FaLong, CHAI Qiang, FAN ZhiLong. Effects of Green Manure and Wheat Straw Combined Returning Application on Photosynthetic Characteristics and Yield of Spring Wheat Under Reduced Nitrogen Levels [J]. Scientia Agricultura Sinica, 2025, 58(17): 3461-3472.
[8] LIU PengPeng, LI JiangBo, XU HongJun, NIE YingBin, HAN XinNian, KONG DeZhen, SANG Wei. Genetic Diversity Analysis of Protein Fractions and Quality in Xinjiang Winter Wheat Cultivar Resources [J]. Scientia Agricultura Sinica, 2025, 58(15): 2948-2959.
[9] CAO JingWen, NIE ZhiGang, LI Guang, YANG Jie. Multi-Objective Optimization of Stable Yield and Emission Reduction of Dryland Spring Wheat Based on DNDC and NSGA-Ⅲ. Coupling Model [J]. Scientia Agricultura Sinica, 2025, 58(13): 2538-2551.
[10] LI Rong, LI ZhengPeng, YAN QingBiao, GUO RanRan, HAN Mei, XU Ke. Effects of Multiple Cropping Green Manure Combined with Different Nitrogen Fertilizer Levels on Yield and Quality of Spring Wheat [J]. Scientia Agricultura Sinica, 2025, 58(12): 2333-2345.
[11] ZHOU Quan, LU QiuMei, ZHAO ZhangChen, WU ChenRan, FU XiaoGe, ZHAO YuJiao, HAN Yong, LIN HuaiLong, CHEN WeiLin, MOU LiMing, LI XingMao, WANG ChangHai, HU YinGang, CHEN Liang. Identification of Drought Resistance of 244 Spring Wheat Varieties at Seedling Stage [J]. Scientia Agricultura Sinica, 2024, 57(9): 1646-1657.
[12] LI YongFei, LI ZhanKui, ZHANG ZhanSheng, CHEN YongWei, KANG JianHong, WU HongLiang. Effects of Postponing Nitrogen Fertilizer Application on Flag Leaf Physiological Characteristics and Yield of Spring Wheat Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2024, 57(8): 1455-1468.
[13] WANG YueMei, TIAN HaiMei, WANG XiNa, HAO WenYue, LÜ ZheMing, YU JinMing, TAN JunLi, WANG ZhaoHui. Effect of Continuous Reduction of Fertilizer Application on Yield Stability of Spring Wheat in Yellow River Irrigation Area of Ningxia [J]. Scientia Agricultura Sinica, 2024, 57(3): 539-554.
[14] YANG Chun, YANG DaiXing, LI Yan, LIANG SiHui, DENG XiaoQiang, QIAO DaHe, CHEN Juan, GUO Yan, LIN KaiQin, CHEN ZhengWu. Comprehensive Analysis of Morphologic Characters and Biochemical Components of Guizhou Dashu Tea Germplasms [J]. Scientia Agricultura Sinica, 2024, 57(19): 3894-3916.
[15] ZHOU HanMi, MA LinShuang, SUN QiLi, CHEN JiaGeng, LI JiChen, SU YuMin, CHEN Cheng, WU Qi. Optimization of Integrated Water and Nitrogen Regulation System in Apple Based on Multi-Objective Comprehensive Evaluation [J]. Scientia Agricultura Sinica, 2024, 57(18): 3654-3670.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd